Generation of murine monoclonal antibodies with specificity against conventional camelid IgG1 and heavy-chain only IgG2/3

  • Camelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in antigen binding. In this study subclass-specific murine monoclonal antibodies specific to conventional camelid IgG1 and heavy-chain only IgG2/3 were generated and validated for the use as potent secondary detection reagents. The monoclonal antibodies are able to differentiate between all camelid IgGs, conventional four-chain camelid antibodies (of subclass IgG1) and exclusively heavy chain-only antibodies (of subclasses IgG2 and IgG3). Further these antibodies were used to detect specific immune responses after vaccination of Camelids against bovine corona- and rotavirus strains and different E.coli. and Clostridia - antigens and to identify Erysipelothrix rhusiopathiae infected animals within a herd. The described antibodies are suitable as new secondary agents for the detection of differentCamelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in antigen binding. In this study subclass-specific murine monoclonal antibodies specific to conventional camelid IgG1 and heavy-chain only IgG2/3 were generated and validated for the use as potent secondary detection reagents. The monoclonal antibodies are able to differentiate between all camelid IgGs, conventional four-chain camelid antibodies (of subclass IgG1) and exclusively heavy chain-only antibodies (of subclasses IgG2 and IgG3). Further these antibodies were used to detect specific immune responses after vaccination of Camelids against bovine corona- and rotavirus strains and different E.coli. and Clostridia - antigens and to identify Erysipelothrix rhusiopathiae infected animals within a herd. The described antibodies are suitable as new secondary agents for the detection of different camelid subclasses and the validation of camelid immune reactions.show moreshow less

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Metadaten
Author details:Pamela Holzlöhner, Monique Butze, Natalia MaierGND, Nicole Hebel, Erik Schliebs, Burkhard Micheel, Jonas Fuener, Gabriele Heidicke, Katja HanackORCiDGND
DOI:https://doi.org/10.1016/j.vetimm.2018.01.006
ISSN:0165-2427
ISSN:1873-2534
Pubmed ID:http://www.ncbi.nlm.nih.gov/pubmed?term=29475500
Title of parent work (English):Veterinary Immunology and Immunopathology
Publisher:Elsevier
Place of publishing:Amsterdam
Publication type:Article
Language:English
Date of first publication:2018/01/30
Completion year:2018
Release date:2022/01/14
Tag:Camelid antibodies; Disease monitoring; Heavy-chain only antibodies; Monoclonal antibodies; Secondary antibodies; Vaccination
Volume:197
Number of pages:6
First page:1
Last Page:6
Funding institution:Federal Ministry of Economics and Technology (ZIM-Koop Program) [KF2144001SB8]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
DDC classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Peer review:Referiert
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