TY  - JOUR
A1  - Yu, Yanjun
A1  - Wu, Shenjie
A1  - Nowak, Jacqueline
A1  - Wang, Guangda
A1  - Han, Libo
A1  - Feng, Zhidi
A1  - Mendrinna, Amelie
A1  - Ma, Yinping
A1  - Wang, Huan
A1  - Zhang, Xiaxia
A1  - Tian, Juan
A1  - Dong, Li
A1  - Nikoloski, Zoran
A1  - Persson, Staffan
A1  - Kong, Zhaosheng
T1  - Live-cell imaging of the cytoskeleton in elongating cotton fibres
JF  - Nature plants
N2  - Cotton (Gossypium hirsutum) fibres consist of single cells that grow in a highly polarized manner, assumed to be controlled by the cytoskeleton(1-3). However, how the cytoskeletal organization and dynamics underpin fibre development remains unexplored. Moreover, it is unclear whether cotton fibres expand via tip growth or diffuse growth(2-4). We generated stable transgenic cotton plants expressing fluorescent markers of the actin and microtubule cytoskeleton. Live-cell imaging revealed that elongating cotton fibres assemble a cortical filamentous actin network that extends along the cell axis to finally form actin strands with closed loops in the tapered fibre tip. Analyses of F-actin network properties indicate that cotton fibres have a unique actin organization that blends features of both diffuse and tip growth modes. Interestingly, typical actin organization and endosomal vesicle aggregation found in tip-growing cell apices were not observed in fibre tips. Instead, endomembrane compartments were evenly distributed along the elongating fibre cells and moved bi-directionally along the fibre shank to the fibre tip. Moreover, plus-end tracked microtubules transversely encircled elongating fibre shanks, reminiscent of diffusely growing cells. Collectively, our findings indicate that cotton fibres elongate via a unique tip-biased diffuse growth mode.
Y1  - 2019
U6  - https://doi.org/10.1038/s41477-019-0418-8
SN  - 2055-026X
SN  - 2055-0278
VL  - 5
IS  - 5
SP  - 498
EP  - 504
PB  - Nature Publ. Group
CY  - London
ER  -