Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance
- A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.
Author details: | Ruben R. RosencrantzORCiDGND, Vu Hoa Nguyen, Hyunji Park, Christine Schulte, Alexander BökerORCiDGND, Uwe Schnakenberg, Lothar Elling |
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DOI: | https://doi.org/10.1007/s00216-016-9667-9 |
ISSN: | 1618-2642 |
ISSN: | 1618-2650 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27277814 |
Title of parent work (English): | Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis |
Publisher: | Springer |
Place of publishing: | Heidelberg |
Publication type: | Article |
Language: | English |
Year of first publication: | 2016 |
Publication year: | 2016 |
Release date: | 2020/03/22 |
Tag: | Bacterial toxin; Biosensors; Glycopolymer brush; Glycosyltransferase; Localized surface plasmon resonance; Microfluidics |
Volume: | 408 |
Number of pages: | 8 |
First page: | 5633 |
Last Page: | 5640 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG) [SFB 985] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |