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Determination of glucan phosphorylation using heteronuclear H-1,C-13 double and H-1,C-13,P-31 triple-resonance NMR spectra

  • Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear H-1,C-13 and H-1,C-13,P-31 techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.

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Metadaten
Author:Peter Schmieder, Felix Nitschke, Martin Steup, Keven Mallow, Edgar Specker
DOI:https://doi.org/10.1002/mrc.3996
ISSN:0749-1581 (print)
Parent Title (English):Magnetic resonance in chemistry
Publisher:Wiley-Blackwell
Place of publication:Hoboken
Document Type:Article
Language:English
Year of first Publication:2013
Year of Completion:2013
Release Date:2017/03/26
Tag:heteronuclear NMR; phosphorylation; starch; triple resonance
Volume:51
Issue:10
Pagenumber:7
First Page:655
Last Page:661
Funder:Leibniz-Institut fur Molekulare Pharmakologie (FMP)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert