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Recruitment of SH-Containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a Maleimide Group

  • This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.

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Author:Ivan Haralampiev, Monique Mertens, Roland Schwarzer, Andreas Herrmann, Rudolf Volkmer, Pablo WessigORCiDGND, Peter Mueller
ISSN:1433-7851 (print)
ISSN:1521-3773 (online)
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=25417776
Parent Title (English):Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
Place of publication:Weinheim
Document Type:Article
Year of first Publication:2015
Year of Completion:2015
Release Date:2017/03/27
Tag:liposomes; maleimide; membranes; palmitic acid; palmitoylation; peptides
First Page:323
Last Page:326
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert