TY  - JOUR
A1  - Delfan, Maryam
A1  - Vahed, Alieh
A1  - Bishop, David
A1  - Juybari, Raheleh Amadeh
A1  - Laher, Ismail
A1  - Saeidi, Ayoub
A1  - Granacher, Urs
A1  - Zouhal, Hassane
T1  - Effects of two workload-matched high-intensity interval training protocols on regulatory factors associated with mitochondrial biogenesis in the soleus muscle of diabetic rats
JF  - Frontiers in Physiology
N2  - Aims: High intensity interval training (HIIT) improves mitochondrial characteristics. This study compared the impact of two workload-matched high intensity interval training (HIIT) protocols with different work:recovery ratios on regulatory factors related to mitochondrial biogenesis in the soleus muscle of diabetic rats.

Materials and methods: Twenty-four Wistar rats were randomly divided into four equal-sized groups: non-diabetic control, diabetic control (DC), diabetic with long recovery exercise [4–5 × 2-min running at 80%–90% of the maximum speed reached with 2-min of recovery at 40% of the maximum speed reached (DHIIT1:1)], and diabetic with short recovery exercise (5–6 × 2-min running at 80%–90% of the maximum speed reached with 1-min of recovery at 30% of the maximum speed reached [DHIIT2:1]). Both HIIT protocols were completed five times/week for 4 weeks while maintaining equal running distances in each session.

Results: Gene and protein expressions of PGC-1α, p53, and citrate synthase of the muscles increased significantly following DHIIT1:1 and DHIIT2:1 compared to DC (p ˂ 0.05). Most parameters, except for PGC-1α protein (p = 0.597), were significantly higher in DHIIT2:1 than in DHIIT1:1 (p ˂ 0.05). Both DHIIT groups showed significant increases in maximum speed with larger increases in DHIIT2:1 compared with DHIIT1:1.

Conclusion: Our findings indicate that both HIIT protocols can potently up-regulate gene and protein expression of PGC-1α, p53, and CS. However, DHIIT2:1 has superior effects compared with DHIIT1:1 in improving mitochondrial adaptive responses in diabetic rats.
KW  - diabetes mellitus
KW  - muscle metabolism
KW  - time-efficient exercise
KW  - mitochondrial adaptation
KW  - exercise training
Y1  - 2022
U6  - https://doi.org/10.3389/fphys.2022.927969
SN  - 1664-042X
SP  - 1
EP  - 12
PB  - Frontiers
CY  - Lausanne, Schweiz
ER  -