TY  - JOUR
A1  - Heinsohn, Natascha Katharina
A1  - Niedl, Robert Raimund
A1  - Anielski, Alexander
A1  - Lisdat, Fred
A1  - Beta, Carsten
T1  - Electrophoretic mu PAD for purification and analysis of DNA samples
JF  - Biosensors : open access journal
N2  - In this work, the fabrication and characterization of a simple, inexpensive, and effective microfluidic paper analytic device (mu PAD) for monitoring DNA samples is reported. The glass microfiber-based chip has been fabricated by a new wax-based transfer-printing technique and an electrode printing process. It is capable of moving DNA effectively in a time-dependent fashion. The nucleic acid sample is not damaged by this process and is accumulated in front of the anode, but not directly on the electrode. Thus, further DNA processing is feasible. The system allows the DNA to be purified by separating it from other components in sample mixtures such as proteins. Furthermore, it is demonstrated that DNA can be moved through several layers of the glass fiber material. This proof of concept will provide the basis for the development of rapid test systems, e.g., for the detection of pathogens in water samples.
KW  - microfluidic paper analytic device (mu PAD)
KW  - patterning glass microfiber
KW  - fiber-electrophoresis chip
KW  - DNA
KW  - imprinted electrodes
KW  - cross layer chip
KW  - polymerase chain reaction (PCR)
KW  - purification
Y1  - 2022
U6  - https://doi.org/10.3390/bios12020062
SN  - 2079-6374
VL  - 12
IS  - 2
PB  - MDPI
CY  - Basel
ER  -