TY - JOUR A1 - Wels, Nicolett A1 - Lenz, Petra A1 - Christians, Heiko A1 - Findeklee, Ulrike A1 - Zimmermann, Matthias A1 - Mangelsdorf, Birgit A1 - Horn-Conrad, Antje A1 - Görlich, Petra A1 - Peter, Andreas A1 - Rist, Juri A1 - Voigt, Juliane A1 - Eman, Friderike A1 - Micka, Bettina T1 - Portal = Glaube und Wissen: Religionswissenschaften im säkularen Staat BT - Das Potsdamer Universitätsmagazin N2 - Aus dem Inhalt: - Glauben und Wissen: Religionswissenschaften im säkularen Staat - Geheimnisse des Geistes - Mit den Dänen auf Du und Du T3 - Portal: Das Potsdamer Universitätsmagazin - 04/2010 Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-440225 SN - 1618-6893 IS - 04/2010 ER - TY - JOUR A1 - Cui, Pin A1 - Löber, Ulrike A1 - Alquezar-Planas, David E. A1 - Ishida, Yasuko A1 - Courtiol, Alexandre A1 - Timms, Peter A1 - Johnson, Rebecca N. A1 - Lenz, Dorina A1 - Helgen, Kristofer M. A1 - Roca, Alfred L. A1 - Hartman, Stefanie A1 - Greenwood, Alex D. T1 - Comprehensive profiling of retroviral integration sites using target enrichment methods from historical koala samples without an assembled reference genome JF - PeerJ N2 - Background. Retroviral integration into the host germline results in permanent viral colonization of vertebrate genomes. The koala retrovirus (KoRV) is currently invading the germline of the koala (Phascolarctos cinereus) and provides a unique opportunity for studying retroviral endogenization. Previous analysis of KoRV integration patterns in modern koalas demonstrate that they share integration sites primarily if they are related, indicating that the process is currently driven by vertical transmission rather than infection. However, due to methodological challenges, KoRV integrations have not been comprehensively characterized. Results. To overcome these challenges, we applied and compared three target enrichment techniques coupled with next generation sequencing (NGS) and a newly customized sequence-clustering based computational pipeline to determine the integration sites for 10 museum Queensland and New South Wales (NSW) koala samples collected between the 1870s and late 1980s. A secondary aim of this study sought to identify common integration sites across modern and historical specimens by comparing our dataset to previously published studies. Several million sequences were processed, and the KoRV integration sites in each koala were characterized. Conclusions. Although the three enrichment methods each exhibited bias in integration site retrieval, a combination of two methods, Primer Extension Capture and hybridization capture is recommended for future studies on historical samples. Moreover, identification of integration sites shows that the proportion of integration sites shared between any two koalas is quite small. KW - Integration sites KW - Retroviral endogenization KW - KoRV KW - Target enrichment KW - Clustering Y1 - 2016 U6 - https://doi.org/10.7717/peerj.1847 SN - 2167-8359 VL - 4 PB - PeerJ Inc. CY - London ER - TY - JOUR A1 - Hahnewald, Rita A1 - Leimkühler, Silke A1 - Vilaseca, Antonia A1 - Acquaviva-Bourdain, Cecile A1 - Lenz, Ulrike A1 - Reiss, Jochen T1 - A novel MOCS2 mutation reveals coordinated expression of the small and large subunit of molybdopterin synthase JF - Molecular genetics and metabolism N2 - The small and large subunits of molybdopterin (MPT) synthase (MOCS2A and MOCS2B), are both encoded by the MOCS2 gene in overlapping and shifted open reading frames (ORFs), which is a highly unusual structure for eukaryotes. Theoretical analysis of genomic sequences suggested that the expression of these overlapping ORFs is facilitated by the use of alternate first exons leading to alternative transcripts. Here, we confirm the existence of these overlapping transcripts experimentally. Further, we identified a deletion in a molybdenum cofactor deficient patient, which removes the start codon for the small subunit (MOCS2A). We observed undisturbed production of both transcripts, while Western blot analysis demonstrated that MOCS2B, the large subunit, is unstable in the absence of MOCS2A. This reveals new insights into the expression of this evolutionary ancient anabolic system. KW - molybdenum cofactor deficiency KW - MOCS2 KW - overlapping reading frames Y1 - 2006 U6 - https://doi.org/10.1016/j.ymgme.2006.04.008 SN - 1096-7192 VL - 89 IS - 3 SP - 210 EP - 213 PB - Elsevier CY - San Diego ER - TY - BOOK A1 - Möring, Sebastian A1 - Pohl, Manuela A1 - Riemer, Nathanael A1 - Distelmeyer, Jan A1 - Lenz, Petra A1 - Fritsch, Melanie A1 - Lucke, Ulrike A1 - Moebert, Tobias A1 - Zoerner, Dietmar A1 - Ernst, Sebastian A1 - Wienecke, Maik A1 - Montag, Annegret A1 - Goetz, Ilka ED - Möring, Sebastian ED - Pohl, Manuela ED - Riemer, Nathanael T1 - Didaktik des digitalen Spielens T3 - DIGAREC Series N2 - Welche Eigenschaften machen das Computerspiel zum geeigneten Medium, das den pädagogischen Einsatz im Unterricht bereichern kann? Welche Computerspiele bieten welche Möglichkeiten zur Auseinandersetzung mit welchen Themen? Wie kann das Computerspiel auch im schulischen Umfeld den für den Lernprozess so wichtigen Lebensweltbezug herstellen? Diese und viele weitere Fragen beantworten die Autor*innen des Bandes „Didaktik des digitalen Spielens“. Dafür begeben sie sich in einen Dialog der Wissenschaftsdisziplinen, leiten Möglichkeiten zum Einsatz von Computerspielen ab und werten Erfahrungen mit dem Einsatz von Computerspielen – auch in der Lehrendenbildung – aus. Mit ihren verschiedenen Zugängen zu Fragestellungen rund um eine „Didaktik des digitalen Spielens“ liefern sie einen Beitrag zu einem Diskurs, der besonders in Zeiten von Distanzunterricht notwendig und folgerichtig geführt werden muss. Die im Rahmen der gleichnamigen interdisziplinären Ringvorlesung im Wintersemester 2018/19 an der Universität Potsdam gehaltenen Vorträge sind durch die Diskussionen mit Studierenden geprägt und ausgewertet worden, so dass sie in der nun veröffentlichten Form auf mehreren Ebenen einen mehrperspektivischen Blick auf den Gegenstand „Computerspiel im Unterricht“ legen. T3 - DIGAREC Series - 09 KW - Bildung KW - Computerspiele KW - Didaktik KW - Medien KW - Unterricht KW - Pädadgogik KW - computer games KW - didactics KW - education KW - media KW - teaching KW - pedagogy Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-509570 SN - 978-3-86956-511-8 SN - 1867-6219 SN - 1867-6227 IS - 09 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Andreev, Andrei A1 - Nazarova, Larisa B. A1 - Lenz, Marlene M. A1 - Böhmer, Thomas A1 - Syrykh, Ludmila A1 - Wagner, Bernd A1 - Melles, Martin A1 - Pestryakova, Luidmila A. A1 - Herzschuh, Ulrike T1 - Late Quaternary paleoenvironmental reconstructions from sediments of Lake Emanda (Verkhoyansk Mountains, East Siberia) JF - Journal of quaternary science : JQS N2 - Continuous pollen and chironomid records from Lake Emanda (65 degrees 17'N, 135 degrees 45'E) provide new insights into the Late Quaternary environmental history of the Yana Highlands (Yakutia). Larch forest with shrubs (alders, pines, birches) dominated during the deposition of the lowermost sediments suggesting its Early Weichselian [Marine Isotope Stage (MIS) 5] age. Pollen- and chironomid-based climate reconstructions suggest July temperatures (T-July) slightly lower than modern. Gradually increasing amounts of herb pollen and cold stenotherm chironomid head capsules reflect cooler and drier environments, probably during the termination of MIS 5. T-July dropped to 8 degrees C. Mostly treeless vegetation is reconstructed during MIS 3. Tundra and steppe communities dominated during MIS 2. Shrubs became common after similar to 14.5 ka BP but herb-dominated habitats remained until the onset of the Holocene. Larch forests with shrub alder and dwarf birch dominated after the Holocene onset, ca. 11.7 ka BP. Decreasing amounts of shrub pollen during the Lateglacial are assigned to the Older Dryas and Younger Dryas with T-July similar to 7.5 degrees C. T-July increased up to 13 degrees C. Shrub stone pine was present after similar to 7.5 ka BP. The vegetation has been similar to modern since ca. 5.8 ka BP. Chironomid diversity and concentration in the sediments increased towards the present day, indicating the development of richer hydrobiological communities in response to the Holocene thermal maximum. KW - chironomids KW - environmental reconstructions KW - Late Quaternary KW - pollen Y1 - 2022 U6 - https://doi.org/10.1002/jqs.3419 SN - 0267-8179 SN - 1099-1417 VL - 37 IS - 5 SP - 884 EP - 899 PB - Wiley CY - New York, NY [u.a.] ER -