- New mesoporous silk fibroin (SF)/silica hybrids were processed via a one-pot soft and energy-efficient sol-gel chemistry and self-assembly from a silica precursor, an acidic or basic catalyst, and the ionic liquid 1-butyl-3-methylimidazolium chloride, acting as both solvent and mesoporosity-inducer. The as-prepared materials were obtained as slightly transparent-opaque, amorphous monoliths, easily transformed into powders, and stable up to ca. 300 degrees C. Structural data suggest the formation of a hexagonal mesostructure with low range order and apparent surface areas, pore volumes, and pore radii of 205-263 m(2) g(-1), 0.16-0.19 cm(3) g(-1), and 1.2-1.6 nm, respectively. In all samples, the dominating conformation of the SF chains is the beta-sheet. Cytotoxicity/bioactivity resazurin assays and fluorescence microscopy demonstrate the high viability of MC3T3 pre-osteoblasts to indirect (>= 99 +/- 9%) and direct (78 +/- 2 to 99 +/- 13%) contact with the SF/silica materials. Considering their properties and further improvements,New mesoporous silk fibroin (SF)/silica hybrids were processed via a one-pot soft and energy-efficient sol-gel chemistry and self-assembly from a silica precursor, an acidic or basic catalyst, and the ionic liquid 1-butyl-3-methylimidazolium chloride, acting as both solvent and mesoporosity-inducer. The as-prepared materials were obtained as slightly transparent-opaque, amorphous monoliths, easily transformed into powders, and stable up to ca. 300 degrees C. Structural data suggest the formation of a hexagonal mesostructure with low range order and apparent surface areas, pore volumes, and pore radii of 205-263 m(2) g(-1), 0.16-0.19 cm(3) g(-1), and 1.2-1.6 nm, respectively. In all samples, the dominating conformation of the SF chains is the beta-sheet. Cytotoxicity/bioactivity resazurin assays and fluorescence microscopy demonstrate the high viability of MC3T3 pre-osteoblasts to indirect (>= 99 +/- 9%) and direct (78 +/- 2 to 99 +/- 13%) contact with the SF/silica materials. Considering their properties and further improvements, these systems are promising candidates to be explored in bone tissue engineering. They also offer excellent prospects as electrolytes for solid-state electrochemical devices, in particular for fuel cells.…
MetadatenAuthor details: | Rui F. P. PereiraORCiD, Kerstin ZehbeORCiD, Christina GünterGND, Tiago dos Santos, Silvia C. Nunes, Filipe A. Almeida PazORCiD, Maria M. SilvaORCiD, Pedro L. Granja, Andreas TaubertORCiDGND, Verónica de Zea BermudezORCiD |
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DOI: | https://doi.org/10.1021/acsomega.8b02051 |
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ISSN: | 2470-1343 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30320252 |
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Title of parent work (English): | ACS Omega |
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Publisher: | American Chemical Society |
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Place of publishing: | Washington |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2018/09/07 |
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Publication year: | 2018 |
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Release date: | 2021/10/04 |
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Volume: | 3 |
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Issue: | 9 |
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Number of pages: | 12 |
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First page: | 10811 |
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Last Page: | 10822 |
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Funding institution: | Fundacao para a Ciencia e a Tecnologia (FCT)/Deutscher Akademischer Austauschdienst (DAAD) [6818]; national funds through the FCT/MEC [UID/CTM/50011/2013, POCI-01-0145-FEDER-007679]; FEDER under the PT2020 Partnership Agreement; project UniRCell - European Regional Development Fund (ERDF)European Union (EU) [POCI-01-0145-FEDER-016422, SAICTPAC/0032/2015]; FCTPortuguese Foundation for Science and Technology [UID/QUI/00686/2013, UID/QUI/00686/2016, PEst-OE/QUI/UI0616/2014, SFRH/BPD/87759/2012] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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