TY  - JOUR
A1  - Liu, Hsiang-chin
A1  - Lämke, Jörn
A1  - Lin, Siou-ying
A1  - Hung, Meng-Ju
A1  - Liu, Kuan-Ming
A1  - Charng, Yee-yung
A1  - Bäurle, Isabel
T1  - Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress
JF  - The plant journal
N2  - Plants can be primed by a stress cue to mount a faster or stronger activation of defense mechanisms upon subsequent stress. A crucial component of such stress priming is the modified reactivation of genes upon recurring stress; however, the underlying mechanisms of this are poorly understood. Here, we report that dozens of Arabidopsis thaliana genes display transcriptional memory, i.e. stronger upregulation after a recurring heat stress, that lasts for at least 3 days. We define a set of transcription factors involved in this memory response and show that the transcriptional memory results in enhanced transcriptional activation within minutes of the onset of a heat stress cue. Further, we show that the transcriptional memory is active in all tissues. It may last for up to a week, and is associated during this time with histone H3 lysine 4 hypermethylation. This transcriptional memory is cis-encoded, as we identify a promoter fragment that confers memory onto a heterologous gene. In summary, heat-induced transcriptional memory is a widespread and sustained response, and our study provides a framework for future mechanistic studies of somatic stress memory in higher plants.
KW  - epigenetics
KW  - priming
KW  - heat stress
KW  - H3K4 methylation
KW  - transcriptional memory
KW  - Arabidopsis thaliana
KW  - HSF
Y1  - 2018
U6  - https://doi.org/10.1111/tpj.13958
SN  - 0960-7412
SN  - 1365-313X
VL  - 95
IS  - 3
SP  - 401
EP  - 413
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Bäurle, Isabel
A1  - Brzezinka, Krzysztof
A1  - Altmann, Simone
T1  - BRUSHY1/TONSOKU/MGOUN3 is required for heat stress memory
JF  - Plant Cell & Environment
N2  - Plants encounter biotic and abiotic stresses many times during their life cycle and this limits their productivity. Moderate heat stress (HS) primes a plant to survive higher temperatures that are lethal in the naïve state. Once temperature stress subsides, the memory of the priming event is actively retained for several days preparing the plant to better cope with recurring HS. Recently, chromatin regulation at different levels has been implicated in HS memory. Here, we report that the chromatin protein BRUSHY1 (BRU1)/TONSOKU/MGOUN3 plays a role in the HS memory in Arabidopsis thaliana. BRU1 is also involved in transcriptional gene silencing and DNA damage repair. This corresponds with the functions of its mammalian orthologue TONSOKU‐LIKE/NFΚBIL2. During HS memory, BRU1 is required to maintain sustained induction of HS memory‐associated genes, whereas it is dispensable for the acquisition of thermotolerance. In summary, we report that BRU1 is required for HS memory in A. thaliana, and propose a model where BRU1 mediates the epigenetic inheritance of chromatin states across DNA replication and cell division.
KW  - Arabidopsis thaliana
KW  - BRUSHY1
KW  - chromatin
KW  - priming
Y1  - 2019
U6  - https://doi.org/10.1111/pce.13365
VL  - 42
SP  - 771
EP  - 781
ER  -