TY  - JOUR
A1  - Blankenburg, Stefanie
A1  - Balfanz, Sabine
A1  - Hayashi, Y.
A1  - Shigenobu, S.
A1  - Miura, T.
A1  - Baumann, Otto
A1  - Baumann, Arnd
A1  - Blenau, Wolfgang
T1  - Cockroach GABA(B) receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution
JF  - Neuropharmacology
N2  - gamma-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABA(A) receptors or metabotropic GABA(B) receptors. GABA(B) receptors regulate, via Gi/o, G-proteins, ion channels, and adenylyl cyclases. In humans, GABA(B) receptor subtypes are involved in the etiology of neurologic and psychiatric disorders. In arthropods, however, these members of the G-protein-coupled receptor family are only inadequately characterized. Interestingly, physiological data have revealed important functions of GABA(B) receptors in the American cockroach, Periplaneta americana. We have cloned cDNAs coding for putative GABA(B) receptor subtypes 1 and 2 of P. americana (PeaGB1 and PeaGB2). When both receptor proteins are co-expressed in mammalian cells, activation of the receptor heteromer with GABA leads to a dose-dependent decrease in cAMP production. The pharmacological profile differs from that of mammalian and Drosophila GABA(B) receptors. Western blot analyses with polyclonal antibodies have revealed the expression of PeaGB1 and PeaGB2 in the CNS of the American cockroach. In addition to the widespread distribution in the brain, PeaGB1 is expressed in salivary glands and male accessory glands. Notably, PeaGB1-like immunoreactivity has been detected in the GABAergic salivary neuron 2, suggesting that GABA(B) receptors act as autoreceptors in this neuron.
KW  - GABA(B) receptor
KW  - G-protein-coupled receptor
KW  - Periplaneta americana
KW  - Central nervous system
KW  - Adenylyl cyclase
KW  - Salivary gland
Y1  - 2015
U6  - https://doi.org/10.1016/j.neuropharm.2014.08.022
SN  - 0028-3908
SN  - 1873-7064
VL  - 88
SP  - 134
EP  - 144
PB  - Elsevier
CY  - Oxford
ER  -