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Monopolar vs. bipolar subretinal stimulation-An in vitro study

  • This study uses an in vitro rd10 mouse model to quantify and compare the ability of the monopolar and the (concentric) bipolar electrode configurations for subretinal stimulation. To allow for results which can be directly compared an identical region of the retina was stimulated due to the circumstance that the bipolar electrode configuration allows also for monopolar stimulation, if the concentric counter-electrode is set potential-free (floating). A ganglion cell, located centrally over the bipolar electrode configuration was selected to extracellularly record action potentials during stimulation. To analyse the recorded action potentials, we introduce a new method which combines the advantages of (a) singular value decomposition (SVD) for weighting similar modulation patterns with which the recorded action potentials are characterized and (b) multi curve fitting to identify a common threshold level, required to finally assemble a strength-duration relationship (SDR). By directly comparing the obtained SDR curves, we found that theThis study uses an in vitro rd10 mouse model to quantify and compare the ability of the monopolar and the (concentric) bipolar electrode configurations for subretinal stimulation. To allow for results which can be directly compared an identical region of the retina was stimulated due to the circumstance that the bipolar electrode configuration allows also for monopolar stimulation, if the concentric counter-electrode is set potential-free (floating). A ganglion cell, located centrally over the bipolar electrode configuration was selected to extracellularly record action potentials during stimulation. To analyse the recorded action potentials, we introduce a new method which combines the advantages of (a) singular value decomposition (SVD) for weighting similar modulation patterns with which the recorded action potentials are characterized and (b) multi curve fitting to identify a common threshold level, required to finally assemble a strength-duration relationship (SDR). By directly comparing the obtained SDR curves, we found that the efficiency of stimulation with the monopolar electrode configuration is significantly higher than with the bipolar electrode configuration. All obtained SDR curves were fitted using the Lapicque model to estimate the chronaxie times and the rheobase currents. Liquid inclusions, eventually separating the retina from the electrodes are discussed to be a major cause for low ganglion cell responses during stimulation with the bipolar electrode configuration.show moreshow less

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Metadaten
Author:Matthias Gerhardt, Gillian Groeger, Niall MacCarthy
DOI:https://doi.org/10.1016/j.jneumeth.2011.04.017
ISSN:0165-0270 (print)
Parent Title (English):Journal of neuroscience methods
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of first Publication:2011
Year of Completion:2011
Release Date:2017/03/26
Tag:Bipolar; Cells; Electrodes; Ganglion; Monopolar; Retina; SVD; Subretinal
Volume:199
Issue:1
Pagenumber:9
First Page:26
Last Page:34
Funder:European Commission [MTKD-CT-2005-029568]; Fighting Blindness Ireland
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie
Peer Review:Referiert