TY  - JOUR
A1  - Ferrari, Camilla
A1  - Proost, Sebastian
A1  - Janowski, Marcin Andrzej
A1  - Becker, Jörg
A1  - Nikoloski, Zoran
A1  - Bhattacharya, Debashish
A1  - Price, Dana
A1  - Tohge, Takayuki
A1  - Bar-Even, Arren
A1  - Fernie, Alisdair R.
A1  - Stitt, Mark
A1  - Mutwil, Marek
T1  - Kingdom-wide comparison reveals the evolution of diurnal gene expression in Archaeplastida
JF  - Nature Communications
N2  - Plants have adapted to the diurnal light-dark cycle by establishing elaborate transcriptional programs that coordinate many metabolic, physiological, and developmental responses to the external environment. These transcriptional programs have been studied in only a few species, and their function and conservation across algae and plants is currently unknown. We performed a comparative transcriptome analysis of the diurnal cycle of nine members of Archaeplastida, and we observed that, despite large phylogenetic distances and dramatic differences in morphology and lifestyle, diurnal transcriptional programs of these organisms are similar. Expression of genes related to cell division and the majority of biological pathways depends on the time of day in unicellular algae but we did not observe such patterns at the tissue level in multicellular land plants. Hence, our study provides evidence for the universality of diurnal gene expression and elucidates its evolutionary history among different photosynthetic eukaryotes.
Y1  - 2019
U6  - https://doi.org/10.1038/s41467-019-08703-2
SN  - 2041-1723
VL  - 10
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Küken, Anika
A1  - Sommer, Frederik
A1  - Yaneva-Roder, Liliya
A1  - Mackinder, Luke C. M.
A1  - Hoehne, Melanie
A1  - Geimer, Stefan
A1  - Jonikas, Martin C.
A1  - Schroda, Michael
A1  - Stitt, Mark
A1  - Nikoloski, Zoran
A1  - Mettler-Altmann, Tabea
T1  - Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts
JF  - eLife
N2  - Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.
Y1  - 2018
U6  - https://doi.org/10.7554/eLife.37960
SN  - 2050-084X
VL  - 7
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -