Kolja V. Szymanski, Mario Tönnies, Anne Becher, Diana Fatykhova, Philippe D. N'Guessan, Birgitt Gutbier, Frederick Klauschen, Frank Neuschäfer-Rube, Paul Schneider, Jens Rückert, Jens Neudecker, Torsten T. Bauer, Klaus Dalhoff, Daniel Droemann, Achim D. Gruber, Olivia Kershaw, Bettina Temmesfeld-Wollbrueck, Norbert Suttorp, Stefan Hippenstiel, Andreas C. Hocke
- The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue.
Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites.
In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies,The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue.
Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites.
In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E-2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection.
Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E-2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections.…
MetadatenAuthor details: | Kolja V. Szymanski, Mario Tönnies, Anne Becher, Diana Fatykhova, Philippe D. N'Guessan, Birgitt Gutbier, Frederick Klauschen, Frank Neuschäfer-Rube, Paul Schneider, Jens Rückert, Jens Neudecker, Torsten T. Bauer, Klaus Dalhoff, Daniel Droemann, Achim D. Gruber, Olivia Kershaw, Bettina Temmesfeld-Wollbrueck, Norbert Suttorp, Stefan HippenstielGND, Andreas C. Hocke |
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DOI: | https://doi.org/10.1183/09031936.00186911 |
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ISSN: | 0903-1936 |
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Title of parent work (English): | The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology |
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Publisher: | European Respiratory Society |
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Place of publishing: | Sheffield |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2012 |
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Publication year: | 2012 |
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Release date: | 2017/03/26 |
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Tag: | Alveolar epithelial cells; cytokines; inflammation; lung infection; pneumonia; prostaglandins |
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Volume: | 40 |
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Issue: | 6 |
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Number of pages: | 10 |
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First page: | 1458 |
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Last Page: | 1467 |
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Funding institution: | Transregional Collaborative Research Center of the Deutsche
Forschungsgemeinschaft [SFB-TR84]; German Federal Ministry of Education
and Research (PROGRESS - Pneumonia Research Network on Genetic
Resistance and Susceptibility for the Evolution of Severe Sepsis) |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
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Peer review: | Referiert |
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