Inhibition of farnesoic acid methyltransferase by sinefungin
- Sinefungin inhibited the S-adenosylmethionine-dependent farnesoic acid methyltransferase in a cell-free system containing a homogenate of corpora allata from female locusts, Locusta migratoria. The enzyme catalyzed the penultimate step of juvenile hormone biosynthesis in the insects. Culturing corpora allata in the presence of sinefungin greatly suppressed juvenile hormone production. The following in vivo effects were visible after injection of the inhibitor: increase in mortality and reduction of total haemolymph protein liter and ovary fresh weight, as well as length of terminal oocytes. Attempts to reverse these effects by topical application of the juvenile hormone analog ZR-515 (methoprene) were only partly successful. Therefore, the in vivo effects may be due to a general inhibition of methyltransferase enzymes in the insect. Sinefungin appeared to be of potential interest as the first representative of a new class of insect growth regulators.
Author details: | Hans-Jürgen Ferenz, Martin G. Peter, Dieter Berg |
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URN: | urn:nbn:de:kobv:517-opus-17016 |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 077) |
Publication type: | Postprint |
Language: | English |
Publication year: | 1983 |
Publishing institution: | Universität Potsdam |
Release date: | 2008/09/17 |
Tag: | Biological activity; Biosynthesis; Corpora allata; Enzyme; Enzyme inhibitor; In vitro; Juvenile hormone; Juvenile hormone analogue; Orthoptera |
Source: | Agricultural and Biological Chemistry. - 50 (1986), 4, pp.1003-1008. - ISSN (online) 1881-1280, ISSN (print) 0002-1369 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
License (German): | Keine öffentliche Lizenz: Unter Urheberrechtsschutz |
External remark: | first published in: Agricultural and Biological Chemistry. - 50 (1986), 4, pp. 1003-1008 ISSN (online) 1881-1280 ISSN (print) 0002-1369 Japan Society for Bioscience, Biotechnology and Agrochemistry |