TY  - JOUR
A1  - Stobiecki, Maciej
A1  - Skirycz, Aleksandra
A1  - Kerhoas, L.
A1  - Kachlicki, P.
A1  - Muth, D.
A1  - Einhorn, J.
A1  - Mueller-Roeber, Bernd
T1  - Profiling of phenolic glycosidic conjugates in leaves of Arabidopsis thaliana using LC/MS
JF  - Metabolomics : the official journal of the Metabolomics Society
N2  - Profiling of plant secondary metabolites is still a very difficult task. Liquid chromatography (LC) or capillary electrophoresis hyphenated with different kinds of detectors are methods of choice for analysis of polar, thermo labile compounds with high molecular masses. We demonstrate the applicability of LC combined with UV diode array or/and mass spectrometric detectors for the unambiguous identification and quantification of flavonoid conjugates isolated from Arahidopsis thaliana leaves of different genotypes and grown in different environmental conditions. During LC/UV/MS/MS analyses we were able to identify tetra-, tri, and di-glycosides of kaempferol, quercetin and isorhamnetin. Based on our results we can conclude that due to the co-elution of different chemical compounds in reversed phase H PLC systems the application of UV detectors does not allow to precisely profile all flavonoid conjugates existing in A. thaliana genotypes. Using MS detection it was possible to unambiguously recognize the glycosylation patterns of the aglycones. However, from the mass spectra we could not conclude neither the anomeric form of the C-1 carbon atoms of sugar moieties in glycosidic bonds between sugars or sugar and aglycone nor the position of the second carbon involved in disaccharides. The applicability of collision induced dissociation techniques (CID MS/MS) for structural analyses of the studied group of plant secondary metabolites with two types of analyzers (triple quadrupole or ion trap) was demonstrated.
KW  - liquid chromatography-mass spectrometry
KW  - metabolite profiling
KW  - metabolomics
KW  - flavonoid glycosides
Y1  - 2006
U6  - https://doi.org/10.1007/s11306-006-0031-5
SN  - 1573-3882
VL  - 2
SP  - 197
EP  - 219
PB  - Springer
CY  - New York
ER  -