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MIPs and aptamers for recognition of proteins in biomimetic sensing

  • Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either "evolution in the test tube" of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified aptamers and ionic strength-dependentBiomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either "evolution in the test tube" of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.show moreshow less

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Metadaten
Author:Marcus Menger, Aysu Yarman, Júlia Erdőssy, Huseyin Bekir Yildiz, Róbert E. Gyurcsányi, Frieder W. SchellerORCiDGND
URN:urn:nbn:de:kobv:517-opus4-400496
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (357)
Document Type:Postprint
Language:English
Date of first Publication:2017/09/22
Year of Completion:2017
Publishing Institution:Universität Potsdam
Release Date:2017/09/22
Tag:SELEX; aptamers; aptasensors; biomimetic recognition elements; chemical sensors; in vitro selection; molecularly imprinted polymers
Pagenumber:19
Source:Biosensors 6 (2016) Nr. 3. - DOI: 10.3390/bios6030035
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Peer Review:Referiert
Publication Way:Open Access
Grantor:Multidisciplinary Digital Publishing Institute (MDPI)
Licence (German):License LogoCreative Commons - Namensnennung, 4.0 International