TY - JOUR A1 - Pourteau, Amaury A1 - Scherer, Erik E. A1 - Schorn, Simon A1 - Bast, Rebecca A1 - Schmidt, Alexander A1 - Ebert, Lisa T1 - Thermal evolution of an ancient subduction interface revealed by Lu-Hf garnet geochronology, Halilbagi Complex (Anatolia) JF - Geoscience Frontiers N2 - The thermal structure of subduction zones exerts a major influence on deep-seated mechanical and chemical processes controlling arc magmatism, seismicity, and global element cycles. Accretionary complexes exposed inland may comprise tectonic blocks with contrasting pressure-temperature (P-T) histories, making it possible to investigate the dynamics and thermal evolution of former subduction interfaces. With this aim, we present new Lu-Hf geochronological results for mafic rocks of the Halilbagi Complex (Anatolia) that evolved along different thermal gradients. Samples include a lawsonite-epidote blueschist, a lawsonite-epidote eclogite, and an epidote eclogite (all with counter-clockwise P-T paths), a prograde lawsonite blueschist with a "hairpin"-type P-T path, and a garnet amphibolite from the overlying sub-ophiolitic metamorphic sole. Equilibrium phase diagrams suggest that the garnet amphibolite formed at similar to 0.6-0.7 GPa and 800-850 degrees C, whereas the prograde lawsonite blueschist records burial from 2.1 GPa and 420 degrees C to 2.6 GPa and 520 degrees C. Well-defined Lu-Hf isochrons were obtained for the epidote eclogite (92.38 +/- 0.22 Ma) and the lawsonite-epidote blueschist (90.19 +/- 0.54 Ma), suggesting rapid garnet growth. The lawsonite-epidote eclogite (87.30 +/- 0.39 Ma) and the prograde lawsonite blueschist (ca. 86 Ma) are younger, whereas the garnet amphibolite (104.5 +/- 3.5 Ma) is older. Our data reveal a consistent trend of progressively decreasing geothermal gradient from granulite-facies conditions at similar to 104 Ma to the epidote-eclogite facies around 92 Ma, and the lawsonite blueschist-facies between 90 Ma and 86 Ma. Three Lu-Hf garnet dates (between 92 Ma and 87 Ma) weighted toward the growth of post-peak rims (as indicated by Lu distribution in garnet) suggest that the HP/LT rocks were exhumed continuously and not episodically. We infer that HP/LT metamorphic rocks within the Halilbagi Complex were subjected to continuous return flow, with "warm" rocks being exhumed during the tectonic burial of "cold" ones. Our results, combined with regional geological constraints, allow us to speculate that subduction started at a transform fault near a mid-oceanic spreading centre. Following its formation, this ancient subduction interface evolved thermally over more than 15 Myr, most likely as a result of heat dissipation rather than crustal underplating. (C) 2018, China University of Geosciences (Beijing) and Peking University. Production and hosting by Elsevier B.V. KW - Subduction KW - Lu/Hf dating of garnet KW - Metamorphic sole KW - Eclogite KW - Blueschist KW - Lawsonite Y1 - 2019 U6 - https://doi.org/10.1016/j.gsf.2018.03.004 SN - 1674-9871 VL - 10 IS - 1 SP - 127 EP - 148 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Foster, William J. A1 - Heindel, Katrin A1 - Richoz, Sylvain A1 - Gliwa, Jana A1 - Lehrmann, Daniel J. A1 - Baud, Aymon A1 - Kolar-Jurkovsek, Tea A1 - Aljinovic, Dunja A1 - Jurkovsek, Bogdan A1 - Korn, Dieter A1 - Martindale, Rowan C. A1 - Peckmann, Jörn T1 - Suppressed competitive exclusion enabled the proliferation of Permian/Triassic boundary microbialites JF - The Depositional Record : the open access journal of the International Association of Sedimentologists N2 - During the earliest Triassic microbial mats flourished in the photic zones of marginal seas, generating widespread microbialites. It has been suggested that anoxic conditions in shallow marine environments, linked to the end-Permian mass extinction, limited mat-inhibiting metazoans allowing for this microbialite expansion. The presence of a diverse suite of proxies indicating oxygenated shallow sea-water conditions (metazoan fossils, biomarkers and redox proxies) from microbialite successions have, however, challenged the inference of anoxic conditions. Here, the distribution and faunal composition of Griesbachian microbialites from China, Iran, Turkey, Armenia, Slovenia and Hungary are investigated to determine the factors that allowed microbialite-forming microbial mats to flourish following the end-Permian crisis. The results presented here show that Neotethyan microbial buildups record a unique faunal association due to the presence of keratose sponges, while the Palaeotethyan buildups have a higher proportion of molluscs and the foraminifera Earlandia. The distribution of the faunal components within the microbial fabrics suggests that, except for the keratose sponges and some microconchids, most of the metazoans were transported into the microbial framework via wave currents. The presence of both microbialites and metazoan associations were limited to oxygenated settings, suggesting that a factor other than anoxia resulted in a relaxation of ecological constraints following the mass extinction event. It is inferred that the end-Permian mass extinction event decreased the diversity and abundance of metazoans to the point of significantly reducing competition, allowing photosynthesis-based microbial mats to flourish in shallow water settings and resulting in the formation of widespread microbialites. KW - Competitive exclusion KW - Permian KW - Triassic KW - mass extinction KW - microbialites KW - palaeoecology Y1 - 2019 U6 - https://doi.org/10.1002/dep2.97 SN - 2055-4877 VL - 6 IS - 1 SP - 62 EP - 74 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Wendler, Petra A1 - Enenkel, Cordula T1 - Nuclear Transport of Yeast Proteasomes JF - Frontiers in molecular biosciences N2 - Proteasomes are key proteases in regulating protein homeostasis. Their holo-enzymes are composed of 40 different subunits which are arranged in a proteolytic core (CP) flanked by one to two regulatory particles (RP). Proteasomal proteolysis is essential for the degradation of proteins which control time-sensitive processes like cell cycle progression and stress response. In dividing yeast and human cells, proteasomes are primarily nuclear suggesting that proteasomal proteolysis is mainly required in the nucleus during cell proliferation. In yeast, which have a closed mitosis, proteasomes are imported into the nucleus as immature precursors via the classical import pathway. During quiescence, the reversible absence of proliferation induced by nutrient depletion or growth factor deprivation, proteasomes move from the nucleus into the cytoplasm. In the cytoplasm of quiescent yeast, proteasomes are dissociated into CP and RP and stored in membrane-less cytoplasmic foci, named proteasome storage granules (PSGs). With the resumption of growth, PSGs clear and mature proteasomes are transported into the nucleus by Blm10, a conserved 240 kDa protein and proteasome-intrinsic import receptor. How proteasomes are exported from the nucleus into the cytoplasm is unknown. KW - proteasome KW - nuclear transport KW - importin KW - karyopherin KW - Blm10 KW - proteasome storage granules Y1 - 2019 U6 - https://doi.org/10.3389/fmolb.2019.00034 SN - 2296-889X VL - 6 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Rosenbaum, Benjamin A1 - Raatz, Michael A1 - Weithoff, Guntram A1 - Fussmann, Gregor F. A1 - Gaedke, Ursula T1 - Estimating parameters from multiple time series of population dynamics using bayesian inference JF - Frontiers in ecology and evolution N2 - Empirical time series of interacting entities, e.g., species abundances, are highly useful to study ecological mechanisms. Mathematical models are valuable tools to further elucidate those mechanisms and underlying processes. However, obtaining an agreement between model predictions and experimental observations remains a demanding task. As models always abstract from reality one parameter often summarizes several properties. Parameter measurements are performed in additional experiments independent of the ones delivering the time series. Transferring these parameter values to different settings may result in incorrect parametrizations. On top of that, the properties of organisms and thus the respective parameter values may vary considerably. These issues limit the use of a priori model parametrizations. In this study, we present a method suited for a direct estimation of model parameters and their variability from experimental time series data. We combine numerical simulations of a continuous-time dynamical population model with Bayesian inference, using a hierarchical framework that allows for variability of individual parameters. The method is applied to a comprehensive set of time series from a laboratory predator-prey system that features both steady states and cyclic population dynamics. Our model predictions are able to reproduce both steady states and cyclic dynamics of the data. Additionally to the direct estimates of the parameter values, the Bayesian approach also provides their uncertainties. We found that fitting cyclic population dynamics, which contain more information on the process rates than steady states, yields more precise parameter estimates. We detected significant variability among parameters of different time series and identified the variation in the maximum growth rate of the prey as a source for the transition from steady states to cyclic dynamics. By lending more flexibility to the model, our approach facilitates parametrizations and shows more easily which patterns in time series can be explained also by simple models. Applying Bayesian inference and dynamical population models in conjunction may help to quantify the profound variability in organismal properties in nature. KW - Bayesian inference KW - chemostat experiments KW - ordinary differential equation KW - parameter estimation KW - population dynamics KW - predator prey KW - time series analysis KW - trait variability Y1 - 2019 U6 - https://doi.org/10.3389/fevo.2018.00234 SN - 2296-701X VL - 6 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Perkins, Daniel M. A1 - Perna, Andrea A1 - Adrian, Rita A1 - Cermeno, Pedro A1 - Gaedke, Ursula A1 - Huete-Ortega, Maria A1 - White, Ethan P. A1 - Yvon-Durocher, Gabriel T1 - Energetic equivalence underpins the size structure of tree and phytoplankton communities JF - Nature Communications N2 - The size structure of autotroph communities - the relative abundance of small vs. large individuals - shapes the functioning of ecosystems. Whether common mechanisms underpin the size structure of unicellular and multicellular autotrophs is, however, unknown. Using a global data compilation, we show that individual body masses in tree and phytoplankton communities follow power-law distributions and that the average exponents of these individual size distributions (ISD) differ. Phytoplankton communities are characterized by an average ISD exponent consistent with three-quarter-power scaling of metabolism with body mass and equivalence in energy use among mass classes. Tree communities deviate from this pattern in a manner consistent with equivalence in energy use among diameter size classes. Our findings suggest that whilst universal metabolic constraints ultimately underlie the emergent size structure of autotroph communities, divergent aspects of body size (volumetric vs. linear dimensions) shape the ecological outcome of metabolic scaling in forest vs. pelagic ecosystems. Y1 - 2019 U6 - https://doi.org/10.1038/s41467-018-08039-3 SN - 2041-1723 VL - 10 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Wickert, Andrew D. A1 - Schildgen, Taylor F. T1 - Long-profile evolution of transport-limited gravel-bed rivers JF - Earth surface dynamics N2 - Alluvial and transport-limited bedrock rivers constitute the majority of fluvial systems on Earth. Their long profiles hold clues to their present state and past evolution. We currently possess first-principles-based governing equations for flow, sediment transport, and channel morphodynamics in these systems, which we lack for detachment-limited bedrock rivers. Here we formally couple these equations for transport-limited gravel-bed river long-profile evolution. The result is a new predictive relationship whose functional form and parameters are grounded in theory and defined through experimental data. From this, we produce a power-law analytical solution and a finite-difference numerical solution to long-profile evolution. Steady-state channel concavity and steepness are diagnostic of external drivers: concavity decreases with increasing uplift rate, and steepness increases with an increasing sediment-to-water supply ratio. Constraining free parameters explains common observations of river form: to match observed channel concavities, gravel-sized sediments must weather and fine - typically rapidly - and valleys typically should widen gradually. To match the empirical square-root width-discharge scaling in equilibrium-width gravel-bed rivers, downstream fining must occur. The ability to assign a cause to such observations is the direct result of a deductive approach to developing equations for landscape evolution. Y1 - 2019 U6 - https://doi.org/10.5194/esurf-7-17-2019 SN - 2196-6311 SN - 2196-632X VL - 7 IS - 1 SP - 17 EP - 43 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Lonsdorf, Tina B. A1 - Klingelhöfer-Jens, Maren A1 - Andreatta, Marta A1 - Beckers, Tom A1 - Chalkia, Anastasia A1 - Gerlicher, Anna A1 - Jentsch, Valerie L. A1 - Drexler, Shira Meir A1 - Mertens, Gaetan A1 - Richter, Jan A1 - Sjouwerman, Rachel A1 - Wendt, Julia A1 - Merz, Christian J. T1 - Navigating the garden of forking paths for data exclusions in fear conditioning research JF - eLife N2 - In this report, we illustrate the considerable impact of researcher degrees of freedom with respect to exclusion of participants in paradigms with a learning element. We illustrate this empirically through case examples from human fear conditioning research, in which the exclusion of ‘non-learners’ and ‘non-responders’ is common – despite a lack of consensus on how to define these groups. We illustrate the substantial heterogeneity in exclusion criteria identified in a systematic literature search and highlight the potential problems and pitfalls of different definitions through case examples based on re-analyses of existing data sets. On the basis of these studies, we propose a consensus on evidence-based rather than idiosyncratic criteria, including clear guidelines on reporting details. Taken together, we illustrate how flexibility in data collection and analysis can be avoided, which will benefit the robustness and replicability of research findings and can be expected to be applicable to other fields of research that involve a learning element. Y1 - 2019 U6 - https://doi.org/10.7554/eLife.52465 SN - 2050-084X VL - 8 PB - eLife Sciences Publications CY - Cambridge ER - TY - JOUR A1 - Lonsdorf, Tina B. A1 - Klingelhöfer-Jens, Maren A1 - Andreatta, Marta A1 - Beckers, Tom A1 - Chalkia, Anastasia A1 - Gerlicher, Anna Maria Veronika A1 - Jentsch, Valerie L. A1 - Drexler, Shira Meir A1 - Mertens, Gaetan A1 - Richter, Jan A1 - Sjouwerman, Rachel A1 - Wendt, Julia A1 - Merz, Christian J. T1 - Navigating the garden of forking paths for data exclusions in fear conditioning research JF - eLife Y1 - 2019 U6 - https://doi.org/10.7554/eLife.52465 SN - 2050-084X VL - 8 PB - eLife Sciences Publications CY - Cambridge ER - TY - JOUR A1 - Günther, Erika A1 - Klauß, André A1 - Toro-Nahuelpan, Mauricio A1 - Schüler, Dirk A1 - Hille, Carsten A1 - Faivre, Damien T1 - The in vivo mechanics of the magnetotactic backbone as revealed by correlative FLIM-FRET and STED microscopy JF - Scientific reports N2 - Protein interaction and protein imaging strongly benefit from the advancements in time-resolved and superresolution fluorescence microscopic techniques. However, the techniques were typically applied separately and ex vivo because of technical challenges and the absence of suitable fluorescent protein pairs. Here, we show correlative in vivo fluorescence lifetime imaging microscopy Forster resonance energy transfer (FLIM-FRET) and stimulated emission depletion (STED) microscopy to unravel protein mechanics and structure in living cells. We use magnetotactic bacteria as a model system where two proteins, MamJ and MamK, are used to assemble magnetic particles called magnetosomes. The filament polymerizes out of MamK and the magnetosomes are connected via the linker MamJ. Our system reveals that bacterial filamentous structures are more fragile than the connection of biomineralized particles to this filament. More importantly, we anticipate the technique to find wide applicability for the study and quantification of biological processes in living cells and at high resolution. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-55804-5 SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Thomas, Jessica E. A1 - Carvalho, Gary R. A1 - Haile, James A1 - Rawlence, Nicolas J. A1 - Martin, Michael D. A1 - Ho, Simon Y. W. A1 - Sigfusson, Arnor P. A1 - Josefsson, Vigfus A. A1 - Frederiksen, Morten A1 - Linnebjerg, Jannie F. A1 - Castruita, Jose A. Samaniego A1 - Niemann, Jonas A1 - Sinding, Mikkel-Holger S. A1 - Sandoval-Velasco, Marcela A1 - Soares, Andre E. R. A1 - Lacy, Robert A1 - Barilaro, Christina A1 - Best, Juila A1 - Brandis, Dirk A1 - Cavallo, Chiara A1 - Elorza, Mikelo A1 - Garrett, Kimball L. A1 - Groot, Maaike A1 - Johansson, Friederike A1 - Lifjeld, Jan T. A1 - Nilson, Goran A1 - Serjeanston, Dale A1 - Sweet, Paul A1 - Fuller, Errol A1 - Hufthammer, Anne Karin A1 - Meldgaard, Morten A1 - Fjeldsa, Jon A1 - Shapiro, Beth A1 - Hofreiter, Michael A1 - Stewart, John R. A1 - Gilbert, M. Thomas P. A1 - Knapp, Michael T1 - Demographic reconstruction from ancient DNA supports rapid extinction of the great auk JF - eLife N2 - The great auk was once abundant and distributed across the North Atlantic. It is now extinct, having been heavily exploited for its eggs, meat, and feathers. We investigated the impact of human hunting on its demise by integrating genetic data, GPS-based ocean current data, and analyses of population viability. We sequenced complete mitochondrial genomes of 41 individuals from across the species' geographic range and reconstructed population structure and population dynamics throughout the Holocene. Taken together, our data do not provide any evidence that great auks were at risk of extinction prior to the onset of intensive human hunting in the early 16th century. In addition, our population viability analyses reveal that even if the great auk had not been under threat by environmental change, human hunting alone could have been sufficient to cause its extinction. Our results emphasise the vulnerability of even abundant and widespread species to intense and localised exploitation. Y1 - 2019 U6 - https://doi.org/10.7554/eLife.47509 SN - 2050-084X VL - 8 PB - eLife Sciences Publications CY - Cambridge ER - TY - JOUR A1 - Bröker, Katharine A1 - Sinelnikov, Evgeny A1 - Gustavus, Dirk A1 - Schumacher, Udo A1 - Pörtner, Ralf A1 - Hoffmeister, Hans A1 - Lüth, Stefan A1 - Dammermann, Werner T1 - Mass Production of Highly Active NK Cells for Cancer Immunotherapy in a GMP Conform Perfusion Bioreactor JF - Frontiers in Bioengineering and Biotechnology N2 - NK cells have emerged as promising candidates for cancer immunotherapy, especially due to their ability to fight circulating tumor cells thereby preventing metastases formation. Hence several studies have been performed to generate and expand highly cytotoxic NK cells ex vivo, e.g., by using specific cytokines to upregulate both their proliferation and surface expression of distinct activating receptors. Apart from an enhanced activity, application of NK cells as immunotherapeutic agent further requires sufficient cell numbers and a high purity. All these parameters depend on a variety of different factors including the starting material, additives like cytokines as well as the culture system. Here we analyzed PBMC-derived NK cells of five anonymized healthy donors expanded under specific conditions in an innovative perfusion bioreactor system with respect to their phenotype, IFN gamma production, and cytotoxicity in vitro. Important features of the meander type bioreactors used here are a directed laminar flow of medium and control of relevant process parameters. Cells are cultivated under "steady state" conditions in perfusion mode. Our data demonstrate that expansion of CD3(+) T cell depleted PBMCs in our standardized system generates massive amounts of highly pure (>85%) and potent anticancer active NK cells. These cells express a variety of important receptors driving NK cell recruitment, adhesion as well as activation. More specifically, they express the chemokine receptors CXCR3, CXCR4, and CCR7, the adhesion molecules L-selectin, LFA-1, and VLA-4, the activating receptors NKp30, NKp44, NKp46, NKG2D, DNAM1, and CD16 as well as the death ligands TRAIL and Fas-L. Moreover, the generated NK cells show a strong IFN gamma expression upon cultivation with K562 tumor cells and demonstrate a high cytotoxicity toward leukemic as well as solid tumor cell lines in vitro. Altogether, these characteristics promise a high clinical potency of thus produced NK cells awaiting further evaluation. KW - natural killer cells (NK cells) KW - cytotoxicity KW - tumor immunity KW - immunotherapy KW - perfusion bioreactor KW - GMP KW - mass production process Y1 - 2019 U6 - https://doi.org/10.3389/fbioe.2019.00194 SN - 2296-4185 VL - 7 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Löpfe, Moira A1 - Duss, Anja A1 - Zafeiropoulou, Katerina-Alexandra A1 - Bjoergvinsdottir, Oddny A1 - Eglin, David A1 - Fortunato, Giuseppino A1 - Klasen, Jürgen A1 - Ferguson, Stephen J. A1 - Würtz-Kozak, Karin A1 - Krupkova, Olga T1 - Electrospray-Based Microencapsulation of Epigallocatechin 3-Gallate for Local Delivery into the Intervertebral Disc JF - Pharmaceutics N2 - Locally delivered anti-inflammatory compounds can restore the homeostasis of the degenerated intervertebral disc (IVD). With beneficial effects on IVD cells, epigallocatechin 3-gallate (EGCG) is a promising therapeutic candidate. However, EGCG is prone to rapid degradation and/or depletion. Therefore, the purpose of this study was to develop a method for controlled EGCG delivery in the degenerated IVD. Primary IVD cells were isolated from human donors undergoing IVD surgeries. EGCG was encapsulated into microparticles by electrospraying of glutaraldehyde-crosslinked gelatin. The resulting particles were characterized in terms of cytocompatibility and anti-inflammatory activity, and combined with a thermoresponsive carrier to produce an injectable EGCG delivery system. Subsequently, electrospraying was scaled up using the industrial NANOSPIDER (TM) technology. The produced EGCG microparticles reduced the expression of inflammatory (IL-6, IL-8, COX-2) and catabolic (MMP1, MMP3, MMP13) mediators in pro-inflammatory 3D cell cultures. Combining the EGCG microparticles with the carrier showed a trend towards modulating EGCG activity/release. Electrospray upscaling was achieved, leading to particles with homogenous spherical morphologies. In conclusion, electrospray-based encapsulation of EGCG resulted in cytocompatible microparticles that preserved the activity of EGCG and showed the potential to control EGCG release, thus favoring IVD health by downregulating local inflammation. Future studies will focus on further exploring the biological activity of the developed delivery system for potential clinical use. KW - degenerative disc disease KW - inflammation KW - drug delivery KW - EGCG KW - microparticles KW - injectable biomaterial KW - electrospraying Y1 - 2019 U6 - https://doi.org/10.3390/pharmaceutics11090435 SN - 1999-4923 VL - 11 IS - 9 PB - MDPI CY - Basel ER - TY - JOUR A1 - Miele, Vincent A1 - Guill, Christian A1 - Ramos-Jiliberto, Rodrigo A1 - Kéfi, Sonia T1 - Non-trophic interactions strengthen the diversity-functioning relationship in an ecological bioenergetic network model JF - PLoS Computational Biology : a new community journal N2 - Ecological communities are undeniably diverse, both in terms of the species that compose them as well as the type of interactions that link species to each other. Despite this long recognition of the coexistence of multiple interaction types in nature, little is known about the consequences of this diversity for community functioning. In the ongoing context of global change and increasing species extinction rates, it seems crucial to improve our understanding of the drivers of the relationship between species diversity and ecosystem functioning. Here, using a multispecies dynamical model of ecological communities including various interaction types (e.g. competition for space, predator interference, recruitment facilitation in addition to feeding), we studied the role of the presence and the intensity of these interactions for species diversity, community functioning (biomass and production) and the relationship between diversity and functioning. Taken jointly, the diverse interactions have significant effects on species diversity, whose amplitude and sign depend on the type of interactions involved and their relative abundance. They however consistently increase the slope of the relationship between diversity and functioning, suggesting that species losses might have stronger effects on community functioning than expected when ignoring the diversity of interaction types and focusing on feeding interactions only. Y1 - 2020 U6 - https://doi.org/10.1371/journal.pcbi.1007269 SN - 1553-7358 VL - 15 IS - 8 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Rieck, Christoph Paul Kurt A1 - Geiger, Daniel A1 - Munkert, Jennifer A1 - Messerschmidt, Katrin A1 - Petersen, Jan A1 - Strasser, Juliane A1 - Meitinger, Nadine A1 - Kreis, Wolfgang T1 - Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae JF - Microbiologyopen N2 - A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5‐isomerase gene from Comamonas testosteronii, (c) a mutated steroid‐5β‐reductase gene from Arabidopsis thaliana, and (d) a steroid 21‐hydroxylase gene from Mus musculus. A second plasmid bearing an ADR/ADX fusion gene from Bos taurus was also constructed. A Saccharomyces cerevisiae strain bearing these two plasmids was generated. This strain, termed “CARD II yeast”, was capable of producing 5β‐pregnane‐3β,21‐diol‐20‐one, a central intermediate in 5β‐cardenolide biosynthesis, starting from pregnenolone which was added to the culture medium. Using this approach, five consecutive steps in cardenolide biosynthesis were realized in baker's yeast. Y1 - 2019 U6 - https://doi.org/10.1002/mbo3.925 SN - 2045-8827 VL - 8 IS - 12 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Shcherbakov, Robert A1 - Zhuang, Jiancang A1 - Zöller, Gert A1 - Ogata, Yosihiko T1 - Forecasting the magnitude of the largest expected earthquake JF - Nature Communications N2 - The majority of earthquakes occur unexpectedly and can trigger subsequent sequences of events that can culminate in more powerful earthquakes. This self-exciting nature of seismicity generates complex clustering of earthquakes in space and time. Therefore, the problem of constraining the magnitude of the largest expected earthquake during a future time interval is of critical importance in mitigating earthquake hazard. We address this problem by developing a methodology to compute the probabilities for such extreme earthquakes to be above certain magnitudes. We combine the Bayesian methods with the extreme value theory and assume that the occurrence of earthquakes can be described by the Epidemic Type Aftershock Sequence process. We analyze in detail the application of this methodology to the 2016 Kumamoto, Japan, earthquake sequence. We are able to estimate retrospectively the probabilities of having large subsequent earthquakes during several stages of the evolution of this sequence. Y1 - 2019 U6 - https://doi.org/10.1038/s41467-019-11958-4 SN - 2041-1723 VL - 10 PB - Nature Publishing Group CY - London ER - TY - JOUR A1 - Schiro, Gabriele A1 - Colangeli, Pierluigi A1 - Müller, Marina E. H. T1 - A Metabarcoding Analysis of the Mycobiome of Wheat Ears Across a Topographically Heterogeneous Field JF - Frontiers in microbiology KW - Fusarium KW - microclimate KW - canopy KW - fungal community KW - Alternaria KW - spatially induced variance Y1 - 2019 U6 - https://doi.org/10.3389/fmicb.2019.02095 SN - 1664-302X VL - 10 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Bielcik, Milos A1 - Aguilar-Trigueros, Carlos A. A1 - Lakovic, Milica A1 - Jeltsch, Florian A1 - Rillig, Matthias C. T1 - The role of active movement in fungal ecology and community assembly JF - Movement Ecology N2 - Movement ecology aims to provide common terminology and an integrative framework of movement research across all groups of organisms. Yet such work has focused on unitary organisms so far, and thus the important group of filamentous fungi has not been considered in this context. With the exception of spore dispersal, movement in filamentous fungi has not been integrated into the movement ecology field. At the same time, the field of fungal ecology has been advancing research on topics like informed growth, mycelial translocations, or fungal highways using its own terminology and frameworks, overlooking the theoretical developments within movement ecology. We provide a conceptual and terminological framework for interdisciplinary collaboration between these two disciplines, and show how both can benefit from closer links: We show how placing the knowledge from fungal biology and ecology into the framework of movement ecology can inspire both theoretical and empirical developments, eventually leading towards a better understanding of fungal ecology and community assembly. Conversely, by a greater focus on movement specificities of filamentous fungi, movement ecology stands to benefit from the challenge to evolve its concepts and terminology towards even greater universality. We show how our concept can be applied for other modular organisms (such as clonal plants and slime molds), and how this can lead towards comparative studies with the relationship between organismal movement and ecosystems in the focus. KW - Filamentous fungi KW - Microbial community KW - Active movement KW - Modular organisms KW - Interference competition KW - Fungal space searching algorithms KW - Fungal foraging KW - Fungal highways KW - Clonal plants KW - Slime molds Y1 - 2019 U6 - https://doi.org/10.1186/s40462-019-0180-6 SN - 2051-3933 VL - 7 IS - 1 PB - BMC CY - London ER - TY - JOUR A1 - Rosso, Enrico T1 - Ein „Zusammengehen ohne Zusammenkommen“. Der Entstehungsprozess des intellektuellen Netzwerkes um die Zeitschrift Die Kreatur JF - Naharaim N2 - Eine Gruppe, gar eine innerlich verbundene Gemeinschaft, können Intellektuelle im Normalfall nur bilden, soweit sie von gleichem Denken und Wollen bewegt werden […]. Wo dagegen die von den Intellektuellen vertretenen Ansichten weit divergieren, da kann es nur unter sehr kritischen Umständen geschehen, daß die verschieden gearteten (sozialen, politischen, rechtlichen, kulturellen, religiösen) Standpunkte und Anliegen – auf Zeit – sich zusammenfinden […]. Das polemische Element, das kritischen Intellektuellen ja wesensmäßig zu eigen ist, bringt am ehesten eine gemeinsame Front zwischen ihnen zustande. N2 - This research article aims to deliver an analytical representation of the intellectual environment of the culture journal Die Kreatur (1926-1930), a religious-dialogical quarterly edited by Martin Buber, Joseph Wittig, and Viktor von Weizsacker and enlivened by some of the most prominent figures of German-Jewish interwar culture (among others W. Benjamin, H. S. Bergman, E. Rosenstock-Huessy, F. Rosenzweig, E. Simon, and L. Strauss). Building on a recognition of the problematic relationship between the dialogical model claimed in the programmatic foreword of the journal and the group narratives enacted by its key contributors, the study attempts to outline the formation process of the intellectual plexus of the journal and to provide a differentiated analysis of its singular constituents via a sociologically informed framework. The representation of the intellectual network of Die Kreatur in accordance with the model of a "circle of circles" provides insight into the elusive dialectic of interaction and divergence that determines the relations between the main actors of the journal and thus contributes to unfold the interplay of biographical intersections and conceptual synergies, as well as incongruities, frictions, and contradictory instances that determines its editorial and philosophical profile. T2 - A "Going Together without Coming Together". The Process of Creating the intellectual Network around the magazine Die Kreatur KW - German-Jewish Literature KW - Cultural Journal KW - Buber KW - Martin KW - Rosenzweig KW - Franz KW - Die Kreatur Y1 - 2019 U6 - https://doi.org/10.1515/naha-2019-0006 SN - 1862-9148 SN - 1862-9156 VL - 13 IS - 1-2 SP - 73 EP - 97 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Schwarz, Anja T1 - Melancholia JF - Cultural studies review Y1 - 2019 U6 - https://doi.org/10.5130/csr.v25i2.6918 SN - 1837-8692 VL - 25 IS - 2 SP - 259 EP - 261 PB - Melbourne Univ. Press CY - Sydney ER - TY - JOUR A1 - Ziesemer, Florence A1 - Hüttel, Alexandra A1 - Balderjahn, Ingo T1 - Anti-Consumption JF - Sustainability N2 - Transcending the conventional debate around efficiency in sustainable consumption, anti-consumption patterns leading to decreased levels of material consumption have been gaining importance. Change agents are crucial for the promotion of such patterns, so there may be lessons for governance interventions that can be learnt from the every-day experiences of those who actively implement and promote sustainability in the field of anti-consumption. Eighteen social innovation pioneers, who engage in and diffuse practices of voluntary simplicity and collaborative consumption as sustainable options of anti-consumption share their knowledge and personal insights in expert interviews for this research. Our qualitative content analysis reveals drivers, barriers, and governance strategies to strengthen anti-consumption patterns, which are negotiated between the market, the state, and civil society. Recommendations derived from the interviews concern entrepreneurship, municipal infrastructures in support of local grassroots projects, regulative policy measures, more positive communication to strengthen the visibility of initiatives and emphasize individual benefits, establishing a sense of community, anti-consumer activism, and education. We argue for complementary action between top-down strategies, bottom-up initiatives, corporate activities, and consumer behavior. The results are valuable to researchers, activists, marketers, and policymakers who seek to enhance their understanding of materially reduced consumption patterns based on the real-life experiences of active pioneers in the field. KW - social innovation KW - sufficiency KW - collaborative consumption KW - expert interview KW - consumer behavior KW - sustainability KW - innovation policy KW - governance for sustainable development KW - consumer education Y1 - 2019 U6 - https://doi.org/10.3390/su11236663 SN - 2071-1050 VL - 11 IS - 23 PB - MDPI CY - Basel ER - TY - JOUR A1 - Ozcelikay, Goksu A1 - Kurbanoglu, Sevinc A1 - Zhang, Xiaorong A1 - Söz, Çağla Kosak A1 - Wollenberger, Ulla A1 - Ozkan, Sibel A. A1 - Yarman, Aysu A1 - Scheller, Frieder W. T1 - Electrochemical MIP Sensor for Butyrylcholinesterase JF - Polymers N2 - Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range. KW - molecularly imprinted polymers KW - biomimetic sensors KW - butyrylcholinesterase KW - o-phenylenediamine KW - rivastigmine Y1 - 2019 U6 - https://doi.org/10.3390/polym11121970 SN - 2073-4360 VL - 11 IS - 12 PB - MDPI CY - Basel ER - TY - JOUR A1 - Niven, Robert K. A1 - Abel, Markus A1 - Schlegel, Michael A1 - Waldrip, Steven H. T1 - Maximum Entropy Analysis of Flow Networks: Theoretical Foundation and Applications JF - Entropy N2 - The concept of a "flow network"-a set of nodes and links which carries one or more flows-unites many different disciplines, including pipe flow, fluid flow, electrical, chemical reaction, ecological, epidemiological, neurological, communications, transportation, financial, economic and human social networks. This Feature Paper presents a generalized maximum entropy framework to infer the state of a flow network, including its flow rates and other properties, in probabilistic form. In this method, the network uncertainty is represented by a joint probability function over its unknowns, subject to all that is known. This gives a relative entropy function which is maximized, subject to the constraints, to determine the most probable or most representative state of the network. The constraints can include "observable" constraints on various parameters, "physical" constraints such as conservation laws and frictional properties, and "graphical" constraints arising from uncertainty in the network structure itself. Since the method is probabilistic, it enables the prediction of network properties when there is insufficient information to obtain a deterministic solution. The derived framework can incorporate nonlinear constraints or nonlinear interdependencies between variables, at the cost of requiring numerical solution. The theoretical foundations of the method are first presented, followed by its application to a variety of flow networks. KW - maximum entropy analysis KW - flow network KW - probabilistic inference Y1 - 2019 U6 - https://doi.org/10.3390/e21080776 SN - 1099-4300 VL - 21 IS - 8 SP - 776 PB - MDPI CY - Basel ER - TY - JOUR A1 - Posovszky, Carsten A1 - Roesler, Vreni Helen A1 - Becker, Sebastian A1 - Iven, Enno A1 - Hudert, Christian A1 - Ebinger, Friedrich A1 - Calvano, Claudia A1 - Warschburger, Petra T1 - Roles of Lactose and Fructose Malabsorption and Dietary Outcomes in Children Presenting with Chronic Abdominal Pain JF - Nutrients N2 - Intolerance to lactose or fructose is frequently diagnosed in children with chronic abdominal pain (CAP). However, the causal relationship remains a matter of discussion. A cohort of 253 patients, aged 7-12 years, presenting with unexplained CAP received standardized diagnostics. Additional diagnostic tests were performed based on their medical history and physical and laboratory investigations. Fructose and lactose hydrogen breath tests (H2BT) as well as empiric diagnostic elimination diets were performed in 135 patients reporting abdominal pain related to the consumption of lactose or fructose to evaluate carbohydrate intolerance as a potential cause of CAP. Carbohydrate malabsorption by H2BT was found in 55 (41%) out of 135 patients. An abnormal increase in H2BT was revealed in 30% (35/118) of patients after fructose consumption and in 18% (20/114) of patients after lactose administration. Forty-six percent (25/54) reported pain relief during a diagnostic elimination diet. In total, 17 patients had lactose malabsorption, 29 fructose malabsorption, and nine combined carbohydrate malabsorption. Carbohydrate intolerance as a cause of CAP was diagnosed at follow-up in only 18% (10/55) of patients with malabsorption after the elimination of the respective carbohydrate. Thus, carbohydrate malabsorption appears to be an incidental finding in children with functional abdominal pain disorders, rather than its cause. Therefore, testing of carbohydrate intolerance should only be considered in children with a strong clinical suspicion and with the goal to prevent long-term unnecessary dietary restrictions in children suffering from CAP. KW - chronic abdominal pain KW - children KW - fructose malabsorption KW - lactose intolerance KW - hydrogen breath test KW - functional abdominal pain disorders Y1 - 2019 U6 - https://doi.org/10.3390/nu11123063 SN - 2072-6643 VL - 11 IS - 12 PB - MDPI CY - Basel ER - TY - JOUR A1 - Codutti, Agnese A1 - Bente, Klaas A1 - Faivre, Damien A1 - Klumpp, Stefan T1 - Chemotaxis in external fields: Simulations for active magnetic biological matter JF - PLoS Computational Biology : a new community journal N2 - The movement of microswimmers is often described by active Brownian particle models. Here we introduce a variant of these models with several internal states of the swimmer to describe stochastic strategies for directional swimming such as run and tumble or run and reverse that are used by microorganisms for chemotaxis. The model includes a mechanism to generate a directional bias for chemotaxis and interactions with external fields (e.g., gravity, magnetic field, fluid flow) that impose forces or torques on the swimmer. We show how this modified model can be applied to various scenarios: First, the run and tumble motion of E. coli is used to establish a paradigm for chemotaxis and investigate how it is affected by external forces. Then, we study magneto-aerotaxis in magnetotactic bacteria, which is biased not only by an oxygen gradient towards a preferred concentration, but also by magnetic fields, which exert a torque on an intracellular chain of magnets. We study the competition of magnetic alignment with active reorientation and show that the magnetic orientation can improve chemotaxis and thereby provide an advantage to the bacteria, even at rather large inclination angles of the magnetic field relative to the oxygen gradient, a case reminiscent of what is expected for the bacteria at or close to the equator. The highest gain in chemotactic velocity is obtained for run and tumble with a magnetic field parallel to the gradient, but in general a mechanism for reverse motion is necessary to swim against the magnetic field and a run and reverse strategy is more advantageous in the presence of a magnetic torque. This finding is consistent with observations that the dominant mode of directional changes in magnetotactic bacteria is reversal rather than tumbles. Moreover, it provides guidance for the design of future magnetic biohybrid swimmers. Author summary In this paper, we propose a modified Active Brownian particle model to describe bacterial swimming behavior under the influence of external forces and torques, in particular of a magnetic torque. This type of interaction is particularly important for magnetic biohybrids (i.e. motile bacteria coupled to a synthetic magnetic component) and for magnetotactic bacteria (i.e. bacteria with a natural intracellular magnetic chain), which perform chemotaxis to swim along chemical gradients, but are also directed by an external magnetic field. The model allows us to investigate the benefits and disadvantages of such coupling between two different directionality mechanisms. In particular we show that the magnetic torque can speed chemotaxis up in some conditions, while it can hinder it in other cases. In addition to an understanding of the swimming strategies of naturally magnetotactic organisms, the results may guide the design of future biomedical devices. Y1 - 2019 U6 - https://doi.org/10.1371/journal.pcbi.1007548 SN - 1553-734X SN - 1553-7358 VL - 15 IS - 12 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Mei, Shilin A1 - Kochovski, Zdravko A1 - Roa, Rafael A1 - Gu, Sasa A1 - Xu, Xiaohui A1 - Yu, Hongtao A1 - Dzubiella, Joachim A1 - Ballauff, Matthias A1 - Lu, Yan T1 - Enhanced Catalytic Activity of Gold@Polydopamine Nanoreactors with Multi-compartment Structure Under NIR Irradiation JF - Nano-Micro Letters N2 - Photothermal conversion (PTC) nanostructures have great potential for applications in many fields, and therefore, they have attracted tremendous attention. However, the construction of a PTC nanoreactor with multi-compartment structure to achieve the combination of unique chemical properties and structural feature is still challenging due to the synthetic difficulties. Herein, we designed and synthesized a catalytically active, PTC gold (Au)@polydopamine (PDA) nanoreactor driven by infrared irradiation using assembled PS-b-P2VP nanosphere as soft template. The particles exhibit multi-compartment structure which is revealed by 3D electron tomography characterization technique. They feature permeable shells with tunable shell thickness. Full kinetics for the reduction reaction of 4-nitrophenol has been investigated using these particles as nanoreactors and compared with other reported systems. Notably, a remarkable acceleration of the catalytic reaction upon near-infrared irradiation is demonstrated, which reveals for the first time the importance of the synergistic effect of photothermal conversion and complex inner structure to the kinetics of the catalytic reduction. The ease of synthesis and fresh insights into catalysis will promote a new platform for novel nanoreactor studies. KW - Gold@polydopamine KW - 3D tomography KW - Nanoreactor KW - Catalysis KW - Photothermal conversion Y1 - 2019 U6 - https://doi.org/10.1007/s40820-019-0314-9 SN - 2311-6706 SN - 2150-5551 VL - 11 IS - 1 PB - Shanghai JIAO TONG univ press CY - Shanghai ER - TY - JOUR A1 - Saha, Sourav A1 - Owen, Lewis A. A1 - Orr, Elizabeth N. A1 - Caffee, Marc W. T1 - Cosmogenic Be-10 and equilibrium-line altitude dataset of Holocene glacier advances in the Himalayan-Tibetan orogen JF - Data in brief N2 - A comprehensive analysis of the variable temporal and spatial responses of tropical-subtropical high-altitude glaciers to climate change is critical for successful model predictions and environmental risk assessment in the Himalayan-Tibetan orogen. High-frequency Holocene glacier chronostratigraphies are therefore reconstructed in 79 glaciated valleys across the orogen using 519 published and 16 new terrestrial cosmogenic 10Be exposure age dataset. Published 10Be ages are compiled only for moraine boulders (excluding bedrock ages). These ages are recalculated using the latest ICE-D production rate calibration database and the scaling scheme models. Outliers for the individual moraine are detected using the Chauvenet's criterion. In addition, past equilibrium-line altitudes (ELAs) are determined using the area-altitude (AA), area accumulation ratio (AAR), and toe-headwall accumulation ratio (THAR) methods for each glacier advance. The modern maximum elevations of lateral moraines (MELM) are also used to estimate modern ELAs and as an independent check on mean ELAs derived using the above three methods. These data may serve as an essential archive for future studies focusing on the cryospheric and environmental changes in the Himalayan-Tibetan orogen. A more comprehensive analysis of the published and new 10Be ages and ELA results and a list of references are presented in Saha et al. (2019, High-frequency Holocene glacier fluctuations in the Himalayan-Tibetan orogen. Quaternary Science Reviews, 220, 372–400). KW - Cosmogenic nuclides KW - Equilibrium-line altitudes KW - Holocene KW - Central asia KW - Glaciation Y1 - 2019 U6 - https://doi.org/10.1016/j.dib.2019.104412 SN - 2352-3409 VL - 26 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Rossberg, Axel G. A1 - Gaedke, Ursula A1 - Kratina, Pavel T1 - Dome patterns in pelagic size spectra reveal strong trophic cascades JF - Nature Communications N2 - In ecological communities, especially the pelagic zones of aquatic ecosystems, certain bodysize ranges are often over-represented compared to others. Community size spectra, the distributions of community biomass over the logarithmic body-mass axis, tend to exhibit regularly spaced local maxima, called "domes", separated by steep troughs. Contrasting established theory, we explain these dome patterns as manifestations of top-down trophic cascades along aquatic food chains. Compiling high quality size-spectrum data and comparing these with a size-spectrum model introduced in this study, we test this theory and develop a detailed picture of the mechanisms by which bottom-up and top-down effects interact to generate dome patterns. Results imply that strong top-down trophic cascades are common in freshwater communities, much more than hitherto demonstrated, and may arise in nutrient rich marine systems as well. Transferring insights from the general theory of nonlinear pattern formation to domes patterns, we provide new interpretations of past lake-manipulation experiments. Y1 - 2019 U6 - https://doi.org/10.1038/s41467-019-12289-0 SN - 2041-1723 VL - 10 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Franz, Kristina A1 - Otten, Lindsey A1 - Müller-Werdan, Ursula A1 - Döhner, Wolfram A1 - Norman, Kristina T1 - Severe Weight Loss and Its Association with Fatigue in Old Patients at Discharge from a Geriatric Hospital JF - Nutrients N2 - Although malnutrition is frequent in the old, little is known about its association with fatigue. We evaluated the relation of self-reported severe weight loss with fatigue and the predictors for fatigue in old patients at hospital discharge. Severe weight loss was defined according to involuntary weight loss >= 5% in the last three months. We determined fatigue with the validated Brief Fatigue Inventory questionnaire. The regression analyses were adjusted for age, sex, number of comorbidities, medications/day, and BMI. Of 424 patients aged between 61 and 98 y, 34.1% had severe weight loss. Fatigue was higher in patients with severe weight loss (3.7 +/- 2.3 vs. 3.2 +/- 2.3 points, p = 0.021). In a multinomial regression model, weight loss was independently associated with higher risk for moderate fatigue (OR:1.172, CI:1.026-1.338, p = 0.019) and with increased risk for severe fatigue (OR:1.209, CI:1.047-1.395, p = 0.010) together with the number of medications/day (OR:1.220, CI:1.023-1.455, p = 0.027). In a binary regression model, severe weight loss predicted moderate-to-severe fatigue in the study population (OR:1.651, CI:1.052-2.590, p = 0.029). In summary, patients with self-reported severe weight loss at hospital discharge exhibited higher fatigue levels and severe weight loss was an independent predictor of moderate and severe fatigue, placing these patients at risk for impaired outcome in the post-hospital period. KW - malnutrition KW - involuntary weight loss KW - post-hospital syndrome KW - fatigue KW - old adults Y1 - 2019 U6 - https://doi.org/10.3390/nu11102415 SN - 2072-6643 VL - 11 IS - 10 PB - MDPI CY - Basel ER - TY - JOUR A1 - Palyulin, Vladimir V. A1 - Blackburn, George A1 - Lomholt, Michael A. A1 - Watkins, Nicholas W. A1 - Metzler, Ralf A1 - Klages, Rainer A1 - Chechkin, Aleksei V. T1 - First passage and first hitting times of Levy flights and Levy walks JF - New journal of physics : the open-access journal for physics N2 - For both Lévy flight and Lévy walk search processes we analyse the full distribution of first-passage and first-hitting (or first-arrival) times. These are, respectively, the times when the particle moves across a point at some given distance from its initial position for the first time, or when it lands at a given point for the first time. For Lévy motions with their propensity for long relocation events and thus the possibility to jump across a given point in space without actually hitting it ('leapovers'), these two definitions lead to significantly different results. We study the first-passage and first-hitting time distributions as functions of the Lévy stable index, highlighting the different behaviour for the cases when the first absolute moment of the jump length distribution is finite or infinite. In particular we examine the limits of short and long times. Our results will find their application in the mathematical modelling of random search processes as well as computer algorithms. KW - Levy flights KW - Levy walks KW - first-passage time KW - first-hitting time Y1 - 2019 U6 - https://doi.org/10.1088/1367-2630/ab41bb SN - 1367-2630 VL - 21 IS - 10 PB - IOP Publ. Ltd. CY - Bristol ER - TY - JOUR A1 - Matthey-Doret, Cyril A1 - van der Kooi, Casper J. A1 - Jeffries, Daniel L. A1 - Bast, Jens A1 - Dennis, Alice B. A1 - Vorburger, Christoph A1 - Schwander, Tanja T1 - Mapping of Multiple Complementary Sex Determination Loci in a Parasitoid Wasp JF - Genome biology and evolution N2 - Sex determination has evolved in a variety of ways and can depend on environmental and genetic signals. A widespread form of genetic sex determination is haplodiploidy, where unfertilized, haploid eggs develop into males and fertilized diploid eggs into females. One of the molecular mechanisms underlying haplodiploidy in Hymenoptera, the large insect order comprising ants, bees, and wasps, is complementary sex determination (CSD). In species with CSD, heterozygosity at one or several loci induces female development. Here, we identify the genomic regions putatively underlying multilocus CSD in the parasitoid wasp Lysiphlebus fabarum using restriction -site associated DNA sequencing. By analyzing segregation patterns at polymorphic sites among 331 diploid males and females, we identify up to four CSD candidate regions, all on different chromosomes. None of the candidate regions feature evidence for homology with the csd gene from the honey bee, the only species in which CSD has been characterized, suggesting that CSD in L. fabarum is regulated via a novel molecular mechanism. Moreover, no homology is shared between the candidate loci, in contrast to the idea that multilocus CSD should emerge from duplications of an ancestral single -locus system. Taken together, our results suggest that the molecular mechanisms underlying CSD in Hymenoptera are not conserved between species, raising the question as to whether CSD may have evolved multiple times independently in the group. KW - hymenoptera KW - sex determination KW - Lysiphlebus fabarum KW - CSD Y1 - 2019 U6 - https://doi.org/10.1093/gbe/evz219 SN - 1759-6653 VL - 11 IS - 10 SP - 2954 EP - 2962 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Caliendo, Marco A1 - Goethner, Maximilian A1 - Weißenberger, Martin T1 - Entrepreneurial persistence beyond survival: Measurement and determinants JF - Journal of Small Business Management N2 - Entrepreneurial persistence is demonstrated by an entrepreneur’s continued positive maintenance of entrepreneurial motivation and constantly renewed active engagement in a new business venture despite counterforces or enticing alternatives. It thus is a crucial factor for entrepreneurs when pursuing and exploiting their business opportunities and in realizing potential economic gains and benefits. Using rich data on a representative sample of German business founders, we investigated the determinants of entrepreneurial persistence. Next to observed survival, we also constructed a hybrid persistence measure capturing the motivational dimension of persistence. We analyzed the influence of individual-level (human capital and personality) and business-related characteristics on both measures as well as their relative importance. We found that the two indicators emphasize different aspects of persistence. For the survival indicator, the predictive power was concentrated in business characteristics and human capital, while for hybrid persistence the dominant factors were business characteristics and personality. Finally, we showed that results were heterogeneous across subgroups. In particular, formerly unemployed founders did not differ in survival chances, but they were more likely to lack a high psychological commitment to their business ventures. KW - entrepreneurship KW - startups KW - persistence KW - survival Y1 - 2019 VL - 58 IS - 3 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Balderas-Valadez, Ruth Fabiola A1 - Schürmann, Robin Mathis A1 - Pacholski, Claudia T1 - One Spot-Two Sensors: Porous Silicon Interferometers in Combination With Gold Nanostructures Showing Localized Surface Plasmon Resonance JF - Frontiers in chemistry N2 - Sensors composed of a porous silicon monolayer covered with a film of nanostructured gold layer, which provide two optical signal transduction methods, are fabricated and thoroughly characterized concerning their sensing performance. For this purpose, silicon substrates were electrochemically etched in order to obtain porous silicon monolayers, which were subsequently immersed in gold salt solution facilitating the formation of a porous gold nanoparticle layer on top of the porous silicon. The deposition process was monitored by reflectance spectroscopy, and the appearance of a dip in the interference pattern of the porous silicon layer was observed. This dip can be assigned to the absorption of light by the deposited gold nanostructures leading to localized surface plasmon resonance. The bulk sensitivity of these sensors was determined by recording reflectance spectra in media having different refractive indices and compared to sensors exclusively based on porous silicon or gold nanostructures. A thorough analysis of resulting shifts of the different optical signals in the reflectance spectra on the wavelength scale indicated that the optical response of the porous silicon sensor is not influenced by the presence of a gold nanostructure on top. Moreover, the adsorption of thiol-terminated polystyrene to the sensor surface was solely detected by changes in the position of the dip in the reflectance spectrum, which is assigned to localized surface plasmon resonance in the gold nanostructures. The interference pattern resulting from the porous silicon layer is not shifted to longer wavelengths by the adsorption indicating the independence of the optical response of the two nanostructures, namely porous silicon and nanostructured gold layer, to refractive index changes and pointing to the successful realization of two sensors in one spot. KW - porous silicon KW - interferometry KW - gold nanostructures KW - surface plasmon resonance KW - optical sensor Y1 - 2019 U6 - https://doi.org/10.3389/fchem.2019.00593 SN - 2296-2646 VL - 7 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Zoll, Felix A1 - Diehl, Katharina A1 - Siebert, Rosemarie T1 - Integrating sustainability goals in innovation processes BT - applying a decision support tool in a dual-purpose chicken case study JF - Sustainability N2 - The innovative dual-purpose chicken approach aims at contributing to the transition towards sustainable poultry production by avoiding the culling of male chickens. To successfully integrate sustainability aspects into innovation, goal congruency among actors and clearly communicating the added value within the actor network and to consumers is needed. The challenge of identifying common sustainability goals calls for decision support tools. The objectives of our research were to investigate whether the tool could assist in improving communication and marketing with respect to sustainability and optimizing the value chain organization. Three actor groups participated in the tool application, in which quantitative and qualitative data were collected. The results showed that there were manifold sustainability goals within the innovation network, but only some goals overlapped, and the perception of their implementation also diverged. While easily marketable goals such as ‘animal welfare’ were perceived as being largely implemented, economic goals were prioritized less often, and the implementation was perceived as being rather low. By visualizing congruencies and differences in the goals, the tool helped identify fields of action, such as improved information flows and prompted thinking processes. We conclude that the tool is useful for managing complex decision processes with several actors involved. KW - value-based sustainability assessment KW - stakeholder participation KW - niche level KW - culling of male chickens KW - mixed methods Y1 - 2019 U6 - https://doi.org/10.3390/su11143761 SN - 2071-1050 VL - 11 IS - 14 PB - MDPI CY - Basel ER - TY - JOUR A1 - Starzonek, Janine A1 - Roscher, Katja A1 - Blither, Matthias A1 - Blaue, Dominique A1 - Schedlbauer, Carola A1 - Hire, Manuela A1 - Raila, Jens A1 - Vervuert, Ingrid T1 - Effects of a blend of green tea and curcuma extract supplementation on lipopolysaccharide-induced inflammation in horses and ponies JF - PeerJ N2 - Background. In horses and ponies numerous medical conditions are known to be linked with inflammation in different tissues, especially in the liver. Besides affecting other metabolic pathways such as the expression of certain interleukins (IL), inflammation is associated with stress of the endoplasmic reticulum (ER). In particular, ER stress leads to adaptive stress response and can be measured by several markers of inflammatory and stress signalling pathways, like nuclear factor kappa B (NF-kB). Objectives. To investigate lipopolysaccharide (LPS)-induced inflammatory reactions and their modulation in horses and ponies by feeding a polyphenol-rich supplement consisting of green tea and curcuma. Methods. In a cross-over study, 11 animals were allocated to either a placebo or a supplement group and supplemented with 10 g of a blend of green tea and curcuma extract (GCE) or a placebo (calcium carbonate) once daily. After 21 days of supplementation, all animals underwent a LPS challenge to induce moderate systemic inflammation. Blood samples and liver biopsies were taken at standardized time points: 24 hours before and 12 hours after LPS challenge. Inflammatory blood parameters such as serum amyloid A (SAA), haptoglobin and retinol binding protein 4 (RBP4) were measured in serum. Hepatic mRNA levels of selected markers of inflammation such as haptoglobin, tumor necrosis factor alpha (TNF-alpha), IL-1 beta, IL-6, cluster of differentiation 68 (CD68), fibroblast growth factor 21 (FGF-21), NF-kappa B, activating transcription factor 4 (ATF4) were quantified by RT-qPCR. In addition, liver biopsies were examined histologically for inflammatory alterations. Results. Blood markers of acute inflammatory response increased after LPS challenge. In the liver, the proinflammatory cytokine IL-1 beta showed significantly lower mRNA levels after LPS challenge in the supplemented group (P = 0.04) compared to the placebo group. Levels of the hepatic CD68 mRNA increased significantly in the placebo group (P = 0.04). There were no significant differences between supplemented and placebo groups concerning other markers of inflammation and markers of ER stress within the liver. The number of hepatic macrophages were not different after LPS challenge in both feeding groups. Conclusion. LPS was able to induce inflammation but seemed less suitable to induce ER stress in the horses and ponies. The polyphenol-rich supplement showed some potential to reduce inflammatory responses. Nevertheless, the supplementation did not exert an overall anti-inflammatory effect in horses and ponies. KW - Curcumin KW - Catechin KW - Equines KW - ER-stress KW - Polyphenols Y1 - 2019 U6 - https://doi.org/10.7717/peerj.8053 SN - 2167-8359 VL - 7 PB - PeerJ Inc. CY - London ER - TY - JOUR A1 - Aloni, Sapir Shekef A1 - Perovic, Milena A1 - Weitman, Michal A1 - Cohen, Reut A1 - Oschatz, Martin A1 - Mastai, Yitzhak T1 - Amino acid-based ionic liquids as precursors for the synthesis of chiral nanoporous carbons JF - Nanoscale Advances N2 - The synthesis of chiral nanoporous carbons based on chiral ionic liquids (CILs) of amino acids as precursors is described. Such unique precursors for the carbonization of CILs yield chiral carbonaceous materials with high surface area (approximate to 620 m(2) g(-1)). The enantioselectivities of the porous carbons are examined by advanced techniques such as selective adsorption of enantiomers using cyclic voltammetry, isothermal titration calorimetry, and mass spectrometry. These techniques demonstrate the chiral nature and high enantioselectivity of the chiral carbon materials. Overall, we believe that the novel approach presented here can contribute significantly to the development of new chiral carbon materials that will find important applications in chiral chemistry, such as in chiral catalysis and separation and in chiral sensors. From a scientific point of view, the approach and results reported here can significantly deepen our understanding of chirality at the nanoscale and of the structure and nature of chiral nonporous materials and surfaces. Y1 - 2019 U6 - https://doi.org/10.1039/c9na00520j SN - 2516-0230 VL - 1 IS - 12 SP - 4981 EP - 4988 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Batsios, Petros A1 - Gräf, Ralph A1 - Koonce, Michael P. A1 - Larochelle, Denis A. A1 - Meyer, Irene T1 - Nuclear envelope organization in Dictyostelium discoideum JF - The international journal of developmental biology N2 - The nuclear envelope consists of the outer and the inner nuclear membrane, the nuclear lamina and the nuclear pore complexes, which regulate nuclear import and export.The major constituent of the nuclear lamina of Dictyostelium is the lamin NE81. It can form filaments like B-type lamins and it interacts with Sun 1, as well as with the LEM/HeH-family protein Src1. Sun 1 and Src1 are nuclear envelope transmembrane proteins involved in the centrosome-nucleus connection and nuclear envelope stability at the nucleolar regions, respectively. In conjunction with a KASH-domain protein, Sun 1 usually forms a so-called LINC complex.Two proteins with functions reminiscent of KASH-domain proteins at the outer nuclear membrane of Dictyostelium are known; interaptin which serves as an actin connector and the kinesin Kif9 which plays a role in the microtubule-centrosome connector. However, both of these lack the conserved KASH-domain. The link of the centrosome to the nuclear envelope is essential for the insertion of the centrosome into the nuclear envelope and the appropriate spindle formation. Moreover, centrosome insertion is involved in perm eabilization of the mitotic nucleus, which ensures access of tubulin dimers and spindle assembly factors. Our recent progress in identifying key molecular players at the nuclear envelope of Dictyostelium promises further insights into the mechanisms of nuclear envelope dynamics. KW - nuclear envelop KW - Dictyostelium KW - lamin KW - NET KW - centrosome KW - centromere Y1 - 2019 U6 - https://doi.org/10.1387/ijdb.190184rg SN - 0214-6282 SN - 1696-3547 VL - 63 IS - 8-10 SP - 509 EP - 519 PB - UBC Pr CY - Bilbao ER - TY - JOUR A1 - Bürger, Gerd T1 - A seamless filter for daily to seasonal forecasts, with applications to Iran and Brazil JF - Quarterly Journal of the Royal Meteorological Society N2 - A digital filter is introduced which treats the problem of predictability versus time averaging in a continuous, seamless manner. This seamless filter (SF) is characterized by a unique smoothing rule that determines the strength of smoothing in dependence on lead time. The rule needs to be specified beforehand, either by expert knowledge or by user demand. As a result, skill curves are obtained that allow a predictability assessment across a whole range of time-scales, from daily to seasonal, in a uniform manner. The SF is applied to downscaled SEAS5 ensemble forecasts for two focus regions in or near the tropical belt, the river basins of the Karun in Iran and the Sao Francisco in Brazil. Both are characterized by strong seasonality and semi-aridity, so that predictability across various time-scales is in high demand. Among other things, it is found that from the start of the water year (autumn), areal precipitation is predictable with good skill for the Karun basin two and a half months ahead; for the Sao Francisco it is only one month, longer-term prediction skill is just above the critical level. KW - climate drift KW - ensemble prediction KW - seamless prediction KW - seasonal forecast skill Y1 - 2019 VL - 146 IS - 726 PB - WILEY-VCH CY - Weinheim ER - TY - JOUR A1 - Latimer, Andrew M. A1 - Jacobs, Brooke S. A1 - Gianoli, Ernesto A1 - Heger, Tina A1 - Salgado-Luarte, Cristian T1 - Parallel functional differentiation of an invasive annual plant on two continents JF - AoB PLANTS N2 - Rapid local adaptation frequently occurs during the spread of invading species. It remains unclear, however, how consistent, and therefore potentially predictable, such patterns of local adaptation are. One approach to this question is to measure patterns of local differentiation in functional traits and plasticity levels in invasive species in multiple regions. Finding consistent patterns of local differentiation in replicate regions suggests that these patterns are adaptive. Further, this outcome indicates that the invading species likely responds predictably to selection along environmental gradients, even though standing genetic variation is likely to have been reduced during introduction. We studied local differentiation in the invasive annual plant Erodium cicutarium in two invaded regions, California and Chile. We collected seeds from across strong gradients in precipitation and temperature in Mediterranean-climate parts of the two regions (10 populations per region). We grew seeds from maternal families from these populations through two generations and exposed the second generation to contrasting levels of water and nutrient availability. We measured growth, flowering time and leaf functional traits across these treatments to obtain trait means and plasticity measures. We found strong differentiation among populations in all traits. Plants from drier environments flowered earlier, were less plastic in flowering time and reached greater size in all treatments. Correlations among traits within regions suggested a coordinated evolutionary response along environmental gradients associated with growing season length. There was little divergence in traits and trait intercorrelations between regions, but strongly parallel divergence in traits within regions. Similar, statistically consistent patterns of local trait differentiation across two regions suggest that local adaptation to environmental gradients has aided the spread of this invasive species, and that the formation of ecotypes in newly invaded environments has been relatively consistent and predictable. KW - Erodium cicutarium KW - flowering time KW - functional trait correlations KW - invasive species KW - life-history strategy KW - local adaptation KW - parallel evolution KW - phenotypic plasticity Y1 - 2019 U6 - https://doi.org/10.1093/aobpla/plz010 SN - 2041-2851 VL - 11 IS - 2 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Javhar, Aminov A1 - Chen, Xi A1 - Bao, Anming A1 - Jamshed, Aminov A1 - Yunus, Mamadjanov A1 - Jovid, Aminov A1 - Latipa, Tuerhanjiang T1 - Comparison of Multi-Resolution Optical Landsat-8, Sentinel-2 and Radar Sentinel-1 Data for Automatic Lineament Extraction BT - A Case Study of Alichur Area, SE Pamir JF - Remote sensing N2 - Lineament mapping, which is an important part of any structural geological investigation, is made more efficient and easier by the availability of optical as well as radar remote sensing data, such as Landsat and Sentinel with medium and high spatial resolutions. However, the results from these multi-resolution data vary due to their difference in spatial resolution and sensitivity to soil occupation. The accuracy and quality of extracted lineaments depend strongly on the spatial resolution of the imagery. Therefore, the aim of this study was to compare the optical Landsat-8, Sentinel-2A, and radar Sentinel-1A satellite data for automatic lineament extraction. The framework of automatic approach includes defining the optimal parameters for automatic lineament extraction with a combination of edge detection and line-linking algorithms and determining suitable bands from optical data suited for lineament mapping in the study area. For the result validation, the extracted lineaments are compared against the manually obtained lineaments through the application of directional filtering and edge enhancement as well as to the lineaments digitized from the existing geological maps of the study area. In addition, a digital elevation model (DEM) has been utilized for an accuracy assessment followed by the field verification. The obtained results show that the best correlation between automatically extracted lineaments, manual interpretation, and the preexisting lineament map is achieved from the radar Sentinel-1A images. The tests indicate that the radar data used in this study, with 5872 and 5865 lineaments extracted from VH and VV polarizations respectively, is more efficient for structural lineament mapping than the Landsat-8 and Sentinel-2A optical imagery, from which 2338 and 4745 lineaments were extracted respectively. KW - image enhancement KW - automatic lineament extraction KW - Landsat-8 KW - Sentinel-1 KW - Sentinel-2 KW - structural mapping Y1 - 2019 U6 - https://doi.org/10.3390/rs11070778 SN - 2072-4292 VL - 11 IS - 7 PB - MDPI CY - Basel ER - TY - JOUR A1 - Festman, Julia A1 - Schwieter, John W. T1 - Self-concepts in reading and spelling among mono- and multilingual Children BT - Extending the bilingual advantage JF - Behavioral Sciences N2 - Cognitive representations and beliefs are what comprise an individual’s self-concept. A positive self-concept is related to and influences academic achievement, and the relationship between a domain-specific self-concept and achievement in the same domain is positive and strong. However, insufficient attention has been paid to these issues among multilingual children. More importantly, since instruction strongly contributes to the development of metacognition and executive functions (EFs), and since the bilingual advantage hypothesis holds that the constant management of multiple languages entails benefits for EF, we bring together these important issues in the present study. We examine the relationship between domain-specific self-concepts and standardized assessment of reading and spelling competences against the background of potential differences in self-concept between monolingual and multilingual German children. While between-group comparisons revealed no significant differences for self-concept nor reading competency, monolinguals outperformed multilinguals in spelling. Correlations between domain-specific self-concepts and academic achievement in reading comprehension, reading fluency, and spelling were positive and significant for both groups. Regardless of language background, children’s evaluations of their academic achievement (reading and spelling) were realistic. We argue, on a theoretical basis, that metacognition and EFs could facilitate a bilingual advantage and improve educational outcomes. KW - domain-specific self-concept KW - academic achievement KW - metacognition KW - executive functions KW - multilingual children KW - reading comprehension KW - reading fluency KW - spelling Y1 - 2019 U6 - https://doi.org/10.3390/bs9040039 SN - 2076-328X VL - 9 IS - 4 PB - MDPI AG CY - Basel ER - TY - JOUR A1 - Kameda, Takuya A1 - Zvick, Joel A1 - Vuk, Miriam A1 - Sadowska, Aleksandra A1 - Tam, Wai Kit A1 - Leung, Victor Y. A1 - Bölcskei, Kata A1 - Helyes, Zsuzsanna A1 - Applegate, Lee Ann A1 - Hausmann, Oliver N. A1 - Klasen, Juergen A1 - Krupkova, Olga A1 - Würtz-Kozak, Karin T1 - Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc BT - Association with Inflammation and Matrix Remodeling JF - International journal of molecular sciences N2 - Transient receptor potential (TRP) channels have emerged as potential sensors and transducers of inflammatory pain. The aims of this study were to investigate (1) the expression of TRP channels in intervertebral disc (IVD) cells in normal and inflammatory conditions and (2) the function of Transient receptor potential ankyrin 1 (TRPA1) and Transient receptor potential vanilloid 1 (TRPV1) in IVD inflammation and matrix homeostasis. RT-qPCR was used to analyze human fetal, healthy, and degenerated IVD tissues for the gene expression of TRPA1 and TRPV1. The primary IVD cell cultures were stimulated with either interleukin-1 beta (IL-1) or tumor necrosis factor alpha (TNF-) alone or in combination with TRPA1/V1 agonist allyl isothiocyanate (AITC, 3 and 10 mu M), followed by analysis of calcium flux and the expression of inflammation mediators (RT-qPCR/ELISA) and matrix constituents (RT-qPCR). The matrix structure and composition in caudal motion segments from TRPA1 and TRPV1 wild-type (WT) and knock-out (KO) mice was visualized by FAST staining. Gene expression of other TRP channels (A1, C1, C3, C6, V1, V2, V4, V6, M2, M7, M8) was also tested in cytokine-treated cells. TRPA1 was expressed in fetal IVD cells, 20% of degenerated IVDs, but not in healthy mature IVDs. TRPA1 expression was not detectable in untreated cells and it increased upon cytokine treatment, while TRPV1 was expressed and concomitantly reduced. In inflamed IVD cells, 10 mu M AITC activated calcium flux, induced gene expression of IL-8, and reduced disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) and collagen 1A1, possibly via upregulated TRPA1. TRPA1 KO in mice was associated with signs of degeneration in the nucleus pulposus and the vertebral growth plate, whereas TRPV1 KO did not show profound changes. Cytokine treatment also affected the gene expression of TRPV2 (increase), TRPV4 (increase), and TRPC6 (decrease). TRPA1 might be expressed in developing IVD, downregulated during its maturation, and upregulated again in degenerative disc disease, participating in matrix homeostasis. However, follow-up studies with larger sample sizes are needed to fully elucidate the role of TRPA1 and other TRP channels in degenerative disc disease. KW - low back pain KW - TRP channels KW - pro-inflammatory cytokines KW - aggrecanases KW - collagen KW - TRPA1 KW - TRPV1 KW - TRPV2 KW - TRPV4 KW - TRPC6 Y1 - 2019 U6 - https://doi.org/10.3390/ijms20071767 SN - 1422-0067 VL - 20 IS - 7 PB - MDPI CY - Basel ER - TY - JOUR A1 - Metref, Sammy A1 - Cosme, Emmanuel A1 - Le Sommer, Julien A1 - Poel, Nora A1 - Brankart, Jean-Michel A1 - Verron, Jacques A1 - Gomez Navarro, Laura T1 - Reduction of spatially structured errors in Wide-Swath altimetric satellite data using data assimilation JF - Remote sensing N2 - The Surface Water and Ocean Topography (SWOT) mission is a next generation satellite mission expected to provide a 2 km-resolution observation of the sea surface height (SSH) on a two-dimensional swath. Processing SWOT data will be challenging because of the large amount of data, the mismatch between a high spatial resolution and a low temporal resolution, and the observation errors. The present paper focuses on the reduction of the spatially structured errors of SWOT SSH data. It investigates a new error reduction method and assesses its performance in an observing system simulation experiment. The proposed error-reduction method first projects the SWOT SSH onto a subspace spanned by the SWOT spatially structured errors. This projection is removed from the SWOT SSH to obtain a detrended SSH. The detrended SSH is then processed within an ensemble data assimilation analysis to retrieve a full SSH field. In the latter step, the detrending is applied to both the SWOT data and an ensemble of model-simulated SSH fields. Numerical experiments are performed with synthetic SWOT observations and an ensemble from a North Atlantic, 1/60 degrees simulation of the ocean circulation (NATL60). The data assimilation analysis is carried out with an ensemble Kalman filter. The results are assessed with root mean square errors, power spectrum density, and spatial coherence. They show that a significant part of the large scale SWOT errors is reduced. The filter analysis also reduces the small scale errors and allows for an accurate recovery of the energy of the signal down to 25 km scales. In addition, using the SWOT nadir data to adjust the SSH detrending further reduces the errors. KW - SWOT KW - correlated errors KW - OSSE KW - projection KW - detrending KW - ensemble kalman filter Y1 - 2019 U6 - https://doi.org/10.3390/rs11111336 SN - 2072-4292 VL - 11 IS - 11 PB - MDPI CY - Basel ER - TY - JOUR A1 - Bolius, Sarah A1 - Wiedner, Claudia A1 - Weithoff, Guntram T1 - Low invasion success of an invasive cyanobacterium in a chlorophyte dominated lake JF - Scientific reports Y1 - 2019 SN - 2045-2322 VL - 9 PB - Macmillan Publishers Limited CY - London ER - TY - JOUR A1 - Feiner, Rebecca Christine A1 - Teschner, Julian A1 - Teschner, Kathrin E. A1 - Radukic, Marco T. A1 - Baumann, Tobias A1 - Hagen, Sven A1 - Hannappel, Yvonne A1 - Biere, Niklas A1 - Anselmetti, Dario A1 - Arndt, Katja Maren A1 - Müller, Kristian Mark T1 - rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations JF - International journal of molecular sciences N2 - Recombinant adeno-associated viruses (rAAV) provide outstanding options for customization and superior capabilities for gene therapy. To access their full potential, facile genetic manipulation is pivotal, including capsid loop modifications. Therefore, we assessed capsid tolerance to modifications of the structural VP proteins in terms of stability and plasticity. Flexible glycine-serine linkers of increasing sizes were, at the genetic level, introduced into the 587 loop region of the VP proteins of serotype 2, the best studied AAV representative. Analyses of biological function and thermal stability with respect to genome release of viral particles revealed structural plasticity. In addition, insertion of the 29 kDa enzyme beta-lactamase into the loop region was tested with a complete or a mosaic modification setting. For the mosaic approach, investigation of VP2 trans expression revealed that a Kozak sequence was required to prevent leaky scanning. Surprisingly, even the full capsid modification with beta-lactamase allowed for the assembly of capsids with a concomitant increase in size. Enzyme activity assays revealed lactamase functionality for both rAAV variants, which demonstrates the structural robustness of this platform technology. KW - adeno-associated-virus KW - beta-lactamase KW - inverted terminal repeat (ITR) KW - loop modification KW - capsid stability Y1 - 2019 U6 - https://doi.org/10.3390/ijms20225702 SN - 1422-0067 VL - 20 IS - 22 PB - MDPI CY - Basel ER - TY - JOUR A1 - Ayzel, Georgy A1 - Izhitskiy, Alexander T1 - Climate Change Impact Assessment on Freshwater Inflow into the Small Aral Sea JF - Water N2 - During the last few decades, the rapid separation of the Small Aral Sea from the isolated basin has changed its hydrological and ecological conditions tremendously. In the present study, we developed and validated the hybrid model for the Syr Darya River basin based on a combination of state-of-the-art hydrological and machine learning models. Climate change impact on freshwater inflow into the Small Aral Sea for the projection period 2007-2099 has been quantified based on the developed hybrid model and bias corrected and downscaled meteorological projections simulated by four General Circulation Models (GCM) for each of three Representative Concentration Pathway scenarios (RCP). The developed hybrid model reliably simulates freshwater inflow for the historical period with a Nash-Sutcliffe efficiency of 0.72 and a Kling-Gupta efficiency of 0.77. Results of the climate change impact assessment showed that the freshwater inflow projections produced by different GCMs are misleading by providing contradictory results for the projection period. However, we identified that the relative runoff changes are expected to be more pronounced in the case of more aggressive RCP scenarios. The simulated projections of freshwater inflow provide a basis for further assessment of climate change impacts on hydrological and ecological conditions of the Small Aral Sea in the 21st Century. KW - Small Aral Sea KW - hydrology KW - climate change KW - modeling KW - machine learning Y1 - 2019 U6 - https://doi.org/10.3390/w11112377 SN - 2073-4441 VL - 11 IS - 11 PB - MDPI CY - Basel ER - TY - JOUR A1 - Wu, Hao A1 - Han, Yijie A1 - Rodriguez Sillke, Yasmina A1 - Deng, Hongzhang A1 - Siddiqui, Sophiya A1 - Treese, Christoph A1 - Schmidt, Franziska A1 - Friedrich, Marie A1 - Keye, Jacqueline A1 - Wan, Jiajia A1 - Qin, Yue A1 - Kühl, Anja A. A1 - Qin, Zhihai A1 - Siegmund, Britta A1 - Glauben, Rainer T1 - Lipid droplet-dependent fatty acid metabolism controls the immune suppressive phenotype of tumor-associated macrophages JF - EMBO molecular medicine N2 - Tumor-associated macrophages (TAMs) promote tumor growth and metastasis by suppressing tumor immune surveillance. Herein, we provide evidence that the immunosuppressive phenotype of TAMs is controlled by long-chain fatty acid metabolism, specifically unsaturated fatty acids, here exemplified by oleate. Consequently, en-route enriched lipid droplets were identified as essential organelles, which represent effective targets for chemical inhibitors to block in vitro polarization of TAMs and tumor growth in vivo. In line, analysis of human tumors revealed that myeloid cells infiltrating colon cancer but not gastric cancer tissue indeed accumulate lipid droplets. Mechanistically, our data indicate that oleate-induced polarization of myeloid cells depends on the mammalian target of the rapamycin pathway. Thus, our findings reveal an alternative therapeutic strategy by targeting the pro-tumoral myeloid cells on a metabolic level. KW - cancer immunotherapy KW - lipid droplets KW - lipid metabolism KW - tumor microenvironment KW - tumor-associated macrophage Y1 - 2019 U6 - https://doi.org/10.15252/emmm.201910698 SN - 1757-4676 SN - 1757-4684 VL - 11 IS - 11 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Hsu, H. F. A1 - Krekhov, Andrey A1 - Tarantola, Marco A1 - Beta, Carsten A1 - Bodenschatz, Eberhardt T1 - Interplay between myosin II and actin dynamics in chemotactic amoeba JF - New journal of physics : the open-access journal for physics N2 - The actin cytoskeleton and its response to external chemical stimuli is fundamental to the mechano-biology of eukaryotic cells and their functions. One of the key players that governs the dynamics of the actin network is the motor protein myosin II. Based on a phase space embedding we have identified from experiments three phases in the cytoskeletal dynamics of starved Dictyostelium discoideum in response to a precisely controlled chemotactic stimulation. In the first two phases the dynamics of actin and myosin II in the cortex is uncoupled, while in the third phase the time scale for the recovery of cortical actin is determined by the myosin II dynamics. We report a theoretical model that captures the experimental observations quantitatively. The model predicts an increase in the optimal response time of actin with decreasing myosin II-actin coupling strength highlighting the role of myosin II in the robust control of cell contraction. KW - actin KW - myosin II KW - chemotaxis KW - oscillations KW - coupling KW - delay differential equation KW - contraction Y1 - 2019 U6 - https://doi.org/10.1088/1367-2630/ab5822 SN - 1367-2630 VL - 21 IS - 11 PB - IOP Publ. Ltd. CY - Bristol ER - TY - JOUR A1 - Kielar, Charlotte A1 - Xin, Yang A1 - Xu, Xiaodan A1 - Zhu, Siqi A1 - Gorin, Nelli A1 - Grundmeier, Guido A1 - Möser, Christin A1 - Smith, David M. A1 - Keller, Adrian T1 - Effect of staple age on DNA origami nanostructure assembly and stability JF - Molecules N2 - DNA origami nanostructures are widely employed in various areas of fundamental and applied research. Due to the tremendous success of the DNA origami technique in the academic field, considerable efforts currently aim at the translation of this technology from a laboratory setting to real-world applications, such as nanoelectronics, drug delivery, and biosensing. While many of these real-world applications rely on an intact DNA origami shape, they often also subject the DNA origami nanostructures to rather harsh and potentially damaging environmental and processing conditions. Furthermore, in the context of DNA origami mass production, the long-term storage of DNA origami nanostructures or their pre-assembled components also becomes an issue of high relevance, especially regarding the possible negative effects on DNA origami structural integrity. Thus, we investigated the effect of staple age on the self-assembly and stability of DNA origami nanostructures using atomic force microscopy. Different harsh processing conditions were simulated by applying different sample preparation protocols. Our results show that staple solutions may be stored at -20 degrees C for several years without impeding DNA origami self-assembly. Depending on DNA origami shape and superstructure, however, staple age may have negative effects on DNA origami stability under harsh treatment conditions. Mass spectrometry analysis of the aged staple mixtures revealed no signs of staple fragmentation. We, therefore, attribute the increased DNA origami sensitivity toward environmental conditions to an accumulation of damaged nucleobases, which undergo weaker base-pairing interactions and thus lead to reduced duplex stability. KW - DNA origami KW - atomic force microscopy KW - stability KW - storage Y1 - 2019 U6 - https://doi.org/10.3390/molecules24142577 SN - 1420-3049 VL - 24 IS - 14 PB - MDPI CY - Basel ER - TY - JOUR A1 - Yan, Wenhao A1 - Chen, Dijun A1 - Schumacher, Julia A1 - Durantini, Diego A1 - Engelhorn, Julia A1 - Chen, Ming A1 - Carles, Cristel C. A1 - Kaufmann, Kerstin T1 - Dynamic control of enhancer activity drives stage-specific gene expression during flower morphogenesis JF - Nature Communications N2 - Enhancers are critical for developmental stage-specific gene expression, but their dynamic regulation in plants remains poorly understood. Here we compare genome-wide localization of H3K27ac, chromatin accessibility and transcriptomic changes during flower development in Arabidopsis. H3K27ac prevalently marks promoter-proximal regions, suggesting that H3K27ac is not a hallmark for enhancers in Arabidopsis. We provide computational and experimental evidence to confirm that distal DNase. hypersensitive sites are predictive of enhancers. The predicted enhancers are highly stage-specific across flower development, significantly associated with SNPs for flowering-related phenotypes, and conserved across crucifer species. Through the integration of genome-wide transcription factor (TF) binding datasets, we find that floral master regulators and stage-specific TFs are largely enriched at developmentally dynamic enhancers. Finally, we show that enhancer clusters and intronic enhancers significantly associate with stage-specific gene regulation by floral master TFs. Our study provides insights into the functional flexibility of enhancers during plant development, as well as hints to annotate plant enhancers. Y1 - 2019 U6 - https://doi.org/10.1038/s41467-019-09513-2 SN - 2041-1723 VL - 10 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Schieferdecker, Anne A1 - Wendler, Petra T1 - Structural Mapping of Missense Mutations in the Pex1/Pex6 Complex JF - International journal of molecular sciences N2 - Peroxisome biogenesis disorders (PBDs) are nontreatable hereditary diseases with a broad range of severity. Approximately 65% of patients are affected by mutations in the peroxins Pex1 and Pex6. The proteins form the heteromeric Pex1/Pex6 complex, which is important for protein import into peroxisomes. To date, no structural data are available for this AAA+ ATPase complex. However, a wealth of information can be transferred from low-resolution structures of the yeast scPex1/scPex6 complex and homologous, well-characterized AAA+ ATPases. We review the abundant records of missense mutations described in PBD patients with the aim to classify and rationalize them by mapping them onto a homology model of the human Pex1/Pex6 complex. Several mutations concern functionally conserved residues that are implied in ATP hydrolysis and substrate processing. Contrary to fold destabilizing mutations, patients suffering from function-impairing mutations may not benefit from stabilizing agents, which have been reported as potential therapeutics for PBD patients. KW - Zellweger syndrome spectrum disorder (ZSSD) KW - Zellweger KW - structure KW - Pex1 KW - Pex6 KW - mutation Y1 - 2019 U6 - https://doi.org/10.3390/ijms20153756 SN - 1422-0067 VL - 20 IS - 15 PB - MDPI CY - Basel ER - TY - JOUR A1 - Martin-Lopez, Berta A1 - Leister, Ines A1 - Cruz, Pedro Lorenzo A1 - Palomo, Ignacio A1 - Gret-Regamey, Adrienne A1 - Harrison, Paula A. A1 - Lavorel, Sandra A1 - Locatelli, Bruno A1 - Luque, Sandra A1 - Walz, Ariane T1 - Nature’s contributions to people in mountains BT - a review JF - PLoS one N2 - Mountains play a key role in the provision of nature’s contributions to people (NCP) worldwide that support societies’ quality of life. Simultaneously, mountains are threatened by multiple drivers of change. Due to the complex interlinkages between biodiversity, quality of life and drivers of change, research on NCP in mountains requires interdisciplinary approaches. In this study, we used the conceptual framework of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) and the notion of NCP to determine to what extent previous research on ecosystem services in mountains has explored the different components of the IPBES conceptual framework. We conducted a systematic review of articles on ecosystem services in mountains published up to 2016 using the Web of Science and Scopus databases. Descriptive statistical and network analyses were conducted to explore the level of research on the components of the IPBES framework and their interactions. Our results show that research has gradually become more interdisciplinary by studying higher number of NCP, dimensions of quality of life, and indirect drivers of change. Yet, research focusing on biodiversity, regulating NCP and direct drivers has decreased over time. Furthermore, despite the fact that research on NCP in mountains becoming more policy-oriented over time, mainly in relation to payments for ecosystem services, institutional responses remained underexplored in the reviewed studies. Finally, we discuss the relevant knowledge gaps that should be addressed in future research in order to contribute to IPBES. Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0217847 SN - 1932-6203 VL - 14 IS - 6 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Hartlieb, Matthias A1 - Catrouillet, Sylvain A1 - Kuroki, Agnes A1 - Sanchez-Cano, Carlos A1 - Peltier, Raoul A1 - Perrier, Sebastien T1 - Stimuli-responsive membrane activity of cyclic-peptide-polymer conjugates JF - Chemical science N2 - Cyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT-polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membrane interaction using CPNT-polymer conjugates to permeabilize liposomes as well as mammalian cell membranes. Y1 - 2019 U6 - https://doi.org/10.1039/c9sc00756c SN - 2041-6520 SN - 2041-6539 VL - 10 IS - 21 SP - 5476 EP - 5483 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Cordonnier, Guillaume A1 - Bovy, Benoit A1 - Braun, Jean T1 - A versatile, linear complexity algorithm for flow routing in topographies with depressions JF - Earth surface dynamics N2 - We present a new algorithm for solving the common problem of flow trapped in closed depressions within digital elevation models, as encountered in many applications relying on flow routing. Unlike other approaches (e.g., the Priority-Flood depression filling algorithm), this solution is based on the explicit computation of the flow paths both within and across the depressions through the construction of a graph connecting together all adjacent drainage basins. Although this represents many operations, a linear time complexity can be reached for the whole computation, making it very efficient. Compared to the most optimized solutions proposed so far, we show that this algorithm of flow path enforcement yields the best performance when used in landscape evolution models. In addition to its efficiency, our proposed method also has the advantage of letting the user choose among different strategies of flow path enforcement within the depressions (i.e., filling vs. carving). Furthermore, the computed graph of basins is a generic structure that has the potential to be reused for solving other problems as well, such as the simulation of erosion. This sequential algorithm may be helpful for those who need to, e.g., process digital elevation models of moderate size on single computers or run batches of simulations as part of an inference study. Y1 - 2019 U6 - https://doi.org/10.5194/esurf-7-549-2019 SN - 2196-6311 SN - 2196-632X VL - 7 IS - 2 SP - 549 EP - 562 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Chaturvedi, Neha A1 - Mehrotra, Bagish A1 - Kumari, Sangeeta A1 - Gupta, Saurabh A1 - Subramanya, Hosahalli A1 - Saberwal, Gayatri T1 - Some data quality issues at ClinicalTrials.gov JF - Trials KW - ClinicalTrials KW - gov KW - Drugs KW - Biologicals KW - Clinical trial KW - Principal Investigator KW - Data quality KW - Database errors Y1 - 2019 U6 - https://doi.org/10.1186/s13063-019-3408-2 SN - 1745-6215 VL - 20 PB - BMC CY - London ER - TY - JOUR A1 - Brand, Ralf A1 - Cheval, Boris T1 - Theories to explain exercise motivation and physical inactivity BT - ways of expanding our current theoretical perspective JF - Frontiers in psychology KW - exercise KW - motivation KW - affect KW - automaticity KW - physical inactivity Y1 - 2019 U6 - https://doi.org/10.3389/fpsyg.2019.01147 SN - 1664-1078 VL - 10 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Ringel, Lisa Maria A1 - Somogyvári, Márk A1 - Jalali, Mohammadreza A1 - Bayer, Peter T1 - Comparison of hydraulic and tracer tomography for discrete fracture network inversion JF - Geosciences N2 - Fractures serve as highly conductive preferential flow paths for fluids in rocks, which are difficult to exactly reconstruct in numerical models. Especially, in low-conductive rocks, fractures are often the only pathways for advection of solutes and heat. The presented study compares the results from hydraulic and tracer tomography applied to invert a theoretical discrete fracture network (DFN) that is based on data from synthetic cross-well testing. For hydraulic tomography, pressure pulses in various injection intervals are induced and the pressure responses in the monitoring intervals of a nearby observation well are recorded. For tracer tomography, a conservative tracer is injected in different well levels and the depth-dependent breakthrough of the tracer is monitored. A recently introduced transdimensional Bayesian inversion procedure is applied for both tomographical methods, which adjusts the fracture positions, orientations, and numbers based on given geometrical fracture statistics. The used Metropolis-Hastings-Green algorithm is refined by the simultaneous estimation of the measurement error’s variance, that is, the measurement noise. Based on the presented application to invert the two-dimensional cross-section between source and the receiver well, the hydraulic tomography reveals itself to be more suitable for reconstructing the original DFN. This is based on a probabilistic representation of the inverted results by means of fracture probabilities. KW - hydraulic tomography KW - tracer tomography KW - DFN KW - Bayesian inversion KW - heterogeneity KW - fracture KW - hydrogeophysics Y1 - 2019 U6 - https://doi.org/10.3390/geosciences9060274 SN - 2076-3263 VL - 9 IS - 6 PB - MDPI CY - Basel ER - TY - JOUR A1 - Romanowsky, Erik A1 - Handorf, Dörthe A1 - Jaiser, Ralf A1 - Wohltmann, Ingo A1 - Dorn, Wolfgang A1 - Ukita, Jinro A1 - Cohen, Judah A1 - Dethloff, Klaus A1 - Rex, Markus T1 - The role of stratospheric ozone for Arctic-midlatitude linkages JF - Scientific reports N2 - Arctic warming was more pronounced than warming in midlatitudes in the last decades making this region a hotspot of climate change. Associated with this, a rapid decline of sea-ice extent and a decrease of its thickness has been observed. Sea-ice retreat allows for an increased transport of heat and momentum from the ocean up to the tropo- and stratosphere by enhanced upward propagation of planetary-scale atmospheric waves. In the upper atmosphere, these waves deposit the momentum transported, disturbing the stratospheric polar vortex, which can lead to a breakdown of this circulation with the potential to also significantly impact the troposphere in mid- to late-winter and early spring. Therefore, an accurate representation of stratospheric processes in climate models is necessary to improve the understanding of the impact of retreating sea ice on the atmospheric circulation. By modeling the atmospheric response to a prescribed decline in Arctic sea ice, we show that including interactive stratospheric ozone chemistry in atmospheric model calculations leads to an improvement in tropo-stratospheric interactions compared to simulations without interactive chemistry. This suggests that stratospheric ozone chemistry is important for the understanding of sea ice related impacts on atmospheric dynamics. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-43823-1 SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Kayhan, Ezgi A1 - Heil, Lieke A1 - Kwisthout, Johan A1 - van Rooij, Iris A1 - Hunnius, Sabine A1 - Bekkering, Harold T1 - Young children integrate current observations, priors and agent information to predict others’ actions JF - PLOS ONE / Public Library of Science N2 - From early on in life, children are able to use information from their environment to form predictions about events. For instance, they can use statistical information about a population to predict the sample drawn from that population and infer an agent’s preferences from systematic violations of random sampling. We investigated whether and how young children infer an agent’s sampling biases. Moreover, we examined whether pupil data of toddlers follow the predictions of a computational model based on the causal Bayesian network formalization of predictive processing. We formalized three hypotheses about how different explanatory variables (i.e., prior probabilities, current observations, and agent characteristics) are used to predict others’ actions. We measured pupillary responses as a behavioral marker of ‘prediction errors’ (i.e., the perceived mismatch between what one’s model of an agent predicts and what the agent actually does). Pupillary responses of 24-month-olds, but not 18-month-olds, showed that young children integrated information about current observations, priors and agents to make predictions about agents and their actions. These findings shed light on the mechanisms behind toddlers’ inferences about agent-caused events. To our knowledge, this is the first study in which young children's pupillary responses are used as markers of prediction errors, which were qualitatively compared to the predictions by a computational model based on the causal Bayesian network formalization of predictive processing. Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0200976 SN - 1932-6203 VL - 14 IS - 5 PB - PLOS CY - San Fransisco ER - TY - JOUR A1 - Frommhold, Martin A1 - Heim, Arend A1 - Barabanov, Mikhail A1 - Maier, Franziska A1 - Mühle, Ralf-Udo A1 - Smirenski, Sergei M. A1 - Heim, Wieland T1 - Breeding habitat and nest-site selection by an obligatory "nest-cleptoparasite", the Amur Falcon Falco amurensis JF - Ecology and evolution N2 - The selection of a nest site is crucial for successful reproduction of birds. Animals which re-use or occupy nest sites constructed by other species often have limited choice. Little is known about the criteria of nest-stealing species to choose suitable nesting sites and habitats. Here, we analyze breeding-site selection of an obligatory "nest-cleptoparasite", the Amur Falcon Falco amurensis. We collected data on nest sites at Muraviovka Park in the Russian Far East, where the species breeds exclusively in nests of the Eurasian Magpie Pica pica. We sampled 117 Eurasian Magpie nests, 38 of which were occupied by Amur Falcons. Nest-specific variables were assessed, and a recently developed habitat classification map was used to derive landscape metrics. We found that Amur Falcons chose a wide range of nesting sites, but significantly preferred nests with a domed roof. Breeding pairs of Eurasian Hobby Falco subbuteo and Eurasian Magpie were often found to breed near the nest in about the same distance as neighboring Amur Falcon pairs. Additionally, the occurrence of the species was positively associated with bare soil cover, forest cover, and shrub patches within their home range and negatively with the distance to wetlands. Areas of wetlands and fallow land might be used for foraging since Amur Falcons mostly depend on an insect diet. Additionally, we found that rarely burned habitats were preferred. Overall, the effect of landscape variables on the choice of actual nest sites appeared to be rather small. We used different classification methods to predict the probability of occurrence, of which the Random forest method showed the highest accuracy. The areas determined as suitable habitat showed a high concordance with the actual nest locations. We conclude that Amur Falcons prefer to occupy newly built (domed) nests to ensure high nest quality, as well as nests surrounded by available feeding habitats. KW - cleptoparasitism KW - fire KW - habitat use KW - machine learning KW - magpie KW - nest-site selection KW - random forest Y1 - 2019 U6 - https://doi.org/10.1002/ece3.5878 SN - 2045-7758 VL - 9 IS - 24 SP - 14430 EP - 14441 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Eilers, Elisabeth Johanna A1 - Heger, Tina T1 - Past Competition Affects Offspring Foliar Terpenoid Concentrations, Seed Traits, and Fitness in the Invasive Forb Erodium cicutarium (Geraniaceae) JF - Frontiers in Ecology and Evolution N2 - (1) Environmental conditions experienced in the past may lead to intraspecific differences in ecological and chemical traits of plants, which likely affect future responses to altered or new environments. Whether competition by neighbors is such a trait-shaping factor is not yet well-known. We aimed to understand how the level of ancestral plant competition affects traits related to plant fitness and resource allocation, reproduction, and (phyto-)toxin accumulation in offspring, and whether a potential differentiation in these traits can be found in different geographic origins of which one belongs to the native and one to the invaded range. (2) We compared differentiation of the following traits in offspring plants of multiple populations in Erodium cicutarium (Geraniaceae): biomass, seed production, seed traits related to dispersal and germination, and concentrations of foliar mono- and sesquiterpenes. We tested the allelopatic potential of aqueous extracts of the same E. cicutarium plants on seeds of five different plant families. (3) In plants originating from populations that experienced high levels of competition, we found twice as high monoterpene concentrations. These plants also produced more biomass and a higher proportion of ripe to unripe seeds until harvesting. Seeds originating from high competition sites were shorter. Aqueous E. cicutarium leaf extracts with high terpenoid content reduced radicle length of Zea mays and radicle and hypocotyl length of E. cicutarium seedlings. (4) The results of this study provide first evidence that the surrounding vegetation may shape chemo-ecological plant traits that may be fundamental for competitive ability. Our study calls for more research testing whether competition experienced in the native range may lead to an enhanced capability of plants to establish populations and spread in a new range. KW - resource allocation KW - specialized metabolites KW - gas chromatography-mass spectrometry KW - seed morphology KW - plant interactions KW - eco-evolutionary experience Y1 - 2019 U6 - https://doi.org/10.3389/fevo.2019.00392 SN - 2296-701X VL - 7 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Masson, Torsten A1 - Bamberg, Sebastian A1 - Stricker, Michael A1 - Heidenreich, Anna T1 - "We can help ourselves": does community resilience buffer against the negative impact of flooding on mental health? JF - Natural hazards and earth system sciences N2 - Empirical evidence of the relationship between social support and post-disaster mental health provides support for a general beneficial effect of social support (main-effect model; Wheaton, 1985). From a theoretical perspective, a buffering effect of social support on the negative relationship between disaster-related stress and mental health also seems plausible (stress-buffering model; Wheaton, 1985). Previous studies, however, (a) have paid less attention to the buffering effect of social support and (b) have mainly relied on interpersonal support (but not collective-level support such as community resilience) when investigating this issue. This previous work might have underestimated the effect of support on post-disaster mental health. Building on a sample of residents in Germany recently affected by flooding (N = 118), we show that community resilience to flooding (but not general interpersonal social support) buffered against the negative effects of flooding on post-disaster mental health. The results support the stress-buffering model and call for a more detailed look at the relationship between support and resilience and post-disaster adjustment, including collective-level variables. Y1 - 2019 U6 - https://doi.org/10.5194/nhess-19-2371-2019 SN - 1561-8633 SN - 1684-9981 VL - 19 IS - 11 SP - 2371 EP - 2384 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Totz, Sonja Juliana A1 - Petri, Stefan A1 - Lehmann, Jascha A1 - Peukert, Erik A1 - Coumou, Dim T1 - Exploring the sensitivity of Northern Hemisphere atmospheric circulation to different surface temperature forcing using a statistical-dynamical atmospheric model JF - Nonlinear processes in geophysics N2 - Climate and weather conditions in the mid-latitudes are strongly driven by the large-scale atmosphere circulation. Observational data indicate that important components of the large-scale circulation have changed in recent decades, including the strength and the width of the Hadley cell, jets, storm tracks and planetary waves. Here, we use a new statistical-dynamical atmosphere model (SDAM) to test the individual sensitivities of the large-scale atmospheric circulation to changes in the zonal temperature gradient, meridional temperature gradient and global-mean temperature. We analyze the Northern Hemisphere Hadley circulation, jet streams, storm tracks and planetary waves by systematically altering the zonal temperature asymmetry, the meridional temperature gradient and the global-mean temperature. Our results show that the strength of the Hadley cell, storm tracks and jet streams depend, in terms of relative changes, almost linearly on both the global-mean temperature and the meridional temperature gradient, whereas the zonal temperature asymmetry has little or no influence. The magnitude of planetary waves is affected by all three temperature components, as expected from theoretical dynamical considerations. The width of the Hadley cell behaves nonlinearly with respect to all three temperature components in the SDAM. Moreover, some of these observed large-scale atmospheric changes are expected from dynamical equations and are therefore an important part of model validation. Y1 - 2019 U6 - https://doi.org/10.5194/npg-26-1-2019 SN - 1023-5809 SN - 1607-7946 VL - 26 IS - 1 SP - 1 EP - 12 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Gailleton, Boris A1 - Mudd, Simon M. A1 - Clubb, Fiona J. A1 - Peifer, Daniel A1 - Hurst, Martin D. T1 - A segmentation approach for the reproducible extraction and quantification of knickpoints from river long profiles JF - Earth surface dynamics N2 - Changes in the steepness of river profiles or abrupt vertical steps (i.e. waterfalls) are thought to be indicative of changes in erosion rates, lithology or other factors that affect landscape evolution. These changes are referred to as knickpoints or knickzones and are pervasive in bedrock river systems. Such features are thought to reveal information about landscape evolution and patterns of erosion, and therefore their locations are often reported in the geomorphic literature. It is imperative that studies reporting knickpoints and knickzones use a reproducible method of quantifying their locations, as their number and spatial distribution play an important role in interpreting tectonically active landscapes. In this contribution we introduce a reproducible knickpoint and knickzone extraction algorithm that uses river profiles transformed by integrating drainage area along channel length (the so-called integral or chi method). The profile is then statistically segmented and the differing slopes and step changes in the elevations of these segments are used to identify knickpoints, knickzones and their relative magnitudes. The output locations of identified knickpoints and knickzones compare favourably with human mapping: we test the method on Santa Cruz Island, CA, using previously reported knickzones and also test the method against a new dataset from the Quadrilatero Ferrifero in Brazil. The algorithm allows for the extraction of varying knickpoint morphologies, including stepped, positive slope-break (concave upward) and negative slope-break knickpoints. We identify parameters that most affect the resulting knickpoint and knickzone locations and provide guidance for both usage and outputs of the method to produce reproducible knickpoint datasets. Y1 - 2019 U6 - https://doi.org/10.5194/esurf-7-211-2019 SN - 2196-6311 SN - 2196-632X VL - 7 IS - 1 SP - 211 EP - 230 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Gou, Tingyu A1 - Liu, Rui A1 - Kliem, Bernhard A1 - Wang, Yuming A1 - Veronig, Astrid M. T1 - The birth of a coronal mass ejection JF - Science Advances N2 - The Sun’s atmosphere is frequently disrupted by coronal mass ejections (CMEs), coupled with flares and energetic particles. The coupling is usually attributed to magnetic reconnection at a vertical current sheet connecting the flare and CME, with the latter embedding a helical magnetic structure known as flux rope. However, both the origin of flux ropes and their nascent paths toward eruption remain elusive. Here, we present an observation of how a stellar-sized CME bubble evolves continuously from plasmoids, mini flux ropes that are barely resolved, within half an hour. The eruption initiates when plasmoids springing from a vertical current sheet merge into a leading plasmoid, which rises at increasing speeds and expands impulsively into the CME bubble, producing hard x-ray bursts simultaneously. This observation illuminates a complete CME evolutionary path capable of accommodating a wide variety of plasma phenomena by bridging the gap between microscale and macroscale dynamics. Y1 - 2019 U6 - https://doi.org/10.1126/sciadv.aau7004 SN - 2375-2548 VL - 5 IS - 3 PB - American Assoc. for the Advancement of Science CY - Washington ER - TY - JOUR A1 - Rodriguez-Zuluaga, Juan A1 - Stolle, Claudia T1 - Interhemispheric field-aligned currents at the edges of equatorial plasma depletions JF - Scientific reports N2 - A comprehensive description of electromagnetic processes related to equatorial plasma depletions (EPDs) is essential for understanding their evolution and day-to-day variability. Recently, field-aligned currents (FACs) flowing at both western and eastern edges of EPDs were observed to be interhemispheric rather than anti-parallel about the dip equator, as suggested by previous theoretical studies. In this paper, we investigate the spatial and temporal variability of the FACs orientation using simultaneous measurements of electron density and magnetic field gathered by ESA’s Swarm constellation mission. By using empirical models, we assess the role of the Pedersen conductance in the preference of the FACs to close either in the northern or southern magnetic hemisphere. Here we show that the closure of the FACs agrees with an electrostatic regime determined by a hemispherical asymmetry of the Pedersen conductance. That is, the EPD-related FACs close at lower altitudes in the hemisphere with the highest conductivity. The evidence of this conclusion stands on the general agreement between the longitudinal and seasonal variability of both the conductivity and the FACs orientation. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-018-37955-z SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Nagel, Oliver A1 - Frey, Manuel A1 - Gerhardt, Matthias A1 - Beta, Carsten T1 - Harnessing Motile Amoeboid Cells as Trucks for Microtransport and -Assembly JF - Advanced science N2 - Cell-driven microtransport is one of the most prominent applications in the emerging field of biohybrid systems. While bacterial cells have been successfully employed to drive the swimming motion of micrometer-sized cargo particles, the transport capacities of motile adherent cells remain largely unexplored. Here, it is demonstrated that motile amoeboid cells can act as efficient and versatile trucks to transport microcargo. When incubated together with microparticles, cells of the social amoeba Dictyostelium discoideum readily pick up and move the cargo particles. Relying on the unspecific adhesive properties of the amoeba, a wide range of different cargo materials can be used. The cell-driven transport can be directionally guided based on the chemotactic responses of amoeba to chemoattractant gradients. On the one hand, the cargo can be assembled into clusters in a self-organized fashion, relying on the developmentally induced chemotactic aggregation of cells. On the other hand, chemoattractant gradients can be externally imposed to guide the cellular microtrucks to a desired location. Finally, larger cargo particles of different shapes that exceed the size of a single cell by more than an order of magnitude, can also be transported by the collective effort of large numbers of motile cells. KW - biohybrid microsystems KW - chemotaxis KW - Dictyostelium discoideum KW - microtransport and -assembly Y1 - 2018 U6 - https://doi.org/10.1002/advs.201801242 SN - 2198-3844 VL - 6 IS - 3 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Czapka, Sophia A1 - Klassert, Annegret A1 - Festman, Julia T1 - Executive functions and language BT - their differential influence on mono- vs. multilingual spelling in primary school JF - Frontiers in psychology N2 - We aimed at unveiling the role of executive functions (EFs) and language-related skills in spelling for mono- versus multilingual primary school children. We focused on EF and language-related skills, in particular lexicon size and phonological awareness (PA), because these factors were found to predict spelling in studies predominantly conducted with monolinguals, and because multilingualism can modulate these factors. There is evidence for (a) a bilingual advantage in EF due to constant high cognitive demands through language control, (b) a smaller mental lexicon in German and (c) possibly better PA. Multilinguals in Germany show on average poorer German language proficiency, what can influence performance on language-based tasks negatively. Thus, we included two spelling tasks to tease apart spelling based on lexical knowledge (i.e., word spelling) from spelling based on non-lexical strategies (i.e., non-word spelling). Our sample consisted of heterogeneous third graders from Germany: 69 monolinguals (age: M = 108 months) and 57 multilinguals (age: M = 111 months). On less language-dependent tasks (e.g., non-word spelling, PA, intelligence, short-term memory (STM) and three EF tasks testing switching, inhibition, and working memory) performance of both groups did not differ significantly. However, multilinguals performed significantly more poorly on tasks measuring German lexicon size and word spelling than monolinguals. Regression analyses revealed that for multilinguals, inhibition was related to spelling, whereas switching was the only EF component to influence word spelling in monolinguals and non-word spelling performance in both groups. By adding lexicon size and other language-related factors to the regression models, the influence of switching was reduced to insignificant effects, but inhibition remained significant for multilinguals. Language-related skills best predicted spelling and both language groups shared those variables: PA for word spelling, and STM for non-word spelling. Additionally, multilinguals’ word spelling performance was also predicted by their German lexicon size, and non-word spelling performance by PA. This study offers an in-depth look at spelling acquisition at a certain point of literacy development. Mono- and multilinguals have the predominant factors for spelling in common, but probably due to superior language knowledge, monolinguals were already able to make use of EF during spelling. For multilinguals, German lexicon size was more important for spelling than EF. For multilinguals’ spelling these functions might come into play only at a later stage. KW - bilingualism KW - spelling KW - literacy acquisition KW - executive functions KW - lexicon size KW - primary school Y1 - 2019 U6 - https://doi.org/10.3389/fpsyg.2019.00097 SN - 1664-1078 VL - 10 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Hadad, Yemima T1 - Hasidic Myth-Activism BT - Martin Buber’s Theopolitical Revision of Volkish Nationalism JF - Religions N2 - Since the 1970s, Buber has often been suspected of being a Volkish thinker. This essay reconsiders the affinity of Buber’s late writings with Volkish ideology. It examines the allegations against Buber’s Volkish thought in light of his later biblical and Hasidic writings. By illuminating the ideological affinity between these two modes of thought, the essay explains how Buber aims to depart from the dangers of myth without rejecting myth as such. I argue that Buber’s relationship to myth can help us to explain his critique of nationalism. My basic argument is that in his struggle with hyper-nationalism, Buber follows the Baal Shem Tov and his struggle against Sabbateanism. Like the Besht, Buber does not reject myth, but seeks instead to repair it from within. Whereas hyper-nationalism uses myth to advance its political goals, Buber seeks to reposition ethics within a mythic framework. I view Buber’s exegesis and commentaries on biblical and Hasidic myths as myth-activism. KW - Myth-Activism KW - Martin Buber KW - theopolitics KW - Zionism KW - Hasidism KW - myth KW - activism KW - Volkism KW - Judaism KW - politics Y1 - 2019 U6 - https://doi.org/10.3390/rel10020096 SN - 2077-1444 VL - 10 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Sowemimo, Oluwakemi T. A1 - Knox-Brown, Patrick A1 - Borcherds, Wade A1 - Rindfleisch, Tobias A1 - Thalhammer, Anja A1 - Daughdrill, Gary W. T1 - Conserved Glycines Control Disorder and Function in the Cold-Regulated Protein, COR15A JF - Biomolecules N2 - Cold-regulated (COR) 15A is an intrinsically disordered protein (IDP) from Arabidopsis thaliana important for freezing tolerance. During freezing-induced cellular dehydration, COR15A transitions from a disordered to mostly alpha-helical structure. We tested whether mutations that increase the helicity of COR15A also increase its protective function. Conserved glycine residues were identified and mutated to alanine. Nuclear magnetic resonance (NMR) spectroscopy was used to identify residue-specific changes in helicity for wildtype (WT) COR15A and the mutants. Circular dichroism (CD) spectroscopy was used to monitor the coil-helix transition in response to increasing concentrations of trifluoroethanol (TFE) and ethylene glycol. The impact of the COR15A mutants on the stability of model membranes during a freeze-thaw cycle was investigated by fluorescence spectroscopy. The results of these experiments showed the mutants had a higher content of alpha-helical structure and the increased alpha-helicity improved membrane stabilization during freezing. Comparison of the TFE- and ethylene glycol-induced coil-helix transitions support our conclusion that increasing the transient helicity of COR15A in aqueous solution increases its ability to stabilize membranes during freezing. Altogether, our results suggest the conserved glycine residues are important for maintaining the disordered structure of COR15A but are also compatible with the formation of alpha-helical structure during freezing induced dehydration. KW - COR15A KW - Late embryogenesis abundant KW - intrinsically disordered proteins KW - Trifluoroethanol KW - Nuclear magnetic resonance Y1 - 2019 U6 - https://doi.org/10.3390/biom9030084 SN - 2218-273X VL - 9 IS - 3 PB - MDPI CY - Basel ER - TY - JOUR A1 - Bubner, Ben A1 - Buchheit, Ramona A1 - Friedrich, Frank A1 - Kummer, Volker A1 - Scholler, Markus T1 - Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov. JF - MycoKeys N2 - Species of rust fungi of the genus Milesina (Pucciiastraceae, Pucciniales) are distributed mainly in northern temperate regions. They host-alternate between needles of fir (Abies spp.) and fronds of ferns (species of Polypodiales). Milesina species are distinguished based on host taxonomy and urediniospore morphology. In this study, 12 species of Milesina from Europe were revised. Specimens were examined by light and scanning electron microscopy for urediniospore morphology with a focus on visualising germ pores (number, size and position) and echinulation. In addition, barcode loci (ITS, nad6, 28S) were used for species delimitation and for molecular phylogenetic analyses. Barcodes of 72 Milesina specimens were provided, including 11 of the 12 species. Whereas urediniospore morphology features were sufficient to distinguish all 12 Milesina species except for 2 (M. blechni and M. kriegeriana), ITS sequences separated only 4 of 11 species. Sequencing with 28S and nad6 did not improve species resolution. Phylogenetic analysis, however, revealed four phylogenetic groups within Milesina that also correlate with specific urediniospore characters (germ pore number and position and echinulation). These groups are proposed as new sections within Milesina (sections Milesina, Vogesiacae M. Scholler & Bubner, sect. nov., Scolopendriorum M. Scholler & Bubner, sect. nov. and Carpaticae M. Scholler & Bubner, sect. nov.). In addition, Milesina woodwardiana Buchheit & M. Scholler, sp. nov. on Woodwardia radicans, a member of the type section Milesina, is newly described. An identification key for European Milesina species, based on urediniospore features, is provided. KW - Abies alba KW - Polypodiales KW - GBOL KW - germ pores KW - host alternation KW - Uredinopsis KW - Europe Y1 - 2019 U6 - https://doi.org/10.3897/mycokeys.48.30350 SN - 1314-4057 SN - 1314-4049 IS - 48 SP - 1 EP - 40 PB - Pensoft Publishers CY - Sofia ER - TY - JOUR A1 - Schoonover, Heather A. A1 - Gret-Regamey, Adrienne A1 - Metzger, Marc J. A1 - Ruiz-Frau, Ana A1 - Santos-Reis, Margarida A1 - Scholte, Samantha S. K. A1 - Walz, Ariane A1 - Nicholas, Kimberly A. T1 - Creating space, aligning motivations, and building trust BT - a practical framework for stakeholder engagement based on experience in 12 ecosystem services case studies JF - Ecology and society : a journal of integrative science for resilience and sustainability N2 - Ecosystem services inherently involve people, whose values help define the benefits of nature's services. It is thus important for researchers to involve stakeholders in ecosystem services research. However, a simple and practicable framework to guide such engagement, and in particular to help researchers anticipate and consider key issues and challenges, has not been well explored. Here, we use experience from the 12 case studies in the European Operational Potential of Ecosystem Research Applications (OPERAs) project to propose a stakeholder engagement framework comprising three key elements: creating space, aligning motivations, and building trust. We argue that involving stakeholders in research demands thoughtful reflection from the researchers about what kind of space they want to create, including if and how they want to bring different interests together, how much space they want to allow for critical discussion, and whether there is a role for particular stakeholders to serve as conduits between others. In addition, understanding their own motivations—including values, knowledge, goals, and desired benefits—will help researchers decide when and how to involve stakeholders, identify areas of common ground and potential disagreement, frame the project appropriately, set expectations, and ensure each party is able to see benefits of engaging with each other. Finally, building relationships with stakeholders can be difficult but considering the roles of existing relationships, time, approach, reputation, and belonging can help build mutual trust. Although the three key elements and the paths between them can play out differently depending on the particular research project, we suggest that a research design that considers how to create the space in which researchers and stakeholders will meet, align motivations between researchers and stakeholders, and build mutual trust will help foster productive researcher–stakeholder relationships. KW - cocreated knowledge KW - ecosystem services KW - participatory research KW - research design KW - stakeholder engagement KW - transdisciplinary research Y1 - 2019 U6 - https://doi.org/10.5751/ES-10061-240111 SN - 1708-3087 VL - 24 IS - 1 PB - Resilience Alliance CY - Wolfville ER - TY - JOUR A1 - Reschke, Maria A1 - Rehfeld, Kira A1 - Laepple, Thomas T1 - Empirical estimate of the signal content of Holocene temperature proxy records JF - Climate of the past : an interactive open access journal of the European Geosciences Union N2 - Proxy records from climate archives provide evidence about past climate changes, but the recorded signal is affected by non-climate-related effects as well as time uncertainty. As proxy-based climate reconstructions are frequently used to test climate models and to quantitatively infer past climate, we need to improve our understanding of the proxy record signal content as well as the uncertainties involved. In this study, we empirically estimate signal-to-noise ratios (SNRs) of temperature proxy records used in global compilations of the middle to late Holocene (last 6000 years). This is achieved through a comparison of the correlation of proxy time series from nearby sites of three compilations and model time series extracted at the proxy sites from two transient climate model simulations: a Holocene simulation of the ECHAM5/MPI-OM model and the Holocene part of the TraCE-21ka simulation. In all comparisons, we found the mean correlations of the proxy time series on centennial to millennial timescales to be low (R < 0.2), even for nearby sites, which resulted in low SNR estimates. The estimated SNRs depend on the assumed time uncertainty of the proxy records, the timescale analysed, and the model simulation used. Using the spatial correlation structure of the ECHAM5/MPI-OM simulation, the estimated SNRs on centennial timescales ranged from 0.05 - assuming no time uncertainty - to 0.5 for a time uncertainty of 400 years. On millennial timescales, the estimated SNRs were generally higher. Use of the TraCE-21ka correlation structure generally resulted in lower SNR estimates than for ECHAM5/MPI-OM. As the number of available high-resolution proxy records continues to grow, a more detailed analysis of the signal content of specific proxy types should become feasible in the near future. The estimated low signal content of Holocene temperature compilations should caution against over-interpretation of these multi-proxy and multisite syntheses until further studies are able to facilitate a better characterisation of the signal content in paleoclimate records. Y1 - 2019 U6 - https://doi.org/10.5194/cp-15-521-2019 SN - 1814-9324 SN - 1814-9332 VL - 15 IS - 2 SP - 521 EP - 537 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Krapf, Diego A1 - Lukat, Nils A1 - Marinari, Enzo A1 - Metzler, Ralf A1 - Oshanin, Gleb A1 - Selhuber-Unkel, Christine A1 - Squarcini, Alessio A1 - Stadler, Lorenz A1 - Weiss, Matthias A1 - Xu, Xinran T1 - Spectral Content of a Single Non-Brownian Trajectory JF - Physical review : X, Expanding access N2 - Time-dependent processes are often analyzed using the power spectral density (PSD) calculated by taking an appropriate Fourier transform of individual trajectories and finding the associated ensemble average. Frequently, the available experimental datasets are too small for such ensemble averages, and hence, it is of a great conceptual and practical importance to understand to which extent relevant information can be gained from S(f, T), the PSD of a single trajectory. Here we focus on the behavior of this random, realization-dependent variable parametrized by frequency f and observation time T, for a broad family of anomalous diffusions-fractional Brownian motion with Hurst index H-and derive exactly its probability density function. We show that S(f, T) is proportional-up to a random numerical factor whose universal distribution we determine-to the ensemble-averaged PSD. For subdiffusion (H < 1/2), we find that S(f, T) similar to A/f(2H+1) with random amplitude A. In sharp contrast, for superdiffusion (H > 1/2) S(f, T) similar to BT2H-1/f(2) with random amplitude B. Remarkably, for H > 1/2 the PSD exhibits the same frequency dependence as Brownian motion, a deceptive property that may lead to false conclusions when interpreting experimental data. Notably, for H > 1/2 the PSD is ageing and is dependent on T. Our predictions for both sub-and superdiffusion are confirmed by experiments in live cells and in agarose hydrogels and by extensive simulations. KW - Biological Physics KW - Interdisciplinary Physics KW - Statistical Physics Y1 - 2019 U6 - https://doi.org/10.1103/PhysRevX.9.011019 SN - 2160-3308 VL - 9 IS - 1 PB - American Physical Society CY - College Park ER - TY - JOUR A1 - Muster, Sina A1 - Riley, William J. A1 - Roth, Kurt A1 - Langer, Moritz A1 - Aleina, Fabio Cresto A1 - Koven, Charles D. A1 - Lange, Stephan A1 - Bartsch, Annett A1 - Grosse, Guido A1 - Wilson, Cathy J. A1 - Jones, Benjamin M. A1 - Boike, Julia T1 - Size distributions of arctic waterbodies reveal consistent relations in their statistical moments in space and time JF - Frontiers in Earth Science N2 - Arctic lowlands are characterized by large numbers of small waterbodies, which are known to affect surface energy budgets and the global carbon cycle. Statistical analysis of their size distributions has been hindered by the shortage of observations at sufficiently high spatial resolutions. This situation has now changed with the high-resolution (<5 m) circum-Arctic Permafrost Region Pond and Lake (PeRL) database recently becoming available. We have used this database to make the first consistent, high-resolution estimation of Arctic waterbody size distributions, with surface areas ranging from 0.0001 km(2) (100 m(2)) to 1 km(2). We found that the size distributions varied greatly across the thirty study regions investigated and that there was no single universal size distribution function (including power-law distribution functions) appropriate across all of the study regions. We did, however, find close relationships between the statistical moments (mean, variance, and skewness) of the waterbody size distributions from different study regions. Specifically, we found that the spatial variance increased linearly with mean waterbody size (R-2 = 0.97, p < 2.2e-16) and that the skewness decreased approximately hyperbolically. We have demonstrated that these relationships (1) hold across the 30 Arctic study regions covering a variety of (bio)climatic and permafrost zones, (2) hold over time in two of these study regions for which multi-decadal satellite imagery is available, and (3) can be reproduced by simulating rising water levels in a high-resolution digital elevation model. The consistent spatial and temporal relationships between the statistical moments of the waterbody size distributions underscore the dominance of topographic controls in lowland permafrost areas. These results provide motivation for further analyses of the factors involved in waterbody development and spatial distribution and for investigations into the possibility of using statistical moments to predict future hydrologic dynamics in the Arctic. KW - permafrost KW - hydrology KW - waterbodies KW - size distribution KW - thermokarst KW - statistical moments KW - ponds KW - lakes Y1 - 2019 U6 - https://doi.org/10.3389/feart.2019.00005 SN - 2296-6463 VL - 7 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Heinrichs, Steffi A1 - Ammer, Christian A1 - Mund, Martina A1 - Boch, Steffen A1 - Budde, Sabine A1 - Fischer, Markus A1 - Mueller, Joerg A1 - Schoening, Ingo A1 - Schulze, Ernst-Detlef A1 - Schmidt, Wolfgang A1 - Weckesser, Martin A1 - Schall, Peter T1 - Landscape-Scale Mixtures of Tree Species are More Effective than Stand-Scale Mixtures for Biodiversity of Vascular Plants, Bryophytes and Lichens JF - Forests N2 - Tree species diversity can positively affect the multifunctionality of forests. This is why conifer monocultures of Scots pine and Norway spruce, widely promoted in Central Europe since the 18th and 19th century, are currently converted into mixed stands with naturally dominant European beech. Biodiversity is expected to benefit from these mixtures compared to pure conifer stands due to increased abiotic and biotic resource heterogeneity. Evidence for this assumption is, however, largely lacking. Here, we investigated the diversity of vascular plants, bryophytes and lichens at the plot (alpha diversity) and at the landscape (gamma diversity) level in pure and mixed stands of European beech and conifer species (Scots pine, Norway spruce, Douglas fir) in four regions in Germany. We aimed to identify compositions of pure and mixed stands in a hypothetical forest landscape that can optimize gamma diversity of vascular plants, bryophytes and lichens within regions. Results show that gamma diversity of the investigated groups is highest when a landscape comprises different pure stands rather than tree species mixtures at the stand scale. Species mainly associated with conifers rely on light regimes that are only provided in pure conifer forests, whereas mixtures of beech and conifers are more similar to beech stands. Combining pure beech and pure conifer stands at the landscape scale can increase landscape level biodiversity and conserve species assemblages of both stand types, while landscapes solely composed of stand scale tree species mixtures could lead to a biodiversity reduction of a combination of investigated groups of 7 up to 20%. KW - Fagus sylvatica KW - Pinus sylvestris KW - Picea abies KW - Pseudotsuga menziesii KW - forest management KW - tree species diversity KW - forest conversion KW - gamma diversity KW - landscape scale KW - Biodiversity Exploratories Y1 - 2019 U6 - https://doi.org/10.3390/f10010073 SN - 1999-4907 VL - 10 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Al Nakeeb, Noah A1 - Kochovski, Zdravko A1 - Li, Tingting A1 - Zhang, Youjia A1 - Lu, Yan A1 - Schmidt, Bernhard V. K. J. T1 - Poly(ethylene glycol) brush-b-poly(N-vinylpyrrolidone)-based double hydrophilic block copolymer particles crosslinked via crystalline alpha-cyclodextrin domains JF - RSC Advances N2 - Self-assembly of block copolymers is a significant area of polymer science. The self-assembly of completely water-soluble block copolymers is of particular interest, albeit a challenging task. In the present work the self-assembly of a linear-brush architecture block copolymer, namely poly(N-vinylpyrrolidone)-b-poly(oligoethylene glycol methacrylate) (PVP-b-POEGMA), in water is studied. Moreover, the assembled structures are crosslinked via alpha-CD host/guest complexation in a supramolecular way. The crosslinking shifts the equilibrium toward aggregate formation without switching off the dynamic equilibrium of double hydrophilic block copolymer (DHBC). As a consequence, the self-assembly efficiency is improved without extinguishing the unique DHBC self-assembly behavior. In addition, decrosslinking could be induced without a change in concentration by adding a competing complexation agent for alpha-CD. The self-assembly behavior was followed by DLS measurement, while the presence of the particles could be observed via cryo-TEM before and after crosslinking. Y1 - 2019 U6 - https://doi.org/10.1039/c8ra10672j SN - 2046-2069 VL - 9 IS - 9 SP - 4993 EP - 5001 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Barbosa, Luis Romero A1 - de Lira, Nicholas Borges A1 - Rabelo Coelho, Victor Hugo A1 - Bernard Passerat de Silans, Alain Marie A1 - Gadelha, Andre Nobrega A1 - Almeida, Cristiano das Neves T1 - Stability of Soil Moisture Patterns Retrieved at Different Temporal Resolutions in a Tropical Watershed JF - Revista brasileira de ciencias do solo N2 - Above and underground hydrological processes depend on soil moisture (SM) variability, driven by different environmental factors that seldom are well-monitored, leading to a misunderstanding of soil water temporal patterns. This study investigated the stability of the SM temporal dynamics to different monitoring temporal resolutions around the border between two soil types in a tropical watershed. Four locations were instrumented in a small-scale watershed (5.84 km(2)) within the tropical coast of Northeast Brazil, encompassing different soil types (Espodossolo Humiluvico or Carbic Podzol, and Argissolo Vermelho-Amarelo or Haplic Acrisol), land covers (Atlantic Forest, bush vegetation, and grassland) and topographies (flat and moderate slope). The SM was monitored at a temporal resolution of one hour along the 2013-2014 hydrological year and then resampled a resolutions of 6 h, 12 h, 1 day, 2 days, 4 days, 7 days, and 15 days. Descriptive statistics, temporal variability, time-stability ranking, and hierarchical clustering revealed uneven associations among SM time components. The results show that the time-invariant component ruled SM temporal variability over the time-varying parcel, either at high or low temporal resolutions. Time-steps longer than 2 days affected the mean statistical metrics of the SM time-variant parcel. Additionally, SM at downstream and upstream sites behaved differently, suggesting that the temporal mean was regulated by steady soil properties (slope, restrictive layer, and soil texture), whereas their temporal anomalies were driven by climate (rainfall) and hydrogeological (groundwater level) factors. Therefore, it is concluded that around the border between tropical soil types, the distinct behaviour of time-variant and time-invariant components of SM time series reflects different combinations of their soil properties. KW - soil moisture variability KW - time-domain reflectometry KW - temporal resolution KW - Carbic Podzol KW - Haplic Acrisol Y1 - 2019 U6 - https://doi.org/10.1590/18069657rbcs20180236 SN - 0100-0683 VL - 43 PB - Sociedade Brasileira de Ciencia do Solo CY - Vicosa ER - TY - JOUR A1 - März, Moses Alexander T1 - Imagining a politics of relation BT - Glissant’s border thought and the German border JF - Tydskrif vir letterkunde N2 - This study explores the theoretical and political potentials of Édouard Glissant’s philosophy of relation and its approach to the issues of borders, migration, and the setup of political communities as proposed by his pensée nouvelle de la frontière (new border thought), against the background of the German migration crisis of 2015. The main argument of this article is that Glissant’s work offers an alternative epistemological and normative framework through which the contemporary political issues arising around the phenomenon of repressive border regimes can be studied. To demonstrate this point, this article works with Glissant’s border thought as an analytical lens and proposes a pathway for studying the contemporary German border regime. Particular emphasis is placed on the identification of potential areas where a Glissantian politics of relation could intervene with the goal of transforming borders from impermeable walls into points of passage. By exploring the political implications of his border thought, as well as the larger philosophical context from which it emerges, while using a transdisciplinary approach that borrows from literary and political studies, this work contributes to ongoing debates in postcolonial studies on borders and borderlessness, as well as Glissant’s political legacy in the twenty-first century. KW - Edouard Glissant KW - politics of relation KW - Germany KW - border regime Y1 - 2019 U6 - https://doi.org/10.17159/2309-9070/tvl.v.56i1.6271 SN - 0041-476X VL - 56 IS - 1 SP - 49 EP - 61 PB - University of Pretoria CY - Pretoria ER - TY - JOUR A1 - Steppa, Constantin Beverly A1 - Holch, Tim Lukas T1 - HexagDLy-Processing hexagonally sampled data with CNNs in PyTorch JF - SoftwareX N2 - HexagDLy is a Python-library extending the PyTorch deep learning framework with convolution and pooling operations on hexagonal grids. It aims to ease the access to convolutional neural networks for applications that rely on hexagonally sampled data as, for example, commonly found in ground-based astroparticle physics experiments. KW - Convolutional neural networks KW - Hexagonal grid KW - PyTorch KW - Astroparticle physics Y1 - 2019 U6 - https://doi.org/10.1016/j.softx.2019.02.010 SN - 2352-7110 VL - 9 SP - 193 EP - 198 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Crane, Kylie Ann T1 - Anthropocene Presences and the Limits of Deferral BT - Alexis Wright's Carpentaria and The Swan Book JF - Open library of humanities N2 - Literary criticism, particularly ecocriticism, occupies an uneasy position with regard to activism: reading books (or plays, or poems) seems like a rather leisurely activity to be undertaking if our environment—our planet—is in crisis. And yet, critiquing the narratives that structure worlds and discourses is key to the activities of the (literary) critic in this time of crisis. If this crisis manifests as a ‘crisis of imagination’ (e.g. Ghosh), I argue that this not so much a crisis of the absence of texts that address the environmental disaster, but rather a failure to comprehend the presences of the Anthropocene in the present. To interpret (literary) texts in this framework must entail acknowledging and scrutinising the extent of the incapacity of the privileged reader to comprehend the crisis as presence and present rather than spatially or temporally remote. The readings of the novels Carpentaria (2006) and The Swan Book (2013) by Waanyi writer Alexis Wright (Australia) trace the uneven presences of Anthropocenes in the present by way of bringing future worlds (The Swan Book) to the contemporary (Carpentaria). In both novels, protagonists must forge survival amongst ruins of the present and future: the depicted worlds, in particular the representations of the disenfranchisement of indigenous inhabitants of the far north of the Australian continent, emerge as a critique of the intersections of capitalist and colonial projects that define modernity and its impact on the global climate. Y1 - 2019 U6 - https://doi.org/10.16995/olh.348 SN - 2056-6700 VL - 5 IS - 1 PB - Open library of humanities CY - Cambridge ER - TY - JOUR A1 - Petitgirard, Sylvian A1 - Sahle, C. J. A1 - Weis, C. A1 - Gilmore, K. A1 - Spiekermann, Georg A1 - Tse, J. S. A1 - Wilke, Max A1 - Cavallari, C. A1 - Cerantola, V A1 - Sternemann, Christian T1 - Magma properties at deep Earth’s conditions from electronic structure of silica JF - Geochemical perspectives letters N2 - SiO(2 )is the main component of silicate melts and thus controls their network structure and physical properties. The compressibility and viscosities of melts at depth are governed by their short range atomic and electronic structure. We measured the O K-edge and the Si L-2,L-3-edge in silica up to 110 GPa using X-ray Raman scattering spectroscopy, and found a striking match to calculated spectra based on structures from molecular dynamic simulations. Between 20 and 27 GPa, Si-[4] species are converted into a mixture of Si-[5] and Si-[6] species and between 60 and 70 GPa, Si-[6] becomes dominant at the expense of Si-[5] with no further increase up to at least 110 GPa. Coordination higher than 6 is only reached beyond 140 GPa, corroborating results from Brillouin scattering. Network modifying elements in silicate melts may shift this change in coordination to lower pressures and thus magmas could be denser than residual solids at the depth of the core-mantle boundary. Y1 - 2019 U6 - https://doi.org/10.7185/geochemlet.1902 SN - 2410-339X SN - 2410-3403 VL - 9 SP - 32 EP - 37 PB - Association of Geochemistry CY - Paris ER - TY - JOUR A1 - Distelmeyer, Jan T1 - From object to process BT - Interface politics of networked computerization JF - Artnodes N2 - One of the most difficult tasks today is trying to grasp the presence of computing. The almost ubiquitous and diverse forms of networked computers (in all their stationary, mobile, embedded, and autonomous modes) create a nearly overwhelming complexity. To speak of what is here evading and present at the same time, the paper proposes to reconsider the concept of interface, its historical roots, and its heuristic advantages for an analysis and critique of the current and especially everyday spread of computerization. The question of interfaces leads to isolable conditions and processes of conduction, as well as to the complexity of the cooperation formed by them. It opens both an investigative horizon and a mode of analysis, which always asks for further interface levels involved in the phenomenon I am currently investigating. As an example, the paper turns to the displacement of the file with the launch of the iPhone in 2007 and its comeback in 2017 with the "Files" apps. Both developments are profoundly related to the establishment of computers as permanently networked machines, whereby their functionality, depresentations, and ideology come into focus. KW - interface KW - conduction KW - network KW - ideology KW - depresentation KW - digital objects Y1 - 2019 U6 - https://doi.org/10.7238/a.v0i24.3300 SN - 1695-5951 IS - 24 SP - 83 EP - 90 PB - Universitat Oberta de Catalunya CY - Barcelona ER - TY - JOUR A1 - Mota, Cristiano A1 - Esmaeeli Moghaddam Tabalvandani, Mariam A1 - Coelho, Catarina A1 - Santos-Silva, Teresa A1 - Wolff, Martin A1 - Foti, Alessandro A1 - Leimkühler, Silke A1 - Romao, Maria Joao T1 - Human aldehyde oxidase (hAOX1) BT - structure determination of the Moco-free form of the natural variant G1269R and biophysical studies of single nucleotide polymorphisms JF - FEBS Open Bio N2 - Human aldehyde oxidase (hAOX1) is a molybdenum enzyme with high toxicological importance, but its physiological role is still unknown. hAOX1 metabolizes different classes of xenobiotics and is one of the main drug-metabolizing enzymes in the liver, along with cytochrome P450. hAOX1 oxidizes and inactivates a large number of drug molecules and has been responsible for the failure of several phase I clinical trials. The interindividual variability of drug-metabolizing enzymes caused by single nucleotide polymorphisms (SNPs) is highly relevant in pharmaceutical treatments. In this study, we present the crystal structure of the inactive variant G1269R, revealing the first structure of a molybdenum cofactor (Moco)-free form of hAOX1. These data allowed to model, for the first time, the flexible Gate 1 that controls access to the active site. Furthermore, we inspected the thermostability of wild-type hAOX1 and hAOX1 with various SNPs (L438V, R1231H, G1269R or S1271L) by CD spectroscopy and ThermoFAD, revealing that amino acid exchanges close to the Moco site can impact protein stability up to 10 degrees C. These results correlated with biochemical and structural data and enhance our understanding of hAOX1 and the effect of SNPs in the gene encoding this enzyme in the human population. EnzymesAldehyde oxidase (); xanthine dehydrogenase (); xanthine oxidase (). DatabasesStructural data are available in the Protein Data Bank under the accession number . KW - human aldehyde oxidase KW - molybdenum cofactor KW - single nucleotide polymorphism KW - xanthine oxidase Y1 - 2019 U6 - https://doi.org/10.1002/2211-5463.12617 SN - 2211-5463 VL - 9 IS - 5 SP - 925 EP - 934 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Zorn, Edgar Ulrich A1 - Le Corvec, Nicolas A1 - Varley, Nick R. A1 - Salzer, Jacqueline T. A1 - Walter, Thomas R. A1 - Navarro-Ochoa, Carlos A1 - Vargas-Bracamontes, Dulce M. A1 - Thiele, Samuel T. A1 - Arámbula Mendoza, Raúl T1 - Load stress controls on directional lava dome growth at Volcan de Colima, Mexico JF - Frontiers in Earth Science N2 - During eruptive activity of andesitic stratovolcanoes, the extrusion of lava domes, their collapse and intermittent explosions are common volcanic hazards. Many lava domes grow in a preferred direction, in turn affecting the direction of lava flows and pyroclastic density currents. Access to active lava domes is difficult and hazardous, so detailed data characterizing lava dome growth are typically limited, keeping the processes controlling the directionality of extrusions unclear. Here we combine TerraSAR-X satellite radar observations with high-resolution airborne photogrammetry to assess morphological changes, and perform finite element modeling to investigate the impact of loading stress on shallow magma ascent directions associated with lava dome extrusion and crater formation at Volcan de Colima, Mexico. The TerraSAR-X data, acquired in similar to 1-m resolution spotlight mode, enable us to derive a chronology of the eruptive processes from intensity-based time-lapse observations of the general crater and dome evolution. The satellite images are complemented by close-range airborne photos, processed by the Structure-from-Motion workflow. This allows the derivation of high-resolution digital elevation models, providing insight into detailed loading and unloading features. During the observation period from Jan-2013 to Feb-2016, we identify a dominantly W-directed dome growth and lava flow production until Jan-2015. In Feb-2015, following the removal of the active summit dome, the surface crater widened and elongated along a NE-SW axis. Later in May-2015, a new dome grew toward the SW of the crater while a separate vent developed in the NE of the crater, reflecting a change in the direction of magma ascent and possible conduit bifurcation. Finite element models show a significant stress change in agreement with the observed magma ascent direction changes in response to the changing surface loads, both for loading (dome growth) and unloading (crater forming excavation) cases. These results allow insight into shallow dome growth dynamics and the migration of magma ascent in response to changing volcano summit morphology. They further highlight the importance of detailed volcano summit morphology surveillance, as changes in direction or location of dome extrusion may have major implications regarding the directions of potential volcanic hazards, such as pyroclastic density currents generated by dome collapse. KW - lava dome KW - load stress KW - Volcan de Colima KW - TerraSAR-X KW - photogrammetry KW - finite element modeling Y1 - 2019 U6 - https://doi.org/10.3389/feart.2019.00084 SN - 2296-6463 VL - 7 PB - Frontiers Media CY - Lausanne ER - TY - JOUR A1 - Wyckmans, Florent A1 - Otto, A. Ross A1 - Sebold, Miriam Hannah A1 - Daw, Nathaniel A1 - Bechara, Antoine A1 - Saeremans, Mélanie A1 - Kornreich, Charles A1 - Chatard, Armand A1 - Jaafari, Nemat A1 - Noël, Xavier T1 - Reduced model-based decision-making in gambling disorder JF - Scientific reports N2 - Compulsive behaviors (e.g., addiction) can be viewed as an aberrant decision process where inflexible reactions automatically evoked by stimuli (habit) take control over decision making to the detriment of a more flexible (goal-oriented) behavioral learning system. These behaviors are thought to arise from learning algorithms known as "model-based" and "model-free" reinforcement learning. Gambling disorder, a form of addiction without the confound of neurotoxic effects of drugs, showed impaired goal-directed control but the way in which problem gamblers (PG) orchestrate model-based and model-free strategies has not been evaluated. Forty-nine PG and 33 healthy participants (CP) completed a two-step sequential choice task for which model-based and model-free learning have distinct and identifiable trial-by-trial learning signatures. The influence of common psychopathological comorbidities on those two forms of learning were investigated. PG showed impaired model-based learning, particularly after unrewarded outcomes. In addition, PG exhibited faster reaction times than CP following unrewarded decisions. Troubled mood, higher impulsivity (i.e., positive and negative urgency) and current and chronic stress reported via questionnaires did not account for those results. These findings demonstrate specific reinforcement learning and decision-making deficits in behavioral addiction that advances our understanding and may be important dimensions for designing effective interventions. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-56161-z SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Nüsken, Nikolas A1 - Reich, Sebastian A1 - Rozdeba, Paul J. T1 - State and parameter estimation from observed signal increments JF - Entropy : an international and interdisciplinary journal of entropy and information studies N2 - The success of the ensemble Kalman filter has triggered a strong interest in expanding its scope beyond classical state estimation problems. In this paper, we focus on continuous-time data assimilation where the model and measurement errors are correlated and both states and parameters need to be identified. Such scenarios arise from noisy and partial observations of Lagrangian particles which move under a stochastic velocity field involving unknown parameters. We take an appropriate class of McKean-Vlasov equations as the starting point to derive ensemble Kalman-Bucy filter algorithms for combined state and parameter estimation. We demonstrate their performance through a series of increasingly complex multi-scale model systems. KW - parameter estimation KW - continuous-time data assimilation KW - ensemble Kalman filter KW - correlated noise KW - multi-scale diffusion processes Y1 - 2019 U6 - https://doi.org/10.3390/e21050505 SN - 1099-4300 VL - 21 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Müller, Katharina A1 - Foerstendorf, Harald A1 - Steudtner, Robin A1 - Tsushima, Satoru A1 - Kumke, Michael Uwe A1 - Lefèvre, Grégory A1 - Rothe, Jörg A1 - Mason, Harris A1 - Szabó, Zoltán A1 - Yang, Ping A1 - Adam, Christian K. R. A1 - André, Rémi A1 - Brennenstuhl, Katlen A1 - Chiorescu, Ion A1 - Cho, Herman M. A1 - Creff, Gaëlle A1 - Coppin, Frédéric A1 - Dardenne, Kathy A1 - Den Auwer, Christophe A1 - Drobot, Björn A1 - Eidner, Sascha A1 - Hess, Nancy J. A1 - Kaden, Peter A1 - Kremleva, Alena A1 - Kretzschmar, Jerome A1 - Krüger, Sven A1 - Platts, James A. A1 - Panak, Petra A1 - Polly, Robert A1 - Powell, Brian A. A1 - Rabung, Thomas A1 - Redon, Roland A1 - Reiller, Pascal E. A1 - Rösch, Notker A1 - Rossberg, André A1 - Scheinost, Andreas C. A1 - Schimmelpfennig, Bernd A1 - Schreckenbach, Georg A1 - Skerencak-Frech, Andrej A1 - Sladkov, Vladimir A1 - Solari, Pier Lorenzo A1 - Wang, Zheming A1 - Washton, Nancy M. A1 - Zhang, Xiaobin T1 - Interdisciplinary Round-Robin Test on molecular spectroscopy of the U(VI) Acetate System JF - ACS omega / American Chemical Society N2 - A comprehensive molecular analysis of a simple aqueous complexing system. U(VI) acetate. selected to be independently investigated by various spectroscopic (vibrational, luminescence, X-ray absorption, and nuclear magnetic resonance spectroscopy) and quantum chemical methods was achieved by an international round-robin test (RRT). Twenty laboratories from six different countries with a focus on actinide or geochemical research participated and contributed to this scientific endeavor. The outcomes of this RRT were considered on two levels of complexity: first, within each technical discipline, conformities as well as discrepancies of the results and their sources were evaluated. The raw data from the different experimental approaches were found to be generally consistent. In particular, for complex setups such as accelerator-based X-ray absorption spectroscopy, the agreement between the raw data was high. By contrast, luminescence spectroscopic data turned out to be strongly related to the chosen acquisition parameters. Second, the potentials and limitations of coupling various spectroscopic and theoretical approaches for the comprehensive study of actinide molecular complexes were assessed. Previous spectroscopic data from the literature were revised and the benchmark data on the U(VI) acetate system provided an unambiguous molecular interpretation based on the correlation of spectroscopic and theoretical results. The multimethodologic approach and the conclusions drawn address not only important aspects of actinide spectroscopy but particularly general aspects of modern molecular analytical chemistry. Y1 - 2019 U6 - https://doi.org/10.1021/acsomega.9b00164 SN - 2470-1343 VL - 4 IS - 5 SP - 8167 EP - 8177 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Asgarimehr, Milad A1 - Wickert, Jens A1 - Reich, Sebastian T1 - Evaluating impact of rain attenuation on space-borne GNSS reflectometry wind speeds JF - Remote Sensing N2 - The novel space-borne Global Navigation Satellite System Reflectometry (GNSS-R) technique has recently shown promise in monitoring the ocean state and surface wind speed with high spatial coverage and unprecedented sampling rate. The L-band signals of GNSS are structurally able to provide a higher quality of observations from areas covered by dense clouds and under intense precipitation, compared to those signals at higher frequencies from conventional ocean scatterometers. As a result, studying the inner core of cyclones and improvement of severe weather forecasting and cyclone tracking have turned into the main objectives of GNSS-R satellite missions such as Cyclone Global Navigation Satellite System (CYGNSS). Nevertheless, the rain attenuation impact on GNSS-R wind speed products is not yet well documented. Evaluating the rain attenuation effects on this technique is significant since a small change in the GNSS-R can potentially cause a considerable bias in the resultant wind products at intense wind speeds. Based on both empirical evidence and theory, wind speed is inversely proportional to derived bistatic radar cross section with a natural logarithmic relation, which introduces high condition numbers (similar to ill-posed conditions) at the inversions to high wind speeds. This paper presents an evaluation of the rain signal attenuation impact on the bistatic radar cross section and the derived wind speed. This study is conducted simulating GNSS-R delay-Doppler maps at different rain rates and reflection geometries, considering that an empirical data analysis at extreme wind intensities and rain rates is impossible due to the insufficient number of observations from these severe conditions. Finally, the study demonstrates that at a wind speed of 30 m/s and incidence angle of 30 degrees, rain at rates of 10, 15, and 20 mm/h might cause overestimation as large as approximate to 0.65 m/s (2%), 1.00 m/s (3%), and 1.3 m/s (4%), respectively, which are still smaller than the CYGNSS required uncertainty threshold. The simulations are conducted in a pessimistic condition (severe continuous rainfall below the freezing height and over the entire glistening zone) and the bias is expected to be smaller in size in real environments. KW - GNSS Reflectometry KW - wind speed KW - rain effect KW - rain attenuation KW - DDM simulation Y1 - 2019 U6 - https://doi.org/10.3390/rs11091048 SN - 2072-4292 VL - 11 IS - 9 PB - MDPI CY - Basel ER - TY - JOUR A1 - Park, Jungsu A1 - Batalla, Ramon J. A1 - Birgand, Francois A1 - Esteves, Michel A1 - Gentile, Francesco A1 - Harrington, Joseph R. A1 - Navratil, Oldrich A1 - Lopez-Tarazon, José Andrés A1 - Vericat, Damia T1 - Influences of Catchment and River Channel Characteristics on the Magnitude and Dynamics of Storage and Re-Suspension of Fine Sediments in River Beds JF - Water N2 - Fine particles or sediments are one of the important variables that should be considered for the proper management of water quality and aquatic ecosystems. In the present study, the effect of catchment characteristics on the performance of an already developed model for the estimation of fine sediments dynamics between the water column and sediment bed was tested, using 13 catchments distributed worldwide. The model was calibrated to determine two optimal model parameters. The first is the filtration parameter, which represents the filtration of fine sediments through pores of the stream bed during the recession period of a flood event. The second parameter is the bed erosion parameter that represents the active layer, directly related to the re-suspension of fine sediments during a flood event. A dependency of the filtration parameter with the catchment area was observed in catchments smaller than 100 km(2), whereas no particular relationship was observed for larger catchments (>100 km(2)). In contrast, the bed erosion parameter does not show a noticeable dependency with the area or other environmental characteristics. The model estimated the mass of fine sediments released from the sediment bed to the water column during flood events in the 13 catchments within 23% bias. KW - bed erosion KW - catchment area KW - filtration KW - sediment accumulation KW - sediment bed fluidization KW - sediment re-suspension Y1 - 2019 U6 - https://doi.org/10.3390/w11050878 SN - 2073-4441 VL - 11 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Menzel, Ralf A1 - Heuer, Axel A1 - Milonni, Peter W. T1 - Entanglement, Complementarity, and Vacuum Fields in Spontaneous Parametric Down-Conversion JF - Atoms N2 - Using two crystals for spontaneous parametric down-conversion in a parallel setup, we observe two-photon interference with high visibility. The high visibility is consistent with complementarity and the absence of which-path information. The observations are explained as the effects of entanglement or equivalently in terms of interfering probability amplitudes and also by the calculation of a second-order field correlation function in the Heisenberg picture. The latter approach brings out explicitly the role of the vacuum fields in the down-conversion at the crystals and in the photon coincidence counting. For comparison, we show that the Hong-Ou-Mandel dip can be explained by the same approach in which the role of the vacuum signal and idler fields, as opposed to entanglement involving vacuum states, is emphasized. We discuss the fundamental limitations of a theory in which these vacuum fields are treated as classical, stochastic fields. KW - complementarity KW - vacuum fields KW - entanglement KW - Hong-Ou-Mandel effect KW - spontaneous parametric down-conversion Y1 - 2019 U6 - https://doi.org/10.3390/atoms7010027 SN - 2218-2004 VL - 7 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Sroka, Pavel A1 - Godunko, Roman J. A1 - Rutschmann, Sereina A1 - Angeli, Kamila B. A1 - Salles, Frederico F. A1 - Gattolliat, Jean-Luc T1 - A new species of Bungona in Turkey (Ephemeroptera, Baetidae) BT - an unexpected biogeographic pattern within a pantropical complex of mayflies JF - Zoosytematics and evolution N2 - By using an integrative approach, we describe a new species of mayfly, Bungona (Chopralla) pontica sp. n., from Turkey. The discovery of a representative of the tropical mayfly genus Bungona in the Middle East is rather unexpected. The new species shows all the main morphological characters of the subgenus Chopralla, which has its closest related species occurring in southeastern Asia. Barcoding clearly indicated that the new species represents an independent lineage isolated for a very long time from other members of the complex. The claw is equipped with two rows of three or four flattened denticles. This condition is a unique feature of Bungona (Chopralla) pontica sp. n. among West Palaearctic mayfly species. Within the subgenus Chopralla, the species can be identified by the presence of a simple, not bifid right prostheca (also present only in Bungona (Chopralla) liebenauae (Soldan, Braasch & Muu, 1987)), the shape of the labial palp, and the absence of protuberances on pronotum. KW - Biogeography KW - Cloeodes complex KW - Chopralla KW - integrative taxonomy KW - Middle East KW - new species Y1 - 2019 U6 - https://doi.org/10.3897/zse.95.29487 SN - 1860-0743 VL - 95 IS - 1 SP - 1 EP - 13 PB - Pensoft Publ. CY - Sofia ER - TY - JOUR A1 - Essl, Franz A1 - Dawson, Wayne A1 - Kreft, Holger A1 - Pergl, Jan A1 - Pysek, Petr A1 - van Kleunen, Mark A1 - Weigelt, Patrick A1 - Mang, Thomas A1 - Dullinger, Stefan A1 - Lenzner, Bernd A1 - Moser, Dietmar A1 - Maurel, Noelie A1 - Seebens, Hanno A1 - Stein, Anke A1 - Weber, Ewald A1 - Chatelain, Cyrille A1 - Inderjit, A1 - Genovesi, Piero A1 - Kartesz, John A1 - Morozova, Olga A1 - Nishino, Misako A1 - Nowak, Pauline M. A1 - Pagad, Shyama A1 - Shu, Wen-sheng A1 - Winter, Marten T1 - Drivers of the relative richness of naturalized and invasive plant species on Earth JF - AoB PLANTS N2 - Biological invasions are a defining feature of the Anthropocene, but the factors that determine the spatially uneven distribution of alien plant species are still poorly understood. Here, we present the first global analysis of the effects of biogeographic factors, the physical environment and socio-economy on the richness of naturalized and invasive alien plants. We used generalized linear mixed-effects models and variation partitioning to disentangle the relative importance of individual factors, and, more broadly, of biogeography, physical environment and socio-economy. As measures of the magnitude of permanent anthropogenic additions to the regional species pool and of species with negative environmental impacts, we calculated the relative richness of naturalized (= RRN) and invasive (= RRI) alien plant species numbers adjusted for the number of native species in 838 terrestrial regions. Socio-economic factors (per-capita gross domestic product (GDP), population density, proportion of agricultural land) were more important in explaining RRI (similar to 50 % of the explained variation) than RRN (similar to 40 %). Warm-temperate and (sub)tropical regions have higher RRN than tropical or cooler regions. We found that socio-economic pressures are more relevant for invasive than for naturalized species richness. The expectation that the southern hemisphere is more invaded than the northern hemisphere was confirmed only for RRN on islands, but not for mainland regions nor for RRI. On average, islands have similar to 6-fold RRN, and >3-fold RRI compared to mainland regions. Eighty-two islands (=26 % of all islands) harbour more naturalized alien than native plants. Our findings challenge the widely held expectation that socio-economic pressures are more relevant for plant naturalization than for invasive plants. To meet international biodiversity targets and halt the detrimental consequences of plant invasions, it is essential to disrupt the connection between socio-economic development and plant invasions by improving pathway management, early detection and rapid response. KW - Alien species richness KW - biogeography KW - invasion stages KW - islands KW - pressures KW - vascular plants Y1 - 2019 U6 - https://doi.org/10.1093/aobpla/plz051 SN - 2041-2851 VL - 11 IS - 5 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - López de Guereñu, Anna A1 - Bastian, Philipp A1 - Wessig, Pablo A1 - John, Leonard A1 - Kumke, Michael Uwe T1 - Energy Transfer between Tm-Doped Upconverting Nanoparticles and a Small Organic Dye with Large Stokes Shift JF - Biosensors : open access journal N2 - Lanthanide-doped upconverting nanoparticles (UCNP) are being extensively studied for bioapplications due to their unique photoluminescence properties and low toxicity. Interest in RET applications involving UCNP is also increasing, but due to factors such as large sizes, ion emission distributions within the particles, and complicated energy transfer processes within the UCNP, there are still many questions to be answered. In this study, four types of core and core-shell NaYF4-based UCNP co-doped with Yb3+ and Tm3+ as sensitizer and activator, respectively, were investigated as donors for the Methyl 5-(8-decanoylbenzo[1,2-d:4,5-d ']bis([1,3]dioxole)-4-yl)-5-oxopentanoate (DBD-6) dye. The possibility of resonance energy transfer (RET) between UCNP and the DBD-6 attached to their surface was demonstrated based on the comparison of luminescence intensities, band ratios, and decay kinetics. The architecture of UCNP influenced both the luminescence properties and the energy transfer to the dye: UCNP with an inert shell were the brightest, but their RET efficiency was the lowest (17%). Nanoparticles with Tm3+ only in the shell have revealed the highest RET efficiencies (up to 51%) despite the compromised luminescence due to surface quenching. KW - resonance energy transfer KW - DBD dye KW - core shell UCNP KW - time-resolved luminescence Y1 - 2019 U6 - https://doi.org/10.3390/bios9010009 SN - 2079-6374 VL - 9 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Lartey, Nathaniel A1 - Tsiwah, Frank A1 - Amponsah, Clement A1 - Martinez-Ferreiro, Silvia A1 - Bastiaanse, Roelien T1 - Resumption in the production of focused constructions in Akan speakers with agrammatism JF - Aphasiology N2 - Background: The distribution of pronouns varies cross-linguistically. This distribution has led to conflicting results in studies that investigated pronoun resolution in agrammatic indviduals. In the investigation of pronominal resolution, the linguistic phenomenon of "resumption" is understudied in agrammatism. The construction of pronominal resolution in Akan presents the opportunity to thoroughly examine resumption. Aims: To start, the present study examines the production of (pronominal) resumption in Akan focus constructions (who-questions and focused declaratives). Second, we explore the effect of grammatical tone on the processing of pronominal (resumption) since Akan is a tonal language. Methods & Procedures: First, we tested the ability to distinguish linguistic and non-linguistic tone in Akan agrammatic speakers. Then, we administered an elicitation task to five Akan agrammatic individuals, controlling for the structural variations in the realization of resumption: focused who-questions and declaratives with (i) only a resumptive pronoun, (ii) only a clause determiner, (iii) a resumptive pronoun and a clause determiner co-occurring, and (iv) neither a resumptive pronoun nor a clause determiner. Outcomes & Results: Tone discrimination .both for pitch and for lexical tone was unimpaired. The production task demonstrated that the production of resumptive pronouns and clause determiners was intact. However, the production of declarative sentences in derived word order was impaired; wh-object questions were relatively well-preserved. Conclusions: We argue that the problems with sentence production are highly selective: linguistic tones and resumption are intact but word order is impaired in non-canonical declarative sentences. KW - Agrammatism KW - focus constructions KW - (pronominal) resumption KW - clause determiner KW - Akan Y1 - 2019 U6 - https://doi.org/10.1080/02687038.2019.1686746 VL - 34 IS - 3 SP - 343 EP - 364 PB - Routledge CY - London ER - TY - JOUR A1 - Nakaten, Natalie Christine A1 - Kempka, Thomas T1 - Techno-Economic Comparison of Onshore and Offshore Underground Coal Gasification End-Product Competitiveness JF - Energies N2 - Underground coal gasification (UCG) enables utilization of coal reserves, currently not economically exploitable due to complex geological boundary conditions. Hereby, UCG produces a high-calorific synthesis gas that can be used for generation of electricity, fuels, and chemical feedstock. The present study aims to identify economically-competitive, site-specific end-use options for onshore- and offshore-produced UCG synthesis gas, taking into account the capture and storage (CCS) and/or utilization (CCU) of produced CO2. Modeling results show that boundary conditions favoring electricity, methanol, and ammonia production expose low costs for air separation, low compression power requirements, and appropriate shares of H-2/N-2. Hereby, a gasification agent ratio of more than 30% oxygen by volume is not favorable from the economic and CO2 mitigation viewpoints. Compared to the costs of an offshore platform with its technical equipment, offshore drilling costs are marginal. Thus, uncertainties related to parameters influenced by drilling costs are negligible. In summary, techno-economic process modeling results reveal that air-blown gasification scenarios are the most cost-effective ones, while offshore UCG-CCS/CCU scenarios are up to 1.7 times more expensive than the related onshore processes. Hereby, all investigated onshore scenarios except from ammonia production under the assumed worst-case conditions are competitive on the European market. KW - underground coal gasification (UCG) KW - economics KW - cost of electricity (COE) KW - techno-economic model KW - methanol KW - ammonia KW - carbon capture and storage (CCS) KW - carbon capture and utilization (CCU) KW - electricity generation KW - process simulation Y1 - 2019 U6 - https://doi.org/10.3390/en12173252 SN - 1996-1073 VL - 12 IS - 17 PB - MDPI CY - Basel ER - TY - JOUR A1 - Lachmann, Sabrina C. A1 - Mettler-Altmann, Tabea A1 - Wacker, Alexander A1 - Spijkerman, Elly T1 - Nitrate or ammonium BT - Influences of nitrogen source on the physiology of a green alga JF - Ecology and evolution N2 - In freshwaters, algal species are exposed to different inorganic nitrogen (Ni) sources whose incorporation varies in biochemical energy demand. We hypothesized that due to the lesser energy requirement of ammonium (NH4+)-use, in contrast to nitrate (NO3-)-use, more energy remains for other metabolic processes, especially under CO2-and phosphorus (Pi) limiting conditions. Therefore, we tested differences in cell characteristics of the green alga Chlamydomonas acidophila grown on NH4+ or NO3- under covariation of CO2 and Pi-supply in order to determine limitations, in a full-factorial design. As expected, results revealed higher carbon fixation rates for NH4+ grown cells compared to growth with NO3- under low CO2 conditions. NO3- -grown cells accumulated more of the nine analyzed amino acids, especially under Pi-limited conditions, compared to cells provided with NH4+. This is probably due to a slower protein synthesis in cells provided with NO3-. In contrast to our expectations, compared to NH4+ -grown cells NO3- -grown cells had higher photosynthetic efficiency under Pi-limitation. In conclusion, growth on the Ni-source NH4+ did not result in a clearly enhanced Ci-assimilation, as it was highly dependent on Pi and CO2 conditions (replete or limited). Results are potentially connected to the fact that C. acidophila is able to use only CO2 as its inorganic carbon (Ci) source. KW - amino acids KW - carbon uptake kinetics KW - CO2 conditions KW - nitrogen KW - phosphorus limitation Y1 - 2019 U6 - https://doi.org/10.1002/ece3.4790 SN - 2045-7758 VL - 9 IS - 3 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Grimm-Seyfarth, Annegret A1 - Mihoub, Jean-Baptiste A1 - Henle, Klaus T1 - Functional traits determine the different effects of prey, predators, and climatic extremes on desert reptiles JF - Ecosphere : the magazine of the International Ecology University N2 - Terrestrial reptiles are particularly vulnerable to climate change. Their highest density and diversity can be found in hot drylands, ecosystems which demonstrate extreme climatic conditions. However, reptiles are not isolated systems but part of a large species assemblage with many trophic dependencies. While direct relations among climatic conditions, invertebrates, vegetation, or reptiles have already been explored, to our knowledge, species’ responses to direct and indirect pathways of multiple climatic and biotic factors and their interactions have rarely been examined comprehensively. We investigated direct and indirect effects of climatic and biotic parameters on the individual (body condition) and population level (occupancy) of eight abundant lizard species with different functional traits in an arid Australian lizard community using a 30‐yr multi‐trophic monitoring study. We used structural equation modeling to disentangle single and interactive effects. We then assessed whether species could be grouped into functional groups according to their functional traits and their responses to different parameters. We found that lizard species differed strongly in how they responded to climatic and biotic factors. However, the factors to which they responded seemed to be determined by their functional traits. While responses on body condition were determined by habitat, activity time, and prey, responses on occupancy were determined by habitat specialization, body size, and longevity. Our findings highlight the importance of indirect pathways through climatic and biotic interactions, which should be included into predictive models to increase accuracy when predicting species’ responses to climate change. Since one might never obtain all mechanistic pathways at the species level, we propose an approach of identifying relevant species traits that help grouping species into functional groups at different ecological levels, which could then be used for predictive modeling. KW - Australia KW - climate change KW - Gekkonidae KW - periodic flooding KW - Scincidae KW - species functional traits KW - species interactions KW - structural equation modeling Y1 - 2019 U6 - https://doi.org/10.1002/ecs2.2865 SN - 2150-8925 VL - 10 IS - 9 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Kagel, Heike A1 - Bier, Frank Fabian A1 - Frohme, Marcus A1 - Glökler, Jörn F. T1 - A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids JF - Scientific reports N2 - Most biochemical reactions depend on the pH value of the aqueous environment and some are strongly favoured to occur in an acidic environment. A non-invasive control of pH to tightly regulate such reactions with defined start and end points is a highly desirable feature in certain applications, but has proven difficult to achieve so far. We report a novel optical approach to reversibly control a typical biochemical reaction by changing the pH and using acid phosphatase as a model enzyme. The reversible photoacid G-acid functions as a proton donor, changing the pH rapidly and reversibly by using high power UV LEDs as an illumination source in our experimental setup. The reaction can be tightly controlled by simply switching the light on and off and should be applicable to a wide range of other enzymatic reactions, thus enabling miniaturization and parallelization through non-invasive optical means. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-50867-w SN - 2045-2322 VL - 9 PB - Nature Publishing Group CY - London ER - TY - JOUR A1 - Chapman, Eric M. A1 - Lant, Benjamin A1 - Ohashi, Yota A1 - Yu, Bin A1 - Schertzberg, Michael A1 - Go, Christopher A1 - Dogra, Deepika A1 - Koskimaki, Janne A1 - Girard, Romuald A1 - Li, Yan A1 - Fraser, Andrew G. A1 - Awad, Issam A. A1 - Abdelilah-Seyfried, Salim A1 - Gingras, Anne-Claude A1 - Derry, William Brent T1 - A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis JF - Nature Communications N2 - Apoptotic death of cells damaged by genotoxic stress requires regulatory input from surrounding tissues. The C. elegans scaffold protein KRI-1, ortholog of mammalian KRIT1/CCM1, permits DNA damage-induced apoptosis of cells in the germline by an unknown cell non-autonomous mechanism. We reveal that KRI-1 exists in a complex with CCM-2 in the intestine to negatively regulate the ERK-5/MAPK pathway. This allows the KLF-3 transcription factor to facilitate expression of the SLC39 zinc transporter gene zipt-2.3, which functions to sequester zinc in the intestine. Ablation of KRI-1 results in reduced zinc sequestration in the intestine, inhibition of IR-induced MPK-1/ERK1 activation, and apoptosis in the germline. Zinc localization is also perturbed in the vasculature of krit1(-/-) zebrafish, and SLC39 zinc transporters are mis-expressed in Cerebral Cavernous Malformations (CCM) patient tissues. This study provides new insights into the regulation of apoptosis by cross-tissue communication, and suggests a link between zinc localization and CCM disease. Y1 - 2019 U6 - https://doi.org/10.1038/s41467-019-09829-z SN - 2041-1723 VL - 10 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Demal, Till Joscha A1 - Heise, Melina A1 - Reiz, Benedikt A1 - Dogra, Deepika A1 - Braenne, Ingrid A1 - Reichenspurner, Hermann A1 - Männer, Jörg A1 - Aherrahrou, Zouhair A1 - Schunkert, Heribert A1 - Erdmann, Jeanette A1 - Abdelilah-Seyfried, Salim T1 - A familial congenital heart disease with a possible multigenic origin involving a mutation in BMPR1A JF - Scientific reports N2 - The genetics of many congenital heart diseases (CHDs) can only unsatisfactorily be explained by known chromosomal or Mendelian syndromes. Here, we present sequencing data of a family with a potentially multigenic origin of CHD. Twelve of nineteen family members carry a familial mutation [NM_004329.2:c.1328 G > A (p.R443H)] which encodes a predicted deleterious variant of BMPR1A. This mutation co-segregates with a linkage region on chromosome 1 that associates with the emergence of severe CHDs including Ebstein’s anomaly, atrioventricular septal defect, and others. We show that the continuous overexpression of the zebrafish homologous mutation bmpr1aap.R438H within endocardium causes a reduced AV valve area, a downregulation of Wnt/ß-catenin signalling at the AV canal, and growth of additional tissue mass in adult zebrafish hearts. This finding opens the possibility of testing genetic interactions between BMPR1A and other candidate genes within linkage region 1 which may provide a first step towards unravelling more complex genetic patterns in cardiovascular disease aetiology. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-39648-7 SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER -