Enzymatic Degradation of Oligo(epsilon-caprolactone)s End-Capped with Phenylboronic Acid Derivatives at the Air-Water Interface
- The influence of terminal functionalization of oligo(epsilon-caprolactone)s (OCL) with phenylboronic acid pinacol ester or phenylboronic acid on the enzymatic degradation behavior at the air-water interface is investigated by the Langmuir monolayer degradation technique. While the unsubstituted OCL immediately degrades after injection of the enzyme lipase from Pseudomonas cepacia, enzyme molecules are incorporated into the films based on end-capped OCL before degradation. This incorporation of enzymes does not inhibit or suppress the film degradation, but retards it significantly. A specific binding of lipase to the polymer monolayer allows studying the enzymatic activity of bound proteins and the influence on the degradation process. The functionalization of a macromolecule with phenyl boronic acid groups is an approach to investigate their interactions with diol-containing biomolecules like sugars and to monitor their specified impact on the enzymatic degradation behavior at the air-water interface.
Author details: | Joana Rossberg, Falko O. RottkeORCiD, Burkhard SchulzORCiDGND, Andreas LendleinORCiDGND |
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DOI: | https://doi.org/10.1002/marc.201600471 |
ISSN: | 1022-1336 |
ISSN: | 1521-3927 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27762464 |
Title of parent work (English): | Macromolecular rapid communications |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Year of first publication: | 2016 |
Publication year: | 2016 |
Release date: | 2020/03/22 |
Volume: | 37 |
Number of pages: | 6 |
First page: | 1966 |
Last Page: | 1971 |
Funding institution: | Helmholtz-Association |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |