TY  - JOUR
A1  - Nawaz, Shiza
A1  - Khan, Muhammad Moman
A1  - Noack, Jonas
A1  - Awan, Asad Bashir
A1  - Schiebel, Juliane
A1  - Roggenbuck, Dirk
A1  - Schierack, Peter
A1  - Sarwar, Yasra
A1  - Ali, Aamir
T1  - Rapid detection of biofilm formation by zoonotic serovars of Salmonella enterica and avian pathogenic E. coli isolates from poultry
JF  - Pakistan veterinary journal
N2  - Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37 degrees C and 40 degrees C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40 degrees C and Rappaport-Vassiliadis Soya broth at 37 degrees C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control. (C) 2020 PVJ. All rights reserved
KW  - APEC
KW  - biofilm formation
KW  - Salmonella
KW  - video scan technology
Y1  - 2020
U6  - https://doi.org/10.29261/pakvetj/2020.066
SN  - 0253-8318
SN  - 2074-7764
VL  - 40
IS  - 4
SP  - 527
EP  - 530
PB  - University of Agriculture, Faculty of Veterinary Science
CY  - Faisalabad
ER  - 
TY  - JOUR
A1  - Awan, Asad Bashir
A1  - Schiebel, Juliane
A1  - Boehm, Alexander
A1  - Nitschke, Joerg
A1  - Sarwar, Yasra
A1  - Schierack, Peter
A1  - Ali, Aamir
T1  - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of pseudomonas  aeruginosa
JF  - EXCLI Journal
N2  - Multidrug resistant (MDR) Pseudomonas aeruginosa having strong biofilm potential and virulence factors are a serious threat for hospitalized patients having compromised immunity In this study, 34 P. aeruginosa isolates of human origin (17 MDR and 17 non-MDR clinical isolates) were checked for biofilm formation potential in enriched and minimal media. The biofilms were detected using crystal violet method and a modified software package of the automated VideoScan screening method. Cytotoxic potential of the isolates was also investigated on HepG2, LoVo and T24 cell lines using automated VideoScan technology. Pulse field gel electrophoresis revealed 10 PFGE types in MDR and 8 in non-MDR isolates. Although all isolates showed biofilm formation potential, strong biofilm formation was found more in enriched media than in minimal media. Eight MDR isolates showed strong biofilm potential in both enriched and minimal media by both detection methods. Strong direct correlation between crystal violet and VideoScan methods was observed in identifying strong biofilm forming isolates. High cytotoxic effect was observed by 4 isolates in all cell lines used while 6 other isolates showed high cytotoxic effect on T24 cell line only. Strong association of multidrug resistance was found with biofilm formation as strong biofilms were observed significantly higher in MDR isolates (p-value < 0.05) than non-MDR isolates. No significant association of cytotoxic potential with multidrug resistance or biofilm formation was found (p-value > 0.05). The MDR isolates showing significant cytotoxic effects and strong biofilm formation impose a serious threat for hospitalized patients with weak immune system.
KW  - Pseudomonas aeruginosa
KW  - multidrug resistance
KW  - biofilm
KW  - cytotoxicity
KW  - VideoScan technology
Y1  - 2019
U6  - https://doi.org/10.17179/excli2018-1948
SN  - 1611-2156
VL  - 18
SP  - 79
EP  - 90
PB  - Leibniz Research Centre for Working Environment and Human Factors
CY  - Dortmund
ER  -