TY - JOUR A1 - Anklam, Elke A1 - Behler, Jörg A1 - Dingermann, Theodor A1 - Elsinghorst, Paul A1 - Fischer, Jochen A1 - Esselen, Melanie A1 - Foerster, Christian A1 - Fröhlich, Daniel A1 - Goedel, Werner Andreas A1 - Gregory, Peter A1 - Grimme, Stefan A1 - Hackenberger, Christian A1 - Hansmann, Max A1 - Heppekausen, Johannes A1 - Hasenstab-Riedel, Sebastian A1 - Kirchhoff, Erhard A1 - Kratz, Karl-Ludwig A1 - Krausz, Ferenc A1 - Linker, Torsten A1 - List, Benjamin A1 - Ray, Kallol A1 - Salzer, Reiner A1 - Schubert, Ulrich A1 - Schueth, Ferdi A1 - Schwarz, Helmut A1 - Schwietzke, Uta A1 - Strey, Reinhard A1 - Stumpf, Thorsten A1 - Vaagt, Franziska A1 - Volodkin, Dmitry A1 - Wilke, Guenther A1 - Zass, Engelbert A1 - Zemb, Thomas T1 - Awards JF - Nachrichten aus der Chemie : Zeitschrift der Gesellschaft Deutscher Chemiker Y1 - 2013 U6 - https://doi.org/10.1002/nadc.201390372 SN - 1439-9598 SN - 1868-0054 VL - 61 IS - 11 SP - 1145 EP - 1148 PB - Ges. Dt. Chemiker CY - Frankfurt, Main ER - TY - JOUR A1 - Numberger, Daniela A1 - Riedel, Thomas A1 - McEwen, Gayle A1 - Nübel, Ulrich A1 - Frentrup, Martinique A1 - Schober, Isabel A1 - Bunk, Boyke A1 - Spröer, Cathrin A1 - Overmann, Jörg A1 - Grossart, Hans-Peter A1 - Greenwood, Alex D. T1 - Genomic analysis of three Clostridioides difficile isolates from urban water sources JF - Anaerobe N2 - We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed. (C) 2019 Elsevier Ltd. All rights reserved. KW - Clostridioides difficile KW - Genomes KW - Sediment KW - Wastewater Y1 - 2019 U6 - https://doi.org/10.1016/j.anaerobe.2019.01.002 SN - 1075-9964 SN - 1095-8274 VL - 56 SP - 22 EP - 26 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Gutschmann, Björn A1 - Simões, Matilde Maldonado A1 - Schiewe, Thomas A1 - Schröter, Edith S. A1 - Münzberg, Marvin A1 - Neubauer, Peter A1 - Bockisch, Anika A1 - Riedel, Sebastian Lothar Stefan T1 - Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory- and pilot-scale JF - Microbial biotechnology / Society for Applied Microbiology N2 - Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor-made process development. In this study, a double-jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory-scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 g(PHA) L-1) and a 75% higher space-time yield (0.63 g(PHA) L-1 h(-1)) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real-time by in-line photon density wave spectroscopy. The process robustness was further evaluated during scale-down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two-thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale-down cultivations. Application of this feeding system at 150-L pilot-scale cultivation yielded in 31.5 g(PHA) L-1, which is a promising result for the further scale-up to industrial scale. Y1 - 2022 U6 - https://doi.org/10.1111/1751-7915.14104 SN - 1751-7915 PB - Wiley CY - Hoboken ER -