Molecularly imprinted polymer-based electrochemical sensors for biopolymers
- Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one ‘separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.
Author details: | Frieder W. SchellerORCiDGND, Xiaorong ZhangGND, Aysu YarmanORCiDGND, Ulla WollenbergerORCiDGND, Róbert E. GyurcsányiORCiD |
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DOI: | https://doi.org/10.1016/j.coelec.2018.12.005 |
ISSN: | 2451-9103 |
Title of parent work (English): | Current opinion in electrochemistry |
Publisher: | Elsevier |
Place of publishing: | Amsterdam |
Publication type: | Review |
Language: | English |
Date of first publication: | 2018/12/14 |
Publication year: | 2019 |
Release date: | 2021/03/05 |
Tag: | Biomarker; Direct electron transfer; Electropolymerization; Epitope imprinting; Redox marker |
Volume: | 14 |
Number of pages: | 7 |
First page: | 53 |
Last Page: | 59 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG, Germany)German Research Foundation (DFG) [EXC 314]; ERA-Chemistry 2014 [61133]; NKFIH OTKA (Hungary)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [NN117637]; BME-Nanotechnology (Hungary) FIKP grant of EMMI (BME FIKP-NAT) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Green Open-Access |