TY  - JOUR
A1  - Lokstein, Heiko
A1  - Krikunova, Maria
A1  - Teuchner, Klaus
A1  - Voigt, Bernd
T1  - Elucidation of structure-function relationships in photosynthetic light-harvesting antenna complexes by non-linear polarization spectroscopy in the frequency domain (NLPF)
JF  - Journal of plant physiology : biochemistry, physiology, molecular biology and biotechnology of plants
N2  - Photosynthetically active pigments are usually organized into pigment-protein complexes. These include light-harvesting antenna complexes (LHCs) and reaction centers. Site energies of the bound pigments are determined by interactions with their environment, i.e., by pigment-protein as well as pigment-pigment interactions. Thus, resolution of spectral substructures of the pigment-protein complexes may provide valuable insight into structure-function relationships.
 By means of conventional (linear) and time-resolved spectroscopic techniques, however, it is often difficult to resolve the spectral substructures of complex pigment-protein assemblies. Nonlinear polarization spectroscopy in the frequency domain (NLPF) is shown to be a valuable technique in this regard. Based on initial experimental work with purple bacterial antenna complexes as well as model systems NLPF has been extended to analyse the substructure(s) of very complex spectra, including analyses of interactions between chlorophylls and "optically dark" states of carotenoids in LHCs. The paper reviews previous work and outlines perspectives regarding the application of NLPF spectroscopy to disentangle structure-function relationships in pigment-protein complexes.
KW  - Excitonic interactions
KW  - Laser spectroscopy
KW  - Light-harvesting complex
KW  - Nonlinear polarization spectroscopy in the frequency domain
KW  - Pigment-pigment interactions
Y1  - 2011
U6  - https://doi.org/10.1016/j.jplph.2010.12.012
SN  - 0176-1617
VL  - 168
IS  - 12
SP  - 1488
EP  - 1496
PB  - Elsevier
CY  - Jena
ER  -