• search hit 4 of 6
Back to Result List

A kinesin-mediated mechanism that couples centrosomes to nuclei

  • The M-type kinesin isoform, Kif9, has recently been implicated in maintaining a physical connection between the centrosome and nucleus in Dictyostelium discoideum. However, the mechanism by which Kif9 functions to link these two organelles remains obscure. Here we demonstrate that the Kif9 protein is localized to the nuclear envelope and is concentrated in the region underlying the centrosome point of attachment. Nuclear anchorage appears mediated through a specialized transmembrane domain located in the carboxyl terminus. Kif9 interacts with microtubules in in vitro binding assays and effects an endwise depolymerization of the polymer. These results suggest a model whereby Kif9 is anchored to the nucleus and generates a pulling force that reels the centrosome up against the nucleus. This is a novel activity for a kinesin motor, one important for progression of cells into mitosis and to ensure centrosome-nuclear parity in a multinuclear environment.

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author:Irina Tikhonenko, Valentin Magidson, Ralph GräfORCiDGND, Alexey Khodjakov, Michael P. Koonce
DOI:https://doi.org/10.1007/s00018-012-1205-0
ISSN:1420-682X (print)
Parent Title (English):Cellular and molecular life sciences
Publisher:Springer
Place of publication:Basel
Document Type:Article
Language:English
Year of first Publication:2013
Year of Completion:2013
Release Date:2017/03/26
Tag:Centrosome; Dictyostelium; Kinesin; Microtubule
Volume:70
Issue:7
Pagenumber:12
First Page:1285
Last Page:1296
Funder:NSF [MCB-1051612]; NIH [GM59363]; Deutsche Forschungsgemeinschaft [DFG GR1642/3-1]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert