TY  - JOUR
A1  - Vergani, Marco
A1  - Carminati, Marco
A1  - Ferrari, Giorgio
A1  - Landini, Ettore
A1  - Caviglia, Claudia
A1  - Heiskanen, Arto
A1  - Comminges, Clement
A1  - Zor, Kinga
A1  - Sabourin, David
A1  - Dufva, Martin
A1  - Dimaki, Maria
A1  - Raiteri, Roberto
A1  - Wollenberger, Ursula
A1  - Emneus, Jenny
A1  - Sampietro, Marco
T1  - Multichannel bipotentiostat integrated with a microfluidic platform for electrochemical real-time monitoring of cell cultures
JF  - IEEE Transactions on biomedical circuits and systems
N2  - An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system. A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise. Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.
KW  - Electrochemical measurements
KW  - impedance spectroscopy
KW  - microfluidics
KW  - multichannel potentiostat
KW  - stem cell monitoring
Y1  - 2012
U6  - https://doi.org/10.1109/TBCAS.2012.2187783
SN  - 1932-4545
VL  - 6
IS  - 5
SP  - 498
EP  - 507
PB  - Inst. of Electr. and Electronics Engineers
CY  - Piscataway
ER  - 
TY  - JOUR
A1  - Zor, K.
A1  - Heiskanen, A.
A1  - Caviglia, Claudia
A1  - Vergani, M.
A1  - Landini, E.
A1  - Shah, F.
A1  - Carminati, Marco
A1  - Martinez-Serrano, A.
A1  - Ramos Moreno, T.
A1  - Kokaia, M.
A1  - Benayahu, Dafna
A1  - Keresztes, Zs.
A1  - Papkovsky, D.
A1  - Wollenberger, Ursula
A1  - Svendsen, W. E.
A1  - Dimaki, M.
A1  - Ferrari, G.
A1  - Raiteri, R.
A1  - Sampietro, M.
A1  - Dufva, M.
A1  - Emneus, Jenny
T1  - A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection
JF  - RSC Advances
N2  - Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.
Y1  - 2014
U6  - https://doi.org/10.1039/c4ra12632g
SN  - 2046-2069
VL  - 4
IS  - 109
SP  - 63761
EP  - 63771
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Zór, K.
A1  - Heiskanen, A.
A1  - Caviglia, Claudia
A1  - Vergani, M.
A1  - Landini, E.
A1  - Shah, F.
A1  - Carminati, Marco
A1  - Martínez-Serrano, A.
A1  - Ramos Moreno, T.
A1  - Kokaia, M.
A1  - Benayahu, Dafna
A1  - Keresztes, Zs.
A1  - Papkovsky, D.
A1  - Wollenberger, Ursula
A1  - Svendsen, W. E.
A1  - Dimaki, M.
A1  - Ferrari, G.
A1  - Raiteri, R.
A1  - Sampietro, M.
A1  - Dufva, M.
A1  - Emnéus, J.
T1  - A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection
N2  - Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 289 
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-99492
ER  - 
TY  - JOUR
A1  - Acero, F.
A1  - Aloisio, R.
A1  - Amans, J.
A1  - Amato, Elena
A1  - Antonelli, L. A.
A1  - Aramo, C.
A1  - Armstrong, T.
A1  - Arqueros, F.
A1  - Asano, Katsuaki
A1  - Ashley, M.
A1  - Backes, M.
A1  - Balazs, C.
A1  - Balzer, A.
A1  - Bamba, Aya
A1  - Barkov, Maxim
A1  - Barrio, J. A.
A1  - Benbow, Wystan
A1  - Bernloehr, K.
A1  - Beshley, V.
A1  - Bigongiari, C.
A1  - Biland, A.
A1  - Bilinsky, A.
A1  - Bissaldi, Elisabetta
A1  - Biteau, J.
A1  - Blanch, O.
A1  - Blasi, P.
A1  - Blazek, J.
A1  - Boisson, C.
A1  - Bonanno, G.
A1  - Bonardi, A.
A1  - Bonavolonta, C.
A1  - Bonnoli, G.
A1  - Braiding, C.
A1  - Brau-Nogue, S.
A1  - Bregeon, J.
A1  - Brown, A. M.
A1  - Bugaev, V.
A1  - Bulgarelli, A.
A1  - Bulik, T.
A1  - Burton, Michael
A1  - Burtovoi, A.
A1  - Busetto, G.
A1  - Bottcher, M.
A1  - Cameron, R.
A1  - Capalbi, M.
A1  - Caproni, Anderson
A1  - Caraveo, P.
A1  - Carosi, R.
A1  - Cascone, E.
A1  - Cerruti, M.
A1  - Chaty, Sylvain
A1  - Chen, A.
A1  - Chen, X.
A1  - Chernyakova, M.
A1  - Chikawa, M.
A1  - Chudoba, J.
A1  - Cohen-Tanugi, J.
A1  - Colafrancesco, S.
A1  - Conforti, V.
A1  - Contreras, J. L.
A1  - Costa, A.
A1  - Cotter, G.
A1  - Covino, Stefano
A1  - Covone, G.
A1  - Cumani, P.
A1  - Cusumano, G.
A1  - Daniel, M.
A1  - Dazzi, F.
A1  - De Angelis, A.
A1  - De Cesare, G.
A1  - De Franco, A.
A1  - De Frondat, F.
A1  - Dal Pino, E. M. de Gouveia
A1  - De Lisio, C.
A1  - Lopez, R. de los Reyes
A1  - De Lotto, B.
A1  - de Naurois, M.
A1  - De Palma, F.
A1  - Del Santo, M.
A1  - Delgado, C.
A1  - della Volpe, D.
A1  - Di Girolamo, T.
A1  - Di Giulio, C.
A1  - Di Pierro, F.
A1  - Di Venere, L.
A1  - Doro, M.
A1  - Dournaux, J.
A1  - Dumas, D.
A1  - Dwarkadas, Vikram V.
A1  - Diaz, C.
A1  - Ebr, J.
A1  - Egberts, Kathrin
A1  - Einecke, S.
A1  - Elsaesser, D.
A1  - Eschbach, S.
A1  - Falceta-Goncalves, D.
A1  - Fasola, G.
A1  - Fedorova, E.
A1  - Fernandez-Barral, A.
A1  - Ferrand, Gilles
A1  - Fesquet, M.
A1  - Fiandrini, E.
A1  - Fiasson, A.
A1  - Filipovic, Miroslav D.
A1  - Fioretti, V.
A1  - Font, L.
A1  - Fontaine, Gilles
A1  - Franco, F. J.
A1  - Freixas Coromina, L.
A1  - Fujita, Yutaka
A1  - Fukui, Y.
A1  - Funk, S.
A1  - Forster, A.
A1  - Gadola, A.
A1  - Lopez, R. Garcia
A1  - Garczarczyk, M.
A1  - Giglietto, N.
A1  - Giordano, F.
A1  - Giuliani, A.
A1  - Glicenstein, J.
A1  - Gnatyk, R.
A1  - Goldoni, P.
A1  - Grabarczyk, T.
A1  - Graciani, R.
A1  - Graham, J.
A1  - Grandi, P.
A1  - Granot, Jonathan
A1  - Green, A. J.
A1  - Griffiths, S.
A1  - Gunji, S.
A1  - Hakobyan, H.
A1  - Hara, S.
A1  - Hassan, T.
A1  - Hayashida, M.
A1  - Heller, M.
A1  - Helo, J. C.
A1  - Hinton, J.
A1  - Hnatyk, B.
A1  - Huet, J.
A1  - Huetten, M.
A1  - Humensky, T. B.
A1  - Hussein, M.
A1  - Horandel, J.
A1  - Ikeno, Y.
A1  - Inada, T.
A1  - Inome, Y.
A1  - Inoue, S.
A1  - Inoue, T.
A1  - Inoue, Y.
A1  - Ioka, K.
A1  - Iori, Maurizio
A1  - Jacquemier, J.
A1  - Janecek, P.
A1  - Jankowsky, D.
A1  - Jung, I.
A1  - Kaaret, P.
A1  - Katagiri, H.
A1  - Kimeswenger, S.
A1  - Kimura, Shigeo S.
A1  - Knodlseder, J.
A1  - Koch, B.
A1  - Kocot, J.
A1  - Kohri, K.
A1  - Komin, N.
A1  - Konno, Y.
A1  - Kosack, K.
A1  - Koyama, S.
A1  - Kraus, Michaela
A1  - Kubo, Hidetoshi
A1  - Mezek, G. Kukec
A1  - Kushida, J.
A1  - La Palombara, N.
A1  - Lalik, K.
A1  - Lamanna, G.
A1  - Landt, H.
A1  - Lapington, J.
A1  - Laporte, P.
A1  - Lee, S.
A1  - Lees, J.
A1  - Lefaucheur, J.
A1  - Lenain, J. -P.
A1  - Leto, Giuseppe
A1  - Lindfors, E.
A1  - Lohse, T.
A1  - Lombardi, S.
A1  - Longo, F.
A1  - Lopez, M.
A1  - Lucarelli, F.
A1  - Luque-Escamilla, Pedro Luis
A1  - Lopez-Coto, R.
A1  - Maccarone, M. C.
A1  - Maier, G.
A1  - Malaguti, G.
A1  - Mandat, D.
A1  - Maneva, G.
A1  - Mangano, S.
A1  - Marcowith, Alexandre
A1  - Marti, J.
A1  - Martinez, M.
A1  - Martinez, G.
A1  - Masuda, S.
A1  - Maurin, G.
A1  - Maxted, N.
A1  - Melioli, Claudio
A1  - Mineo, T.
A1  - Mirabal, N.
A1  - Mizuno, T.
A1  - Moderski, R.
A1  - Mohammed, M.
A1  - Montaruli, T.
A1  - Moralejo, A.
A1  - Mori, K.
A1  - Morlino, G.
A1  - Morselli, A.
A1  - Moulin, Emmanuel
A1  - Mukherjee, R.
A1  - Mundell, C.
A1  - Muraishi, H.
A1  - Murase, Kohta
A1  - Nagataki, Shigehiro
A1  - Nagayoshi, T.
A1  - Naito, T.
A1  - Nakajima, D.
A1  - Nakamori, T.
A1  - Nemmen, R.
A1  - Niemiec, Jacek
A1  - Nieto, D.
A1  - Nievas-Rosillo, M.
A1  - Nikolajuk, M.
A1  - Nishijima, K.
A1  - Noda, K.
A1  - Nogues, L.
A1  - Nosek, D.
A1  - Novosyadlyj, B.
A1  - Nozaki, S.
A1  - Ohira, Yutaka
A1  - Ohishi, M.
A1  - Ohm, S.
A1  - Okumura, A.
A1  - Ong, R. A.
A1  - Orito, R.
A1  - Orlati, A.
A1  - Ostrowski, M.
A1  - Oya, I.
A1  - Padovani, Marco
A1  - Palacio, J.
A1  - Palatka, M.
A1  - Paredes, Josep M.
A1  - Pavy, S.
A1  - Persic, M.
A1  - Petrucci, P.
A1  - Petruk, Oleh
A1  - Pisarski, A.
A1  - Pohl, Martin
A1  - Porcelli, A.
A1  - Prandini, E.
A1  - Prast, J.
A1  - Principe, G.
A1  - Prouza, M.
A1  - Pueschel, Elisa
A1  - Puelhofer, G.
A1  - Quirrenbach, A.
A1  - Rameez, M.
A1  - Reimer, O.
A1  - Renaud, M.
A1  - Ribo, M.
A1  - Rico, J.
A1  - Rizi, V.
A1  - Rodriguez, J.
A1  - Fernandez, G. Rodriguez
A1  - Rodriguez Vazquez, J. J.
A1  - Romano, Patrizia
A1  - Romeo, G.
A1  - Rosado, J.
A1  - Rousselle, J.
A1  - Rowell, G.
A1  - Rudak, B.
A1  - Sadeh, I.
A1  - Safi-Harb, S.
A1  - Saito, T.
A1  - Sakaki, N.
A1  - Sanchez, D.
A1  - Sangiorgi, P.
A1  - Sano, H.
A1  - Santander, M.
A1  - Sarkar, S.
A1  - Sawada, M.
A1  - Schioppa, E. J.
A1  - Schoorlemmer, H.
A1  - Schovanek, P.
A1  - Schussler, F.
A1  - Sergijenko, O.
A1  - Servillat, M.
A1  - Shalchi, A.
A1  - Shellard, R. C.
A1  - Siejkowski, H.
A1  - Sillanpaa, A.
A1  - Simone, D.
A1  - Sliusar, V.
A1  - Sol, H.
A1  - Stanic, S.
A1  - Starling, R.
A1  - Stawarz, L.
A1  - Stefanik, S.
A1  - Stephan, M.
A1  - Stolarczyk, T.
A1  - Szanecki, M.
A1  - Szepieniec, T.
A1  - Tagliaferri, G.
A1  - Tajima, H.
A1  - Takahashi, M.
A1  - Takeda, J.
A1  - Tanaka, M.
A1  - Tanaka, S.
A1  - Tejedor, L. A.
A1  - Telezhinsky, Igor O.
A1  - Temnikov, P.
A1  - Terada, Y.
A1  - Tescaro, D.
A1  - Teshima, M.
A1  - Testa, V.
A1  - Thoudam, S.
A1  - Tokanai, F.
A1  - Torres, D. F.
A1  - Torresi, E.
A1  - Tosti, G.
A1  - Townsley, C.
A1  - Travnicek, P.
A1  - Trichard, C.
A1  - Trifoglio, M.
A1  - Tsujimoto, S.
A1  - Vagelli, V.
A1  - Vallania, P.
A1  - Valore, L.
A1  - van Driel, W.
A1  - van Eldik, C.
A1  - Vandenbroucke, Justin
A1  - Vassiliev, V.
A1  - Vecchi, M.
A1  - Vercellone, Stefano
A1  - Vergani, S.
A1  - Vigorito, C.
A1  - Vorobiov, S.
A1  - Vrastil, M.
A1  - Vazquez Acosta, M. L.
A1  - Wagner, S. J.
A1  - Wagner, R.
A1  - Wakely, S. P.
A1  - Walter, R.
A1  - Ward, J. E.
A1  - Watson, J. J.
A1  - Weinstein, A.
A1  - White, M.
A1  - White, R.
A1  - Wierzcholska, A.
A1  - Wilcox, P.
A1  - Williams, D. A.
A1  - Wischnewski, R.
A1  - Wojcik, P.
A1  - Yamamoto, T.
A1  - Yamamoto, H.
A1  - Yamazaki, Ryo
A1  - Yanagita, S.
A1  - Yang, L.
A1  - Yoshida, T.
A1  - Yoshida, M.
A1  - Yoshiike, S.
A1  - Yoshikoshi, T.
A1  - Zacharias, M.
A1  - Zampieri, L.
A1  - Zanin, R.
A1  - Zavrtanik, M.
A1  - Zavrtanik, D.
A1  - Zdziarski, A.
A1  - Zech, Alraune
A1  - Zechlin, Hannes
A1  - Zhdanov, V.
A1  - Ziegler, A.
A1  - Zorn, J.
T1  - Prospects for Cherenkov Telescope Array Observations of the Young Supernova Remnant RX J1713.7-3946
JF  - The astrophysical journal : an international review of spectroscopy and astronomical physics
N2  - We perform simulations for future Cherenkov Telescope Array (CTA) observations of RX J1713.7-3946, a young supernova remnant (SNR) and one of the brightest sources ever discovered in very high energy (VHE) gamma rays. Special attention is paid to exploring possible spatial (anti) correlations of gamma rays with emission at other wavelengths, in particular X-rays and CO/H I emission. We present a series of simulated images of RX J1713.7-3946 for CTA based on a set of observationally motivated models for the gamma-ray emission. In these models, VHE gamma rays produced by high-energy electrons are assumed to trace the nonthermal X-ray emission observed by XMM-Newton, whereas those originating from relativistic protons delineate the local gas distributions. The local atomic and molecular gas distributions are deduced by the NANTEN team from CO and H I observations. Our primary goal is to show how one can distinguish the emission mechanism(s) of the gamma rays (i.e., hadronic versus leptonic, or a mixture of the two) through information provided by their spatial distribution, spectra, and time variation. This work is the first attempt to quantitatively evaluate the capabilities of CTA to achieve various proposed scientific goals by observing this important cosmic particle accelerator.
KW  - cosmic rays
KW  - gamma rays: ISM
KW  - ISM: individual objects (RX J1713.7-3946, G347.3-0.5)
Y1  - 2017
U6  - https://doi.org/10.3847/1538-4357/aa6d67
SN  - 0004-637X
SN  - 1538-4357
VL  - 840
IS  - 2
PB  - IOP Publ. Ltd.
CY  - Bristol
ER  -