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Eight new microsatellite loci for the critically endangered fire-bellied toad Bombina bombina and their cross- species applicability among anurans

  • We describe eight new microsatellite loci for the critically endangered fire-bellied toad, Bombina bombina. Seven of them are polymorphic with two to seven alleles per locus, an expected heterozygosity between 0.41 and 0.8, and an observed heterozygosity between 0.27 and 0.7. The yield of new loci was relatively low, presumably due to mildly repetitive sequence motifs in microsatellite flanking regions. As typical for anurans, cross-species amplification was limited (here, to congeners Bombina orientalis and Bombina variegata). Combining these new loci with those already available provides a reasonable number of loci for population studies and pedigree analysis in Bombina

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Author:H Stuckas, Ralph TiedemannORCiDGND
Document Type:Article
Year of first Publication:2006
Year of Completion:2006
Release Date:2017/03/25
Source:Molecular ecology notes. - 6 (2006), 1, S. 150 - 152
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:Referiert