TY - JOUR A1 - Haase, Tobias A1 - Krost, Annalena A1 - Sauter, Tilman A1 - Kratz, Karl A1 - Peter, Jan A1 - Kamann, Stefanie A1 - Jung, Friedrich A1 - Lendlein, Andreas A1 - Zohlnhöfer, Dietlind A1 - Rüder, Constantin T1 - In vivo biocompatibility assessment of poly (ether imide) electrospun scaffolds JF - Journal of Tissue Engineering and Regenerative Medicine N2 - Poly(ether imide) (PEI), which can be chemically functionalized with biologically active ligands, has emerged as a potential biomaterial for medical implants. Electrospun PEI scaffolds have shown advantageous properties, such as enhanced endothelial cell adherence, proliferation and low platelet adhesion in in vitro experiments. In this study, the in vivo behaviour of electrospun PEI scaffolds and PEI films was examined in a murine subcutaneous implantation model. Electrospun PEI scaffolds and films were surgically implanted subcutaneously in the dorsae of mice. The surrounding subcutaneous tissue response was examined via histopathological examination at 7 and 28days after implantation. No serious adverse events were observed for both types of PEI implants. The presence of macrophages or foreign body giant cells in the vicinity of the implants and the formation of a fibrous capsule indicated a normal foreign body reaction towards PEI films and scaffolds. Capsule thickness and inflammatory infiltration cells significantly decreased for PEI scaffolds during days 7-28 while remaining unchanged for PEI films. The infiltration of cells into the implant was observed for PEI scaffolds 7days after implantation and remained stable until 28days of implantation. Additionally some, but not all, PEI scaffold implants induced the formation of functional blood vessels in the vicinity of the implants. Conclusively, this study demonstrates the in vivo biocompatibility of PEI implants, with favourable properties of electrospun PEI scaffolds regarding tissue integration and wound healing. KW - poly(ether imide) KW - in vivo study KW - electrospun scaffold KW - capsule formation KW - foreign body giant cells KW - vascularization Y1 - 2017 U6 - https://doi.org/10.1002/term.2002 SN - 1932-6254 SN - 1932-7005 VL - 11 IS - 4 SP - 1034 EP - 1044 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Koc-Januchta, Marta A1 - Höffler, Tim A1 - Thoma, Gun-Brit A1 - Prechtl, Helmut A1 - Leutner, Detlev T1 - Visualizers versus verbalizers BT - Effects of cognitive style on learning with texts and pictures - An eye-tracking study JF - Computers in human behavior N2 - This study was conducted in order to examine the differences between visualizers and verbalizers in the way they gaze at pictures and texts while learning. Using a collection of questionnaires, college students were classified according to their visual or verbal cognitive style and were asked to learn about two different, in terms of subject and type of knowledge, topics by means of text-picture combinations. Eye-tracking was used to investigate their gaze behavior. The results show that visualizers spent significantly more time inspecting pictures than verbalizers, while verbalizers spent more time inspecting texts. Results also suggest that both visualizers' and verbalizers' way of learning is active but mostly within areas providing the source of information in line with their cognitive style (pictures or text). Verbalizers tended to enter non-informative, irrelevant areas of pictures sooner than visualizers. The comparison of learning outcomes showed that the group of visualizers achieved better results than the group of verbalizers on a comprehension test. KW - Cognitive style KW - Verbalizer KW - Visualizer KW - Eye-tracking KW - Multimedia learning Y1 - 2016 U6 - https://doi.org/10.1016/j.chb.2016.11.028 SN - 0747-5632 SN - 1873-7692 VL - 68 SP - 170 EP - 179 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Musalek, Martin A1 - Kokstejn, Jakub A1 - Papez, Pavel A1 - Scheffler, Christiane A1 - Mumm, Rebekka A1 - Czernitzki, Anna-Franziska A1 - Koziel, Slawomir T1 - Impact of normal weight obesity on fundamental motor skills in pre-school children aged 3 to 6 years JF - Journal of biological and clinical anthropology : Anthropologischer Anzeiger ; Mitteilungsorgan der Gesellschaft für Anthropologie N2 - Normal weight obesity is defined as having excessive body fat, but normal BMI. Even though previous research revealed that excessive body fat in children inhibited their physical activity and decreased motor performance, there has been only little evidence about motor performance of normal weight obese children. This study aims to establish whether normal weight obese pre-school children aged 3-6 years will have a significantly worse level of fundamental motor skills compared to normal weight non-obese counterparts. The research sample consisted of 152 pre-schoolers selected from a specific district of Prague, the Czech Republic. According to values from four skinfolds: triceps, subscapula, suprailiaca, calf, and BMI three categories of children aged 3-6 years were determined: A) normal weight obese n = 51; B) normal weight non-obese n = 52; C) overweight and obese n = 49. The Movement Assessment Battery for Children (MABC-2) was used for the assessment of fundamental motor skills. Normal weight obese children had significantly higher amount of adipose tissue p < 0.001 than normal weight non-obese children but the same average BMI. Moreover, normal weight obese children did not have significantly less amount of subcutaneous fat on triceps and calf compared to their overweight and obese peers. In majority of MABC-2 tests, normal weight obese pre-schoolers showed the poorest performance. Moreover, normal weight obese children had significantly worse total standard score = 38.82 compared to normal weight non-obese peers = 52.27; p < 0.05. In addition, normal weight obese children had a more than three times higher frequency OR = 3.69 CI95% (1.10; 12.35) of severe motor deficit performance <= 5th centile of the MABC-2 norm. These findings are strongly alarming since indices like BMI are not able to identify normal weight obese individual. We recommend verifying real portion of normal weight obese children as they are probably in higher risk of health and motor problems than overweight and obese population due to their low lean mass. KW - normal weight obesity KW - fundamental motor skills KW - MABC-2 KW - performance KW - pre-school children KW - skinfolds KW - adipose tissue KW - lean mass Y1 - 2017 U6 - https://doi.org/10.1127/anthranz/2017/0752 SN - 0003-5548 VL - 74 SP - 203 EP - 212 PB - Schweizerbart CY - Stuttgart ER - TY - JOUR A1 - Gomula, Aleksandra A1 - Koziel, Slawomir A1 - Groth, Detlef A1 - Bielicki, Tadeusz T1 - The effect of neighboring districts on body height of Polish conscripts JF - Anthropologischer Anzeiger : journal of biological and clinical anthropology ; Mitteilungsorgan der Gesellschaft für Anthropologie N2 - The aim of the study was to investigate the correlation of heights of conscripts living in neighboring districts in Poland. The study used 10% of a nationally representative sample of 26,178 males 18.5-19.5 years old examined during the National survey of Polish conscripts conducted in 2001. The sample represented all regions and social strata of the country and included 354 different districts within 16 voivodships (provinces). Analyses were performed with the R statistical software. A small but significant correlation (0.24, p < 0.0001) was observed for height between 1st order neighboring districts. Correlations decreased with increased distances between neighboring districts, but remained significant for 7th node neighbors (0.18, p < 0.0001). Regarding voivodships (provinces), average height showed a geographical trend from the northwest (relatively tall) to the southeast (relatively short), and the correlation was stronger for first order neighboring provinces (0.796, p < 0.001). This study revealed clusters of tall people and short people, providing a support for hypothesis of the community effect in height. Small correlations between 1st order neighbors than in another country (Switzerland) may be associated with differences in geography, since in Poland there are no natural barriers (e.g., mountains) and road infrastructure is well-developed. KW - body height KW - community effect KW - Polish conscripts Y1 - 2017 U6 - https://doi.org/10.1127/anthranz/2017/0701 SN - 0003-5548 SN - 2363-7099 VL - 74 IS - 1 SP - 71 EP - 76 PB - Schweizerbart CY - Stuttgart ER - TY - JOUR A1 - Holzlöhner, Pamela A1 - Hanack, Katja T1 - Generation of murine monoclonal antibodies by hybridoma technology JF - JoVE : Video journal N2 - Monoclonal antibodies are universal binding molecules and are widely used in biomedicine and research. Nevertheless, the generation of these binding molecules is time-consuming and laborious due to the complicated handling and lack of alternatives. The aim of this protocol is to provide one standard method for the generation of monoclonal antibodies using hybridoma technology. This technology combines two steps. Step 1 is an appropriate immunization of the animal and step 2 is the fusion of B lymphocytes with immortal myeloma cells in order to generate hybrids possessing both parental functions, such as the production of antibody molecules and immortality. The generated hybridoma cells were then recloned and diluted to obtain stable monoclonal cell cultures secreting the desired monoclonal antibody in the culture supernatant. The supernatants were tested in enzyme-linked immunosorbent assays (ELISA) for antigen specificity. After the selection of appropriate cell clones, the cells were transferred to mass cultivation in order to produce the desired antibody molecule in large amounts. The purification of the antibodies is routinely performed by affinity chromatography. After purification, the antibody molecule can be characterized and validated for the final test application. The whole process takes 8 to 12 months of development, and there is a high risk that the antibody will not work in the desired test system. KW - Immunology KW - Issue 119 KW - monoclonal antibodies KW - hybridoma technology KW - myeloma cells KW - B lymphocytes KW - antigen KW - immunconjugate Y1 - 2017 U6 - https://doi.org/10.3791/54832 SN - 1940-087X IS - 119 PB - JoVE CY - Cambridge ER - TY - JOUR A1 - Lütkecosmann, Steffi A1 - Warsinke, Axel A1 - Tschöpe, Winfried A1 - Eichler, Rüdiger A1 - Hanack, Katja T1 - A novel monoclonal antibody suitable for the detection of leukotriene B4 JF - Biochemical and biophysical research communications N2 - Leukotriene B4 as an inflammatory mediator is an important biomarker for different respiratory diseases like asthma, chronic obstructive pulmonary disease or cystic lung fibrosis. Therefore the detection of LTB4 is helpful in the diagnosis of these pulmonary diseases. However, until now its determination in exhaled breath condensates suffers from problems of accuracy. Reasons for that could be improper sample collection and preparation methods of condensates and the lack of consistently assay specificity and reproducibility of the used immunoassay detection system. In this study we describe the development and the characterization of a specific monoclonal antibody (S27BC6) against LTB4, its use as molecular recognition element for the development of an enzyme-linked immunoassay to detect LTB4 and discuss possible future diagnostic applications. KW - Leukotriene B4 KW - Monoclonal antibody KW - Immunosensor KW - Chronic obstructive pulmonary disease (COPD) KW - Hapten Y1 - 2017 U6 - https://doi.org/10.1016/j.bbrc.2016.11.157 SN - 0006-291X SN - 1090-2104 VL - 482 IS - 4 SP - 1054 EP - 1059 PB - Elsevier CY - San Diego ER - TY - THES A1 - de Abreu e Lima, Francisco Anastacio T1 - Experimental validation of hybrid performance predictive models in Zea mays L. Y1 - 2017 ER - TY - THES A1 - Bajdzienko, Krzysztof T1 - Analysis of Target of Rapamycin (Tor) induced changes of the Arabidopsis thaliana proteome using sub-cellular resolution Y1 - 2017 ER - TY - THES A1 - Kubsch, Bastian T1 - Phase-specific fusion between biomembranes using SNARE mimetics Y1 - 2017 ER - TY - THES A1 - Górka, Michal Jakub T1 - Establishing a pipeline for identification of protein- protein interactions using different native fractionation methods Y1 - 2017 ER - TY - THES A1 - Martínez Jaime, Silvia T1 - Towards the understanding of protein function and regulation BT - organization of the mitochondrial protein complexome under different conditions and the role of SUM03 in Arabidopsis thaliana Y1 - 2017 ER - TY - THES A1 - Yang, Lei T1 - Verification of systemic mRNAs mobility and mobile functions Y1 - 2017 ER - TY - THES A1 - Barbirz, Stefanie T1 - Highly specific binders for bacterial polysaccharides BT - bacteriophage tailspike proteins as research models in structural thermodynamics and bacteriophage infection Y1 - 2017 ER - TY - THES A1 - Diez Cocero, Mercedes T1 - Analysis of Rubisco - carbonic anhydrase fusions in tobacco as an approach to reduce photorespiration Y1 - 2017 ER - TY - THES A1 - Belkius, Karolina Dorota T1 - Systems biology approach to investigate the development and degradation of the photosynthetic apparatus during leaf ontogenesis in higher plants Y1 - 2017 ER - TY - THES A1 - Zeng, Ting T1 - Nanoparticles promoted biocatalysis BT - Electrochemical investigation of human sulfite oxidase on nanoparticles modified electrodes Y1 - 2017 ER - TY - THES A1 - Pandey-Pant, Pooja T1 - Comparative transcriptomics and functional genomics during phosphorus limitation in plants Y1 - 2017 ER - TY - JOUR A1 - Fer, Istem A1 - Tietjen, Britta A1 - Jeltsch, Florian A1 - Wolff, Christian Michael T1 - The influence of El Nino-Southern Oscillation regimes on eastern African vegetation and its future implications under the RCP8.5 warming scenario JF - Biogeosciences N2 - The El Nino-Southern Oscillation (ENSO) is the main driver of the interannual variability in eastern African rainfall, with a significant impact on vegetation and agriculture and dire consequences for food and social security. In this study, we identify and quantify the ENSO contribution to the eastern African rainfall variability to forecast future eastern African vegetation response to rainfall variability related to a predicted intensified ENSO. To differentiate the vegetation variability due to ENSO, we removed the ENSO signal from the climate data using empirical orthogonal teleconnection (EOT) analysis. Then, we simulated the ecosystem carbon and water fluxes under the historical climate without components related to ENSO teleconnections. We found ENSO-driven patterns in vegetation response and confirmed that EOT analysis can successfully produce coupled tropical Pacific sea surface temperature-eastern African rainfall teleconnection from observed datasets. We further simulated eastern African vegetation response under future climate change as it is projected by climate models and under future climate change combined with a predicted increased ENSO intensity. Our EOT analysis highlights that climate simulations are still not good at capturing rainfall variability due to ENSO, and as we show here the future vegetation would be different from what is simulated under these climate model outputs lacking accurate ENSO contribution. We simulated considerable differences in eastern African vegetation growth under the influence of an intensified ENSO regime which will bring further environmental stress to a region with a reduced capacity to adapt effects of global climate change and food security. Y1 - 2017 U6 - https://doi.org/10.5194/bg-14-4355-2017 SN - 1726-4170 SN - 1726-4189 VL - 14 IS - 18 SP - 4355 EP - 4374 PB - Copernicus CY - Katlenburg-Lindau ER - TY - THES A1 - Wu, Si T1 - Exploring the Arabidopsis metabolic landscape by genetic mapping integrated with network analysis Y1 - 2017 ER - TY - THES A1 - Loiacono, Filomena Vanessa T1 - Transfer of chloroplast RNA editing events between species BT - faithful reconstitution and fateful effects Y1 - 2017 ER -