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Synthesis of membrane proteins in eukaryotic cell-free systems

  • Cell-free protein synthesis (CFPS) is a valuable method for the fast expression of difficult-to-express proteins as well as posttranslationally modified proteins. Since cell-free systems circumvent possible cytotoxic effects caused by protein overexpression in living cells, they significantly enlarge the scale and variety of proteins that can be characterized. We demonstrate the high potential of eukaryotic CFPS to express various types of membrane proteins covering a broad range of structurally and functionally diverse proteins. Our eukaryotic cell-free translation systems are capable to provide high molecular weight membrane proteins, fluorescent-labeled membrane proteins, as well as posttranslationally modified proteins for further downstream analysis.

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Metadaten
Author:Rita Sachse, Doreen Wüstenhagen, Maria Samalikova, Michael Gerrits, Frank Fabian BierORCiDGND, Stefan Kubick
DOI:https://doi.org/10.1002/elsc.201100235
ISSN:1618-0240 (print)
Parent Title (English):Engineering in life sciences : Industry, Environment, Plant, Food
Publisher:Wiley-Blackwell
Place of publication:Hoboken
Document Type:Article
Language:English
Year of first Publication:2013
Year of Completion:2013
Release Date:2017/03/26
Tag:Cell-free protein expression; In vitro protein synthesis; Labeled membrane proteins; Synthetic glycoprotein
Volume:13
Issue:1
Pagenumber:10
First Page:39
Last Page:48
Funder:German Ministry of Education and Research (BMBF) [0313965A, 0312039]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert