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Toxin-antigen conjugates as selection tools for antibody producing cells

  • The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.

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Author details:Katrin MesserschmidtORCiDGND, Katja Heilmann
DOI:https://doi.org/10.1016/j.jim.2012.10.010
ISSN:0022-1759
Title of parent work (English):Journal of immunological methods
Publisher:Elsevier
Place of publishing:Amsterdam
Publication type:Article
Language:English
Year of first publication:2013
Publication year:2013
Release date:2017/03/26
Tag:Hybridoma technology; Monoclonal antibody; Selection of antibody producing cells
Volume:387
Issue:1-2
Number of pages:6
First page:167
Last Page:172
Funding institution:Federal Ministry of Education and Research [703]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer review:Referiert
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