The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 2 of 15
Back to Result List

Determination of glucan phosphorylation using heteronuclear H-1,C-13 double and H-1,C-13,P-31 triple-resonance NMR spectra

  • Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear H-1,C-13 and H-1,C-13,P-31 techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.

Export metadata

Additional Services

Search Google Scholar Statistics
Metadaten
Author details:Peter Schmieder, Felix Nitschke, Martin SteupORCiDGND, Keven Mallow, Edgar Specker
DOI:https://doi.org/10.1002/mrc.3996
ISSN:0749-1581
Title of parent work (English):Magnetic resonance in chemistry
Publisher:Wiley-Blackwell
Place of publishing:Hoboken
Publication type:Article
Language:English
Year of first publication:2013
Publication year:2013
Release date:2017/03/26
Tag:heteronuclear NMR; phosphorylation; starch; triple resonance
Volume:51
Issue:10
Number of pages:7
First page:655
Last Page:661
Funding institution:Leibniz-Institut fur Molekulare Pharmakologie (FMP)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer review:Referiert
Accept ✔
This website uses technically necessary session cookies. By continuing to use the website, you agree to this. You can find our privacy policy here.