- The essential micronutrient selenium (Se) is required for various systemic functions, but its beneficial range is narrow and overexposure may result in adverse health effects. Additionally, the chemical form of the ingested selenium contributes crucially to its health effects. While small Se species play a major role in Se metabolism, their toxicological effects, bioavailability and metabolic transformations following elevated uptake are poorly understood. Utilizing the tractable invertebrate Caenorhabditis elegans allowed for an alternative approach to study species-specific characteristics of organic and inorganic Se forms in vivo, revealing remarkable species-dependent differences in the toxicity and bioavailability of selenite, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys). An inverse relationship was found between toxicity and bioavailability of the Se species, with the organic species displaying a higher bioavailability than the inorganic form, yet being less toxic. Quantitative Se speciation analysis withThe essential micronutrient selenium (Se) is required for various systemic functions, but its beneficial range is narrow and overexposure may result in adverse health effects. Additionally, the chemical form of the ingested selenium contributes crucially to its health effects. While small Se species play a major role in Se metabolism, their toxicological effects, bioavailability and metabolic transformations following elevated uptake are poorly understood. Utilizing the tractable invertebrate Caenorhabditis elegans allowed for an alternative approach to study species-specific characteristics of organic and inorganic Se forms in vivo, revealing remarkable species-dependent differences in the toxicity and bioavailability of selenite, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys). An inverse relationship was found between toxicity and bioavailability of the Se species, with the organic species displaying a higher bioavailability than the inorganic form, yet being less toxic. Quantitative Se speciation analysis with HPLC/mass spectrometry revealed a partial metabolism of SeMet and MeSeCys. In SeMet exposed worms, identified metabolites were Se-adenosylselenomethionine (AdoSeMet) and Se-adenosylselenohomocysteine (AdoSeHcy), while worms exposed to MeSeCys produced Se-methylselenoglutathione (MeSeGSH) and -glutamyl-MeSeCys (-Glu-MeSeCys). Moreover, the possible role of the sole selenoprotein in the nematode, thioredoxin reductase-1 (TrxR-1), was studied comparing wildtype and trxr-1 deletion mutants. Although a lower basal Se level was detected in trxr-1 mutants, Se toxicity and bioavailability following acute exposure was indistinguishable from wildtype worms. Altogether, the current study demonstrates the suitability of C. elegans as a model for Se species dependent toxicity and metabolism, while further research is needed to elucidate TrxR-1 function in the nematode.…
MetadatenVerfasserangaben: | Isabelle RohnGND, Talke Anu Marschall, Nina KröpflORCiD, Kenneth Bendix Jensen, Michael AschnerORCiDGND, Simon Tuck, Doris KuehneltORCiD, Tanja SchwerdtleORCiDGND, Julia BornhorstORCiDGND |
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DOI: | https://doi.org/10.1039/c8mt00066b |
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ISSN: | 1756-5901 |
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ISSN: | 1756-591X |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29770420 |
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Titel des übergeordneten Werks (Englisch): | Metallomics : integrated biometal science |
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Verlag: | Royal Society of Chemistry |
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Verlagsort: | Cambridge |
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Publikationstyp: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Erstveröffentlichung: | 14.05.2018 |
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Erscheinungsjahr: | 2018 |
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Datum der Freischaltung: | 24.11.2021 |
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Band: | 10 |
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Ausgabe: | 6 |
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Seitenanzahl: | 10 |
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Erste Seite: | 818 |
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Letzte Seite: | 827 |
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Fördernde Institution: | German Research Foundation (DFG)German Research Foundation (DFG) [SCHW 903/9-1, BO 4103/2-1]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [I 2262-N28]; DFG Research Unit TraceAge [FOR 2558]; NIH Office of Research Infrastructure Programs [P40 OD010440]; National Institute of Environmental Health Sciences - NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Environmental Health Sciences (NIEHS) [R01ES10563, R01ES07331] |
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Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
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DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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