TY  - GEN
A1  - Prát, Tomáš
A1  - Hajny ́, Jakub
A1  - Grunewald, Wim
A1  - Vasileva, Mina
A1  - Molnár, Gergely
A1  - Tejos, Ricardo
A1  - Schmid, Markus
A1  - Sauer, Michael
A1  - Friml, Jiří
T1  - WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17-and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain-and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1123 
KW  - apical-basal axis
KW  - arabidopsis-thaliana
KW  - root gravitropism
KW  - DNA-binding
KW  - gene-expression
KW  - transport
KW  - efflux
KW  - canalization
KW  - plants
KW  - phosphorylation
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-446331
SN  - 1866-8372
IS  - 1123
ER  - 
TY  - GEN
A1  - Powell, Anahid E.
A1  - Lenhard, Michael
T1  - Control of organ size in plants
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - The size of plant organs, such as leaves and flowers, is determined by an interaction of genotype and environmental influences. Organ growth occurs through the two successive processes of cell proliferation followed by cell expansion. A number of genes influencing either or both of these processes and thus contributing to the control of final organ size have been identified in the last decade. Although the overall picture of the genetic regulation of organ size remains fragmentary, two transcription factor/microRNA-based genetic pathways are emerging in the control of cell proliferation. However, despite this progress, fundamental questions remain unanswered, such as the problem of how the size of a growing organ could be monitored to determine the appropriate time for terminating growth. While genetic analysis will undoubtedly continue to advance our knowledge about size control in plants, a deeper understanding of this and other basic questions will require including advanced live-imaging and mathematical modeling, as impressively demonstrated by some recent examples. This should ultimately allow the comparison of the mechanisms underlying size control in plants and in animals to extract common principles and lineage-specific solutions.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 898 
KW  - BHLH transcription factor
KW  - genome-wide association
KW  - arabidopsis-thaliana
KW  - cell-proliferation
KW  - leaf development
KW  - developing leaves
KW  - petal growth
KW  - gene family
KW  - tor kinase
KW  - auxin
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438029
SN  - 1866-8372
IS  - 898
ER  - 
TY  - GEN
A1  - Lenhard, Michael
T1  - All's well that ends well
BT  - arresting cell proliferation in leaves
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The transition from cell proliferation to cell expansion is critical for determining leaf size. Andriankaja et al. (2012) demonstrate that in leaves of dicotyledonous plants, a basal proliferation zone is maintained for several days before abruptly disappearing, and that chloroplast differentiation is required to trigger the onset of cell expansion.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 906 
KW  - arabidopsis-thaliana
KW  - genetic-control
KW  - growth
KW  - size
KW  - curvature
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438035
SN  - 1866-8372
IS  - 906
SP  - 9
EP  - 11
ER  -