An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
- A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen- sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong auto fluorescence of the plant tissue. The method was established using photosynthesis- competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species
| Author details: | E. Schmalzlin, J. T. van Dongen, I. Klimant, Bettina Marmodee, Martin SteupORCiDGND, Joachim Fisahn, Peter Ludwig Geigenberger, Hans-Gerd LöhmannsröbenORCiDGND |
|---|---|
| ISSN: | 0006-3495 |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2005 |
| Publication year: | 2005 |
| Release date: | 2017/03/24 |
| Source: | Biophysical Journal. - ISSN 0006-3495. - 89 (2005), 2, S. 1339 - 1345 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
