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Microfluidic tools for quantitative studies of eukaryotic chemotaxis

  • Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.

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Metadaten
Author:Carsten BetaORCiDGND, Eberhard Bodenschatz
DOI:https://doi.org/10.1016/j.ejcb.2011.05.006
ISSN:0171-9335 (print)
Parent Title (English):European journal of cell biology
Publisher:Elsevier
Place of publication:Jena
Document Type:Review
Language:English
Year of first Publication:2011
Year of Completion:2011
Release Date:2017/03/26
Tag:Caged compounds; Dictyostelium discoideum; Eukaryotic chemotaxis; Microfluidics; Numerical simulations
Volume:90
Issue:10
Pagenumber:6
First Page:811
Last Page:816
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie
Peer Review:Referiert