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An ion-controlled four-color fluorescent telomeric switch on DNA origami structures

  • The folding of single-stranded telomeric DNA into guanine (G) quadruplexes is a conformational change that plays a major role in sensing and drug targeting. The telomeric DNA can be placed on DNA origami nanostructures to make the folding process extremely selective for K+ ions even in the presence of high Na+ concentrations. Here, we demonstrate that the K+-selective G-quadruplex formation is reversible when using a cryptand to remove K+ from the G-quadruplex. We present a full characterization of the reversible switching between single-stranded telomeric DNA and G-quadruplex structures using Förster resonance energy transfer (FRET) between the dyes fluorescein (FAM) and cyanine3 (Cy3). When attached to the DNA origami platform, the G-quadruplex switch can be incorporated into more complex photonic networks, which is demonstrated for a three-color and a four-color FRET cascade from FAM over Cy3 and Cy5 to IRDye700 with G-quadruplex-Cy3 acting as a switchable transmitter.

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Metadaten
Author details:Lydia OlejkoORCiDGND, P. J. Cywiński, Ilko BaldORCiDGND
URN:urn:nbn:de:kobv:517-opus4-95831
Publication series (Volume number):Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (254)
Publication type:Postprint
Language:English
Date of first publication:2016/04/15
Publication year:2016
Publishing institution:Universität Potsdam
Release date:2016/09/15
Tag:3-color fret; assemblies; g-quadruplex; nanostructures; photonic wires; quantum dots; recognition; resonance energy-transfer; sensitivity; strand breakage
First page:10339
Last Page:10347
Source:Nanoscale (2016), Nr. 8, S. 10339-10347. DOI: 10.1039/C6NR00119J
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
DDC classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Peer review:Referiert
Publishing method:Open Access
License (English):License LogoCreative Commons - Namensnennung 3.0 Unported
External remark:Bibliographieeintrag der Originalveröffentlichung/Quelle
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