• search hit 3 of 4
Back to Result List

Mechanisms of Hg species induced toxicity in cultured human astrocytes

  • The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co- genotoxic effect is likely due to a disturbance of the cellular DNA damage response.The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co- genotoxic effect is likely due to a disturbance of the cellular DNA damage response. Thus, at nanomolar, sub-cytotoxic concentrations, all three mercury species strongly disturbed poly(ADP-ribosyl)ation, a signalling reaction induced by DNA strand breaks. Interestingly, the molecular mechanism behind this inhibition seems to be different for the species. Since chronic PARP-1 inhibition is also discussed to sacrifice neurogenesis and learning abilities, further experiments on neurons and in vivo studies could be helpful to clarify whether the inhibition of poly(ADP-ribosyl) ation contributes to organic Hg induced neurotoxicity.show moreshow less

Download full text files

  • pmnr171.pdfeng
    (1764KB)

    SHA-1: a272521a8b78908138cf75db6ae96571341e200f

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author:Imke Pieper, Christoph A. Wehe, Julia Bornhorst, Franziska Ebert, Larissa Leffers, Michael Holtkamp, Pia Höseler, Till Weber, Aswin Mangerich, Alexander Bürkle, Uwe Karst, Tanja Schwerdtle
URN:urn:nbn:de:kobv:517-opus4-74379
Subtitle (English):genotoxicity and DNA-damage response
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 171)
Document Type:Postprint
Language:English
Date of first Publication:2014/02/03
Year of Completion:2014
Publishing Institution:Universität Potsdam
Release Date:2015/03/25
Tag:adduct formation; cell-death; exposure; manganese; methylmercury; neurodegenerative diseases; neurotoxicity; poly(ADP-ribose) polymerase-1; repair; thimerosal
Pagenumber:10
First Page:662
Last Page:671
Source:Metallomics (2014) 6, S. 662-671. - DOI: 10.1039/c3mt00337j
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Peer Review:Referiert
Publication Way:Open Access
Notes extern:Bibliographieeintrag der Originalveröffentlichung/Quelle