TY  - JOUR
A1  - Heindorff, Kristoffer
A1  - Blenau, Wolfgang
A1  - Walz, Bernd
A1  - Baumann, Otto
T1  - Characterization of a Ca2+/calmodulin-dependent AC1 adenylyl cyclase in a non-neuronal tissue, the blowfly salivary gland
JF  - Cell calcium
N2  - Crosstalk between intracellular signalling pathways is a functionally important and widespread phenomenon in cell physiology across phyla. In the salivary gland of the blowfly, serotonin induces fluid secretion via parallel activation of both the InsP(3)/Ca2+ and the cAMP/PKA signalling pathways, which interact on multiple levels. We have determined the molecular identity of a link between both pathways that mediates a Ca2+-dependent rise of intracellular cAMP. Whereas hydrolysis of cAMP via phosphodiesterases is largely independent of Ca2+, cAMP synthesis by adenylyl cyclases (AC) is potentiated in a Ca2+/calmodulin (Ca2+/CaM)-dependent manner. The existence of a Ca2+/CaM-dependent AC is supported by physiological data and a molecular approach. We have cloned Cv rutabaga cDNA, encoding the first blowfly AC, and confirmed its expression in the salivary gland via reverse transcription followed by polymerase chain reaction. The putative gene product of Cv rutabaga is a Ca2+/CaM-dependent type I AC and shows highest homology to Rutabaga from Drosophila. Thus, a Ca2+/CaM-dependent AC serves as a link between the InsP(3)/Ca2+ and the cAMP/PKA signalling pathways in the salivary gland of the blowfly and might be important for the amplification and optimization of the secretory response.
KW  - Adenylyl cyclase
KW  - Phosphodiesterase
KW  - Crosstalk
KW  - Ca2+
KW  - cAMP
KW  - Intracellular signalling
KW  - Salivary gland
KW  - Calliphora vicina
KW  - Rutabaga
Y1  - 2012
U6  - https://doi.org/10.1016/j.ceca.2012.04.016
SN  - 0143-4160
VL  - 52
IS  - 2
SP  - 103
EP  - 112
PB  - Churchill Livingstone
CY  - Edinburgh
ER  - 
TY  - JOUR
A1  - Blankenburg, Stefanie
A1  - Balfanz, Sabine
A1  - Hayashi, Y.
A1  - Shigenobu, S.
A1  - Miura, T.
A1  - Baumann, Otto
A1  - Baumann, Arnd
A1  - Blenau, Wolfgang
T1  - Cockroach GABA(B) receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution
JF  - Neuropharmacology
N2  - gamma-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABA(A) receptors or metabotropic GABA(B) receptors. GABA(B) receptors regulate, via Gi/o, G-proteins, ion channels, and adenylyl cyclases. In humans, GABA(B) receptor subtypes are involved in the etiology of neurologic and psychiatric disorders. In arthropods, however, these members of the G-protein-coupled receptor family are only inadequately characterized. Interestingly, physiological data have revealed important functions of GABA(B) receptors in the American cockroach, Periplaneta americana. We have cloned cDNAs coding for putative GABA(B) receptor subtypes 1 and 2 of P. americana (PeaGB1 and PeaGB2). When both receptor proteins are co-expressed in mammalian cells, activation of the receptor heteromer with GABA leads to a dose-dependent decrease in cAMP production. The pharmacological profile differs from that of mammalian and Drosophila GABA(B) receptors. Western blot analyses with polyclonal antibodies have revealed the expression of PeaGB1 and PeaGB2 in the CNS of the American cockroach. In addition to the widespread distribution in the brain, PeaGB1 is expressed in salivary glands and male accessory glands. Notably, PeaGB1-like immunoreactivity has been detected in the GABAergic salivary neuron 2, suggesting that GABA(B) receptors act as autoreceptors in this neuron.
KW  - GABA(B) receptor
KW  - G-protein-coupled receptor
KW  - Periplaneta americana
KW  - Central nervous system
KW  - Adenylyl cyclase
KW  - Salivary gland
Y1  - 2015
U6  - https://doi.org/10.1016/j.neuropharm.2014.08.022
SN  - 0028-3908
SN  - 1873-7064
VL  - 88
SP  - 134
EP  - 144
PB  - Elsevier
CY  - Oxford
ER  -