TY - JOUR A1 - Kaboth-Bahr, Stefanie A1 - Bahr, André A1 - Zeeden, Christian A1 - Yamoah, Kweku A. A1 - Lone, Mahjoor Ahmad A1 - Chuang, Chih-Kai A1 - Löwemark, Ludvig A1 - Wei, Kuo-Yen T1 - A tale of shifting relations BT - East Asian summer and winter monsoon variability during the Holocene JF - Scientific Reports N2 - Understanding the dynamics between the East Asian summer (EASM) and winter monsoon (EAWM) is needed to predict their variability under future global warming scenarios. Here, we investigate the relationship between EASM and EAWM as well as the mechanisms driving their variability during the last 10,000 years by stacking marine and terrestrial (non-speleothem) proxy records from the East Asian realm. This provides a regional and proxy independent signal for both monsoonal systems. The respective signal was subsequently analysed using a linear regression model. We find that the phase relationship between EASM and EAWM is not time-constant and significantly depends on orbital configuration changes. In addition, changes in the Atlantic Meridional Overturning circulation, Arctic sea-ice coverage, El Niño-Southern Oscillation and Sun Spot numbers contributed to millennial scale changes in the EASM and EAWM during the Holocene. We also argue that the bulk signal of monsoonal activity captured by the stacked non-speleothem proxy records supports the previously argued bias of speleothem climatic archives to moisture source changes and/or seasonality. KW - Environmental sciences KW - Ocean sciences KW - Solid Earth sciences Y1 - 2020 U6 - https://doi.org/10.1038/s41598-021-85444-7 SN - 2045-2322 VL - 11 PB - Macmillan Publishers Limited, part of Springer Nature CY - London ER - TY - JOUR A1 - Thapa, Samudrajit A1 - Wyłomańska, Agnieszka A1 - Sikora, Grzegorz A1 - Wagner, Caroline E. A1 - Krapf, Diego A1 - Kantz, Holger A1 - Chechkin, Aleksei V. A1 - Metzler, Ralf T1 - Leveraging large-deviation statistics to decipher the stochastic properties of measured trajectories JF - New Journal of Physics N2 - Extensive time-series encoding the position of particles such as viruses, vesicles, or individualproteins are routinely garnered insingle-particle tracking experiments or supercomputing studies.They contain vital clues on how viruses spread or drugs may be delivered in biological cells.Similar time-series are being recorded of stock values in financial markets and of climate data.Such time-series are most typically evaluated in terms of time-averaged mean-squareddisplacements (TAMSDs), which remain random variables for finite measurement times. Theirstatistical properties are different for differentphysical stochastic processes, thus allowing us toextract valuable information on the stochastic process itself. To exploit the full potential of thestatistical information encoded in measured time-series we here propose an easy-to-implementand computationally inexpensive new methodology, based on deviations of the TAMSD from itsensemble average counterpart. Specifically, we use the upper bound of these deviations forBrownian motion (BM) to check the applicability of this approach to simulated and real data sets.By comparing the probability of deviations fordifferent data sets, we demonstrate how thetheoretical bound for BM reveals additional information about observed stochastic processes. Weapply the large-deviation method to data sets of tracer beads tracked in aqueous solution, tracerbeads measured in mucin hydrogels, and of geographic surface temperature anomalies. Ouranalysis shows how the large-deviation properties can be efficiently used as a simple yet effectiveroutine test to reject the BM hypothesis and unveil relevant information on statistical propertiessuch as ergodicity breaking and short-time correlations. KW - diffusion KW - anomalous diffusion KW - large-deviation statistic KW - time-averaged mean squared displacement KW - Chebyshev inequality Y1 - 2020 U6 - https://doi.org/10.1088/1367-2630/abd50e SN - 1367-2630 VL - 23 PB - Dt. Physikalische Ges. ; IOP CY - Bad Honnef ; London ER - TY - JOUR A1 - Felser, Claudia A1 - Jessen, Anna T1 - Correlative coordination and variable subject-verb agreement in German JF - Languages : open access journal N2 - Coordinated subjects often show variable number agreement with the finite verb, but linguistic approaches to this phenomenon have rarely been informed by systematically collected data. We report the results from three experiments investigating German speakers' agreement preferences with complex subjects joined by the correlative conjunctions sowohl horizontal ellipsis als auch ('both horizontal ellipsis and'), weder horizontal ellipsis noch ('neither horizontal ellipsis nor') or entweder horizontal ellipsis oder ('either horizontal ellipsis or'). We examine to what extent conjunction type and a conjunct's relative proximity to the verb affect the acceptability and processibility of singular vs. plural agreement. Experiment 1 was an untimed acceptability rating task, Experiment 2 a timed sentence completion task, and Experiment 3 was a self-paced reading task. Taken together, our results show that number agreement with correlative coordination in German is primarily determined by a default constraint triggering plural agreement, which interacts with linear order and semantic factors. Semantic differences between conjunctions only affected speakers' agreement preferences in the absence of processing pressure but not their initial agreement computation. The combined results from our offline and online experimental measures of German speakers' agreement preferences suggest that the constraints under investigation do not only differ in their relative weighting but also in their relative timing during agreement computation. KW - correlative coordination KW - subject– verb agreement KW - German Y1 - 2021 U6 - https://doi.org/10.3390/languages6020067 SN - 2226-471X VL - 6 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Kühne, Franziska A1 - Paunov, Tatjana A1 - Weck, Florian T1 - Recognizing obsessive-compulsive disorder BT - How suitable is the German Zohar-Fineberg obsessive-compulsive screen? JF - BMC psychiatry N2 - Background Despite the prevalence of obsessive-compulsive disorder (OCD), its precise identification remains challenging. With the Zohar-Fineberg Obsessive-Compulsive Screen (ZF-OCS; 5 or 6 items), a brief instrument is widely available mainly in English. As there is a lack of empirical studies on the ZF-OCS, the aim of the present study was to translate the items into German and investigate the instrument in a nonclinical sample. Methods In two consecutive online surveys, n = 304 and n = 51 students participated. Besides the ZF-OCS, they answered established measures on OCD, depression, health anxiety, general anxiety and health-related well-being. Results Whereas internal consistency was low (α = .53–.72; ω = .55–.69), retest reliability (rt1,t2 = .89) at two weeks was high. As expected, we found high correlations with other OCD instruments (r > .61; convergent validity), and significantly weaker correlations with measures of depression (r = .39), health anxiety (r = .29), and health-related well-being (r = −.28, divergent validity). Nonetheless, the correlations with general anxiety were somewhere in between (r = .52). Conclusions Due to heterogeneous OCD subtypes, the ZF-OCS asks diverse questions which probably resulted in the present internal consistency. Nevertheless, the results on retest reliability and validity were promising. As for other OCD instruments, divergent validity regarding general anxiety seems problematic to establish. Even so, the ZF-OCS seems valuable for screening purposes, as it is short and easy to administer, and may facilitate initiating subsequent clinical assessment. Further studies should determine the instrument’s diagnostic accuracy. KW - Obsessive-compulsive disorder KW - Psychodiagnostics KW - Psychometric properties KW - Screening Y1 - 2021 U6 - https://doi.org/10.1186/s12888-021-03458-x SN - 1471-244X VL - 21 PB - Springer Nature CY - London ER - TY - JOUR A1 - Kühne, Franziska A1 - Fauth, Henriette A1 - Destina Sevde, Ay-Bryson A1 - Visser, Leonie N.C. A1 - Weck, Florian T1 - Communicating the diagnosis of cancer or depression: Results of a randomized controlled online study using video vignettes JF - Cancer Medicine N2 - Background Communicating a diagnosis is highly important, yet complex, especially in the context of cancer and mental disorders. The aim was to explore the communication style of an oncologist vs. psychotherapist in an online study. Methods Patients (N = 136: 65 cancer, 71 depression) were randomly assigned to watch a standardized video vignette with one of two communication styles (empathic vs. unempathic). Outcome measures of affectivity, information recall, communication skills, empathy and trust were applied. Results Regardless of diagnosis, empathic communication was associated with the perception of a significantly more empathic (p < 0.001, η2partial = 0.08) and trustworthy practitioner (p = 0.014, η2partial = 0.04) with better communication skills (p = 0.013, η2partial = 0.05). Cancer patients reported a larger decrease in positive affect (p < 0.001, η2partial = 0.15) and a larger increase in negative affect (p < 0.001, η2partial = 0.14) from pre- to post-video than depressive patients. Highly relevant information was recalled better in both groups (p < 0.001, d = 0.61–1.06). Conclusions The results highlight the importance of empathy while communicating both a diagnosis of cancer and a mental disorder. Further research should focus on the communication of a mental disorder in association with cancer. KW - consultation KW - mental health KW - oncology KW - psycho-oncology KW - skills Y1 - 2021 U6 - https://doi.org/10.1002/cam4.4396 SN - 2045-7634 VL - 10 SP - 9012 EP - 9021 PB - Wiley CY - Hoboken, New Jersey, USA ET - 24 ER - TY - JOUR A1 - Wojcik, Laurie Anne A1 - Ceulemans, Ruben A1 - Gaedke, Ursula T1 - Functional diversity buffers the effects of a pulse perturbation on the dynamics of tritrophic food webs JF - Ecology and Evolution N2 - Biodiversity decline causes a loss of functional diversity, which threatens ecosystems through a dangerous feedback loop: This loss may hamper ecosystems’ ability to buffer environmental changes, leading to further biodiversity losses. In this context, the increasing frequency of human-induced excessive loading of nutrients causes major problems in aquatic systems. Previous studies investigating how functional diversity influences the response of food webs to disturbances have mainly considered systems with at most two functionally diverse trophic levels. We investigated the effects of functional diversity on the robustness, that is, resistance, resilience, and elasticity, using a tritrophic—and thus more realistic—plankton food web model. We compared a non-adaptive food chain with no diversity within the individual trophic levels to a more diverse food web with three adaptive trophic levels. The species fitness differences were balanced through trade-offs between defense/growth rate for prey and selectivity/half-saturation constant for predators. We showed that the resistance, resilience, and elasticity of tritrophic food webs decreased with larger perturbation sizes and depended on the state of the system when the perturbation occurred. Importantly, we found that a more diverse food web was generally more resistant and resilient but its elasticity was context-dependent. Particularly, functional diversity reduced the probability of a regime shift toward a non-desirable alternative state. The basal-intermediate interaction consistently determined the robustness against a nutrient pulse despite the complex influence of the shape and type of the dynamical attractors. This relationship was strongly influenced by the diversity present and the third trophic level. Overall, using a food web model of realistic complexity, this study confirms the destructive potential of the positive feedback loop between biodiversity loss and robustness, by uncovering mechanisms leading to a decrease in resistance, resilience, and potentially elasticity as functional diversity declines. KW - functional diversity KW - nutrient spike KW - pulse perturbation KW - regime shift KW - robustness KW - tritrophic food web Y1 - 2021 U6 - https://doi.org/10.1002/ece3.8214 SN - 2045-7758 N1 - Wojcik and Ceulemans shared first authorship. VL - 11 IS - 22 SP - 15639 EP - 15663 PB - John Wiley & Sons, Inc. CY - Hoboken (New Jersey) ER - TY - JOUR A1 - Grubic, Mira A1 - Wierzba, Marta T1 - The German additive particle noch BT - testing the role of topic situations JF - Glossa : a journal of general linguistics N2 - The particle noch (‘still’) can have an additive reading similar to auch (‘also’). We argue that both particles indicate that a previously partially answered QUD is re-opened to add a further answer. The particles differ in that the QUD, in the case of auch, can be re-opened with respect to the same topic situation, whereas noch indicates that the QUD is re-opened with respect to a new topic situation. This account predicts a difference in the accommodation behavior of the two particles. We present an experiment whose results are in line with this prediction. KW - additive particles KW - noch KW - auch KW - German KW - topic situation KW - semantics KW - experiments Y1 - 2020 U6 - https://doi.org/10.5334/gjgl.1275 SN - 2397-1835 VL - 6 IS - 1 PB - Ubiquity Press CY - London ER - TY - JOUR A1 - Ouergui, Ibrahim A1 - Delleli, Slaheddine A1 - Bouassida, Anissa A1 - Bouhlel, Ezdine A1 - Chaabene, Helmi A1 - Ardigò, Luca Paolo A1 - Franchini, Emerson T1 - Technical-tactical analysis of small combat games in male kickboxers BT - Effects of varied number of opponents and area size JF - BMC Sports Science, Medicine and Rehabilitation N2 - Background: To handle the competition demands, sparring drills are used for specific technical–tactical training as well as physical–physiological conditioning in combat sports. While the effects of different area sizes and number of within-round sparring partners on physiological and perceptive responses in combats sports were examined in previous studies, technical and tactical aspects were not investigated. This study investigated the effect of different within-round sparring partners number (i.e., at a time; 1 vs. 1, 1 vs. 2, and 1 vs. 4) and area sizes (2 m × 2 m, 4 m × 4 m, and 6 m × 6 m) variation on the technical–tactical aspects of small combat games in kickboxing. Method: Twenty male kickboxers (mean ± standard deviation, age: 20.3 ± 0.9 years), regularly competing in regional and national events randomly performed nine different kickboxing combats, lasting 2 min each. All combats were video recorded and analyzed using the software Dartfish. Results: Results showed that the total number of punches was significantly higher in 1 versus 4 compared with 1 versus 1 (p = 0.011, d = 0.83). Further, the total number of kicks was significantly higher in 1 versus 4 compared with 1 versus 1 and 1 versus 2 (p < 0.001; d = 0.99 and d = 0.83, respectively). Moreover, the total number of kick combinations was significantly higher in 1 versus 4 compared with 1 versus 1 and 1 versus 2 (p < 0.001; d = 1.05 and d = 0.95, respectively). The same outcome was significantly lower in 2 m × 2 m compared with 4 m × 4 m and 6 m × 6 m areas (p = 0.010 and d = − 0.45; p < 0.001 and d = − 0.6, respectively). The number of block-and-parry was significantly higher in 1 versus 4 compared with 1 versus 1 (p < 0.001, d = 1.45) and 1 versus 2 (p = 0.046, d = 0.61) and in 2 m × 2 m compared with 4 m × 4 m and 6 × 6 m areas (p < 0.001; d = 0.47 and d = 0.66, respectively). Backwards lean actions occurred more often in 2 m × 2 m compared with 4 m × 4 m (p = 0.009, d = 0.53) and 6 m × 6 m (p = 0.003, d = 0.60). However, the number of foot defenses was significantly lower in 2 m × 2 m compared with 6 m × 6 m (p < 0.001, d = 1.04) and 4 m × 4 m (p = 0.004, d = 0.63). Additionally, the number of clinches was significantly higher in 1 versus 1 compared with 1 versus 2 (p = 0.002, d = 0.7) and 1 versus 4 (p = 0.034, d = 0.45). Conclusions: This study provides practical insights into how to manipulate within-round sparring partners’ number and/or area size to train specific kickboxing technical–tactical fundamentals. KW - Martial arts KW - Time-motion analysis KW - Punch KW - Kick KW - Defensive actions Y1 - 2021 U6 - https://doi.org/10.1186/s13102-021-00391-0 SN - 2052-1847 N1 - Luca Paolo Ardigò and Emerson Franchini have contributed equally to this work. IS - 13 PB - Springer Nature CY - Berlin ER - TY - JOUR A1 - Raju, Rajarshi Roy A1 - Koetz, Joachim T1 - Inner rotation of Pickering Janus emulsions JF - Nanomaterials : open access journal N2 - Janus droplets were prepared by vortex mixing of three non-mixable liquids, i.e., olive oil, silicone oil and water, in the presence of gold nanoparticles (AuNPs) in the aqueous phase and magnetite nanoparticles (MNPs) in the olive oil. The resulting Pickering emulsions were stabilized by a red-colored AuNP layer at the olive oil/water interface and MNPs at the oil/oil interface. The core–shell droplets can be stimulated by an external magnetic field. Surprisingly, an inner rotation of the silicon droplet is observed when MNPs are fixed at the inner silicon droplet interface. This is the first example of a controlled movement of the inner parts of complex double emulsions by magnetic manipulation via interfacially confined magnetic nanoparticles. KW - Janus droplets KW - Pickering emulsions KW - magnetic manipulation KW - gold nanoparticles KW - magnetite nanoparticles Y1 - 2021 U6 - https://doi.org/10.3390/nano11123312 SN - 2079-4991 VL - 11 IS - 12 PB - MDPI CY - Basel ER - TY - JOUR A1 - Baumgardt, Iris T1 - Berufliche Orientierung von Kindern im Grundschulalter BT - Analyse von ausgewählten Projekten, Unterrichtsmaterialien und Lehrplänen N2 - Die berufliche Orientierung von Kindern im Grundschulalter ist bislang nur in Ansätzen erforscht. Gleichwohl gibt es berufsorientierende Angebote, die auf verschiedenen Ebenen Grundschulkinder adressieren. Die Untersuchung fokussiert aktuelle Forschungsergebnisse, ausgewählte Initiativen, Kinderbücher, Unterrichtsmaterialien usw. zur beruflichen Orientierung von Kindern. Mit dem Ziel der Entwicklung und Ausdifferenzierung eines facettenreichen beruflichen Selbstkonzeptes von Kindern werden spezifische Forschungs- und Entwicklungspotenziale aufgezeigt. Y1 - 2022 SN - 978-3-8340-2199-1 SN - 978-3-7639-7188-6 U6 - https://doi.org/10.3278/9783763971886 PB - Schneider Verlag Hohengehren GmbH CY - Baltmannsweiler ER - TY - JOUR A1 - Rose, Robert A1 - Groeger, Lars A1 - Hölzle, Katharina T1 - The Emergence of Shared Leadership in Innovation Labs JF - Frontiers in Psychology N2 - Implementing innovation laboratories to leverage intrapreneurship are an increasingly popular organizational practice. A typical feature in these creative environments are semi-autonomous teams in which multiple members collectively exert leadership influence, thereby challenging traditional command-and-control conceptions of leadership. An extensive body of research on the team-centric concept of shared leadership has recognized the potential for pluralized leadership structures in enhancing team effectiveness; however, little empirical work has been conducted in organizational contexts in which creativity is key. This study set out to explore antecedents of shared leadership and its influence on team creativity in an innovation lab. Building on extant shared leadership and innovation research, we propose antecedents customary to creative teamwork, that is, experimental culture, task reflexivity, and voice. Multisource data were collected from 104 team members and 49 evaluations of 29 coaches nested in 21 teams working in a prototypical innovation lab. We identify factors specific to creative teamwork that facilitate the emergence of shared leadership by providing room for experimentation, encouraging team members to speak up in the creative process, and cultivating a reflective application of entrepreneurial thinking. We provide specific exemplary activities for innovation lab teams to increase levels of shared leadership. KW - innovation laboratories KW - intrapreneurship KW - team creativity KW - shared leadership KW - social network analysis Y1 - 2021 U6 - https://doi.org/10.3389/fpsyg.2021.685167 SN - 1664-1078 VL - 12 SP - 1 EP - 13 PB - Frontiers in psychology CY - Lausanne, Schweiz ER - TY - JOUR A1 - Romero-Mujalli, Daniel A1 - Rochow, Markus A1 - Kahl, Sandra M. A1 - Paraskevopoulou, Sofia A1 - Folkertsma, Remco A1 - Jeltsch, Florian A1 - Tiedemann, Ralph T1 - Adaptive and nonadaptive plasticity in changing environments: Implications for sexual species with different life history strategies JF - Ecology and Evolution N2 - Populations adapt to novel environmental conditions by genetic changes or phenotypic plasticity. Plastic responses are generally faster and can buffer fitness losses under variable conditions. Plasticity is typically modeled as random noise and linear reaction norms that assume simple one-to- one genotype–phenotype maps and no limits to the phenotypic response. Most studies on plasticity have focused on its effect on population viability. However, it is not clear, whether the advantage of plasticity depends solely on environmental fluctuations or also on the genetic and demographic properties (life histories) of populations. Here we present an individual-based model and study the relative importance of adaptive and nonadaptive plasticity for populations of sexual species with different life histories experiencing directional stochastic climate change. Environmental fluctuations were simulated using differentially autocorrelated climatic stochasticity or noise color, and scenarios of directiona climate change. Nonadaptive plasticity was simulated as a random environmental effect on trait development, while adaptive plasticity as a linear, saturating, or sinusoidal reaction norm. The last two imposed limits to the plastic response and emphasized flexible interactions of the genotype with the environment. Interestingly, this assumption led to (a) smaller phenotypic than genotypic variance in the population (many-to- one genotype–phenotype map) and the coexistence of polymorphisms, and (b) the maintenance of higher genetic variation—compared to linear reaction norms and genetic determinism—even when the population was exposed to a constant environment for several generations. Limits to plasticity led to genetic accommodation, when costs were negligible, and to the appearance of cryptic variation when limits were exceeded. We found that adaptive plasticity promoted population persistence under red environmental noise and was particularly important for life histories with low fecundity. Populations produing more offspring could cope with environmental fluctuations solely by genetic changes or random plasticity, unless environmental change was too fast. KW - developmental canalization KW - environmental change KW - genetic accommodation KW - Individual-based models KW - limits KW - many-to-one genotype–phenotype map KW - noise color KW - phenotypic plasticity KW - reaction norms KW - stochastic fluctuations Y1 - 2020 SN - 2045-7758 VL - 11 IS - 11 PB - John Wiley & Sons, Inc. CY - New Jersey ER - TY - JOUR A1 - Cahsan, Binia De A1 - Westbury, Michael V. A1 - Paraskevopoulou, Sofia A1 - Drews, Hauke A1 - Ott, Moritz A1 - Gollmann, Günter A1 - Tiedemann, Ralph T1 - Genomic consequences of human-mediated translocations in margin populations of an endangered amphibian JF - Evolutionary Applications N2 - Due to their isolated and often fragmented nature, range margin populations are especially vulnerable to rapid environmental change. To maintain genetic diversity and adaptive potential, gene flow from disjunct populations might therefore be crucial to their survival. Translocations are often proposed as a mitigation strategy to increase genetic diversity in threatened populations. However, this also includes the risk of losing locally adapted alleles through genetic swamping. Human-mediated translocations of southern lineage specimens into northern German populations of the endangered European fire-bellied toad (Bombina bombina) provide an unexpected experimental set-up to test the genetic consequences of an intraspecific introgression from central population individuals into populations at the species range margin. Here, we utilize complete mitochondrial genomes and transcriptome nuclear data to reveal the full genetic extent of this translocation and the consequences it may have for these populations. We uncover signs of introgression in four out of the five northern populations investigated, including a number of introgressed alleles ubiquitous in all recipient populations, suggesting a possible adaptive advantage. Introgressed alleles dominate at the MTCH2 locus, associated with obesity/fat tissue in humans, and the DSP locus, essential for the proper development of epidermal skin in amphibians. Furthermore, we found loci where local alleles were retained in the introgressed populations, suggesting their relevance for local adaptation. Finally, comparisons of genetic diversity between introgressed and nonintrogressed northern German populations revealed an increase in genetic diversity in all German individuals belonging to introgressed populations, supporting the idea of a beneficial transfer of genetic variation from Austria into North Germany. KW - adaptive introgression KW - admixture KW - Bombina bombina KW - genetic rescue KW - mitogenomes KW - transcriptomics Y1 - 2020 SN - 1752-4563 VL - 14 IS - 6 PB - John Wiley & Sons, Inc. CY - New Jersey ER - TY - JOUR A1 - Spikes, Montrai A1 - Rodríguez-Silva, Rodet A1 - Bennett, Kerri-Ann A1 - Bräger, Stefan A1 - Josaphat, James A1 - Torres-Pineda, Patricia A1 - Ernst, Anja A1 - Havenstein, Katja A1 - Schlupp, Ingo A1 - Tiedemann, Ralph T1 - A phylogeny of the genus Limia (Teleostei: Poeciliidae) suggests a single-lake radiation nested in a Caribbean-wide allopatric speciation scenario JF - BMC Research Notes N2 - Objective The Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genus Limia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of the Limia species in Lake Miragoâne, relative to their congeners throughout the Caribbean. Results For 12 Limia species, we obtained almost complete sequences of the mitochondrial cytochrome b gene, a well-established marker for lower-level taxonomic relationships. We included sequences of six further Limia species from GenBank (total N  = 18 species). Our phylogenies are in concordance with other published phylogenies of Limia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade. KW - Cytochrome b KW - Island biogeography KW - Fresh water fish KW - Phylogeny Y1 - 2021 U6 - https://doi.org/10.1186/s13104-021-05843-x SN - 1756-0500 VL - 14 SP - 1 EP - 8 PB - BMC Research Notes / Biomed Central CY - London ER - TY - JOUR A1 - Krüger, Johanna A1 - Foerster, Verena Elisabeth A1 - Trauth, Martin H. A1 - Hofreiter, Michael A1 - Tiedemann, Ralph T1 - Exploring the Past Biosphere of Chew Bahir/Southern Ethiopia: Cross-Species Hybridization Capture of Ancient Sedimentary DNA from a Deep Drill Core JF - Frontiers in Earth Science N2 - Eastern Africa has been a prime target for scientific drilling because it is rich in key paleoanthropological sites as well as in paleolakes, containing valuable paleoclimatic information on evolutionary time scales. The Hominin Sites and Paleolakes Drilling Project (HSPDP) explores these paleolakes with the aim of reconstructing environmental conditions around critical episodes of hominin evolution. Identification of biological taxa based on their sedimentary ancient DNA (sedaDNA) traces can contribute to understand past ecological and climatological conditions of the living environment of our ancestors. However, sedaDNA recovery from tropical environments is challenging because high temperatures, UV irradiation, and desiccation result in highly degraded DNA. Consequently, most of the DNA fragments in tropical sediments are too short for PCR amplification. We analyzed sedaDNA in the upper 70 m of the composite sediment core of the HSPDP drill site at Chew Bahir for eukaryotic remnants. We first tested shotgun high throughput sequencing which leads to metagenomes dominated by bacterial DNA of the deep biosphere, while only a small fraction was derived from eukaryotic, and thus probably ancient, DNA. Subsequently, we performed cross-species hybridization capture of sedaDNA to enrich ancient DNA (aDNA) from eukaryotic remnants for paleoenvironmental analysis, using established barcoding genes (cox1 and rbcL for animals and plants, respectively) from 199 species that may have had relatives in the past biosphere at Chew Bahir. Metagenomes yielded after hybridization capture are richer in reads with similarity to cox1 and rbcL in comparison to metagenomes without prior hybridization capture. Taxonomic assignments of the reads from these hybridization capture metagenomes also yielded larger fractions of the eukaryotic domain. For reads assigned to cox1, inferred wet periods were associated with high inferred relative abundances of putative limnic organisms (gastropods, green algae), while inferred dry periods showed increased relative abundances for insects. These findings indicate that cross-species hybridization capture can be an effective approach to enhance the information content of sedaDNA in order to explore biosphere changes associated with past environmental conditions, enabling such analyses even under tropical conditions. KW - Chew Bahir KW - hybridization capture KW - ICDP KW - paleoclimate KW - past biosphere KW - sedaDNA KW - sediment core Y1 - 2021 U6 - https://doi.org/10.3389/feart.2021.683010 SN - 2296-6463 SP - 1 EP - 20 PB - Frontiers in Earth Science CY - Lausanne, Schweiz ER - TY - JOUR A1 - Feil, Hauke T1 - The cancer of corruption and World Bank project performance BT - Is there a connection? JF - Development policy review / publ. for the Overseas Development Institute N2 - Motivation: Corruption is often cited as a central reason why development projects fail. The article tests this claim by assessing whether World Bank projects perform worse in implementation environments with a higher corruption level. The article focuses specifically on bribery between public officials and firms during the procurement of needed goods and services. Approach and Methods: I use data from the World Bank's Enterprise Surveys to avoid the often-criticized corruption perception indices and to allow for an assessment of effects at the subnational level. The analysis builds on an assessment of the performance ratings of 1,228 World Bank projects and covers 87 different countries. Finding: Overall, the article finds a small but statistically significant correlation between the corruption level and project performance. This result indicates that the corruption level of recipient countries should be considered during the design and implementation of projects. Policy Implications: Nonetheless, the relatively small correlation and the low pseudo R-squareds advise not overestimating the relevance of corruption for project performance. At least for the project level, the article finds no indication that corruption is a primary obstacle to aid effectiveness. KW - Aid effectiveness KW - corruption KW - Enterprise Survey KW - project performance KW - World Bank Y1 - 2020 U6 - https://doi.org/10.1111/dpr.12503 SN - 1467-7679 SN - 0950-6764 VL - 39 IS - 3 SP - 381 EP - 397 PB - Blackwell Publ. CY - Oxford ER - TY - JOUR A1 - Tjaden, Jasper T1 - Measuring migration 2.0 BT - a review of digital data sources JF - Comparative migration studies : CMS N2 - The interest in human migration is at its all-time high, yet data to measure migration is notoriously limited. “Big data” or “digital trace data” have emerged as new sources of migration measurement complementing ‘traditional’ census, administrative and survey data. This paper reviews the strengths and weaknesses of eight novel, digital data sources along five domains: reliability, validity, scope, access and ethics. The review highlights the opportunities for migration scholars but also stresses the ethical and empirical challenges. This review intends to be of service to researchers and policy analysts alike and help them navigate this new and increasingly complex field. KW - Migration KW - Big data KW - Digital trace KW - Measurement KW - Survey KW - Review Y1 - 2021 U6 - https://doi.org/10.1186/s40878-021-00273-x SN - 2214-594X VL - 9 IS - 1 PB - Springer CY - London ER - TY - JOUR A1 - Tchewonpi Sagu, Sorel A1 - Landgräber, Eva A1 - Henkel, Ina M. A1 - Huschek, Gerd A1 - Homann, Thomas A1 - Bußler, Sara A1 - Schlüter, Oliver K. A1 - Rawel, Harshadrai Manilal T1 - Effect of cereal α-amylase/trypsin inhibitors on developmental characteristics and abundance of digestive enzymes of mealworm larvae (Tenebrio molitor L.) JF - Insects N2 - The objective of this work was to investigate the potential effect of cereal α-amylase/trypsin inhibitors (ATIs) on growth parameters and selective digestive enzymes of Tenebrio molitor L. larvae. The approach consisted of feeding the larvae with wheat, sorghum and rice meals containing different levels and composition of α-amylase/trypsin inhibitors. The developmental and biochemical characteristics of the larvae were assessed over feeding periods of 5 h, 5 days and 10 days, and the relative abundance of α-amylase and selected proteases in larvae were determined using liquid chromatography tandem mass spectrometry. Overall, weight gains ranged from 21% to 42% after five days of feeding. The larval death rate significantly increased in all groups after 10 days of feeding (p < 0.05), whereas the pupation rate was about 25% among larvae fed with rice (Oryza sativa L.) and Siyazan/Esperya wheat meals, and only 8% and 14% among those fed with Damougari and S35 sorghum meals. As determined using the Lowry method, the protein contents of the sodium phosphate extracts ranged from 7.80 ± 0.09 to 9.42 ± 0.19 mg/mL and those of the ammonium bicarbonate/urea reached 19.78 ± 0.16 to 37.47 ± 1.38 mg/mL. The total protein contents of the larvae according to the Kjeldahl method ranged from 44.0 and 49.9 g/100 g. The relative abundance of α-amylase, CLIP domain-containing serine protease, modular serine protease zymogen and C1 family cathepsin significantly decreased in the larvae, whereas dipeptidylpeptidase I and chymotrypsin increased within the first hours after feeding (p < 0.05). Trypsin content was found to be constant independently of time or feed material. Finally, based on the results we obtained, it was difficult to substantively draw conclusions on the likely effects of meal ATI composition on larval developmental characteristics, but their effects on the digestive enzyme expression remain relevant. KW - growth behavior KW - Tenebrio molitor larvae KW - feeding KW - cereal meals KW - α-amylase/trypsin inhibitors KW - digestive enzymes quantification KW - LC-MS/MS Y1 - 2021 U6 - https://doi.org/10.3390/insects12050454 SN - 2075-4450 VL - 12 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Sagu Tchewonpi, Sorel A1 - Huschek, Gerd A1 - Homann, Thomas A1 - Rawel, Harshadrai Manilal T1 - Effect of sample preparation on the detection and quantification of selected nuts allergenic proteins by LC-MS/MS JF - Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International N2 - The detection and quantification of nut allergens remains a major challenge. The liquid chroma-tography tandem mass spectrometry (LC-MS/MS) is emerging as one of the most widely used methods, but sample preparation prior to the analysis is still a key issue. The objective of this work was to establish optimized protocols for extraction, tryptic digestion and LC-MS analysis of almond, cashew, hazelnut, peanut, pistachio and walnut samples. Ammonium bicar-bonate/urea extraction (Ambi/urea), SDS buffer extraction (SDS), polyvinylpolypyrroli-done (PVPP) extraction, trichloroacetic acid/acetone extraction (TCA/acetone) and chloro-form/methanol/sodium chloride precipitation (CM/NaCl) as well as the performances of con-ventional tryptic digestion and microwave-assisted breakdown were investigated. Overall, the protein extraction yields ranged from 14.9 ± 0.5 (almond extract from CM/NaCl) to 76.5 ± 1.3% (hazelnut extract from Ambi/urea). Electrophoretic profiling showed that the SDS extraction method clearly presented a high amount of extracted proteins in the range of 0–15 kDa, 15–35 kDa, 35–70 kDa and 70–250 kDa compared to the other methods. The linearity of the LC-MS methods in the range of 0 to 0.4 µg equivalent defatted nut flour was assessed and recovery of internal standards GWGG and DPLNV(d8)LKPR ranged from 80 to 120%. The identified bi-omarkers peptides were used to relatively quantifier selected allergenic protein form the inves-tigated nut samples. Considering the overall results, it can be concluded that SDS buffer allows a better protein extraction from almond, peanut and walnut samples while PVPP buffer is more appropriate for cashew, pistachio and hazelnut samples. It was also found that conventional overnight digestion is indicated for cashew, pistachio and hazelnut samples, while microwave assisted tryptic digestion is recommended for almond, hazelnut and peanut extracts. KW - nut allergenic proteins KW - protein extraction KW - sample preparation KW - tryptic digestion KW - microwave assisted digestion KW - SDS PAGE KW - LC-MS/MS Y1 - 2021 U6 - https://doi.org/10.3390/molecules26154698 SN - 1420-3049 VL - 26 IS - 15 PB - MDPI CY - Basel ER - TY - JOUR A1 - Gräf, Ralph A1 - Grafe, Marianne A1 - Meyer, Irene A1 - Mitic, Kristina A1 - Pitzen, Valentin T1 - The dictyostelium centrosome JF - Cells : open access journal N2 - The centrosome of Dictyostelium amoebae contains no centrioles and consists of a cylindrical layered core structure surrounded by a corona harboring microtubule-nucleating gamma-tubulin complexes. It is the major centrosomal model beyond animals and yeasts. Proteomics, protein interaction studies by BioID and superresolution microscopy methods led to considerable progress in our understanding of the composition, structure and function of this centrosome type. We discuss all currently known components of the Dictyostelium centrosome in comparison to other centrosomes of animals and yeasts. KW - microtubule-organizing center KW - microtubule-organization KW - centrosome KW - Dictyostelium KW - mitosis Y1 - 2021 U6 - https://doi.org/10.3390/cells10102657 SN - 2073-4409 VL - 10 IS - 10 PB - MDPI CY - Basel ER -