Ion-Selective formation of a guanine quadruplex on DNA origami structures

  • DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na+ and K+) is currently used for the detection of K+ ions, however, with insufficient selectivity towards Na+. By means of FRET between two suitable dyes attached to the 3- and 5-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.

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Author:Lydia OlejkoORCiDGND, Piotr J. Cywinski, Ilko BaldORCiDGND
ISSN:1433-7851 (print)
ISSN:1521-3773 (online)
Pubmed Id:
Parent Title (English):Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
Place of publication:Weinheim
Document Type:Article
Year of first Publication:2015
Year of Completion:2015
Release Date:2017/03/27
Tag:DNA nanotechnology; FRET; G-quadruplexes; nanostructures; self-assembly
First Page:673
Last Page:677
Funder:Marie Curie FP7 Integration Grant within the 7th European Union Framework Programme; Deutsche Forschungsgemeinschaft (DFG); University of Potsdam; DFG [GSC 1013]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert