Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite
- Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 mu M for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.
| Author details: | Sören MeyerORCiD, Aniceto Lopez-Serrano, Hanna Mitze, Norbert JakubowskiORCiD, Tanja SchwerdtleORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1039/c7mt00285h |
| ISSN: | 1756-5901 |
| ISSN: | 1756-591X |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29292446 |
| Title of parent work (English): | Metallomics : integrated biometal science |
| Publisher: | Royal Society of Chemistry |
| Place of publishing: | Cambridge |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2017/12/13 |
| Publication year: | 2017 |
| Release date: | 2022/04/04 |
| Volume: | 10 |
| Issue: | 1 |
| Number of pages: | 4 |
| First page: | 73 |
| Last Page: | 76 |
| Funding institution: | NutriAct - Competence Cluster Nutrition Research Berlin-Potsdam - Federal Ministry of Education and Research [FKZ: 01EA1408B]; TraceAge DFG Research Unit on Interactions of essential trace elements in healthy and diseased elderly, Potsdam-Berlin-Jena [FOR 2558/1]; Marie Curie ActionsEuropean Union (EU) |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
| DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
