TY  - JOUR
A1  - Heinken, Thilo
A1  - Schmidt, M.
A1  - von Oheimb, Goddert
A1  - Kriebitzsch, Wolf-Ulrich
A1  - Ellenberg, H.
T1  - Ausbreitung von Pflanzen durch Schalenwild
Y1  - 2005
SN  - 0936-1294 -
ER  - 
TY  - JOUR
A1  - De Frenne, Pieter
A1  - Blondeel, H.
A1  - Brunet, J.
A1  - Caron, M. M.
A1  - Chabrerie, O.
A1  - Cougnon, M.
A1  - Cousins, S. A. O.
A1  - Decocq, G.
A1  - Diekmann, M.
A1  - Graae, B. J.
A1  - Hanley, M. E.
A1  - Heinken, Thilo
A1  - Hermy, M.
A1  - Kolb, A.
A1  - Lenoir, J.
A1  - Liira, J.
A1  - Orczewska, A.
A1  - Shevtsova, A.
A1  - Vanneste, T.
A1  - Verheyen, K.
T1  - Atmospheric nitrogen deposition on petals enhances seed quality of the forest herb Anemone nemorosa
JF  - Plant biology
N2  - Elevated atmospheric input of nitrogen (N) is currently affecting plant biodiversity and ecosystem functioning. The growth and survival of numerous plant species is known to respond strongly to N fertilisation. Yet, few studies have assessed the effects of N deposition on seed quality and reproductive performance, which is an important life-history stage of plants. Here we address this knowledge gap by assessing the effects of atmospheric N deposition on seed quality of the ancient forest herb Anemone nemorosa using two complementary approaches. By taking advantage of the wide spatiotemporal variation in N deposition rates in pan-European temperate and boreal forests over 2years, we detected positive effects of N deposition on the N concentration (percentage N per unit seed mass, increased from 2.8% to 4.1%) and N content (total N mass per seed more than doubled) of A.nemorosa seeds. In a complementary experiment, we applied ammonium nitrate to aboveground plant tissues and the soil surface to determine whether dissolved N sources in precipitation could be incorporated into seeds. Although the addition of N to leaves and the soil surface had no effect, a concentrated N solution applied to petals during anthesis resulted in increased seed mass, seed N concentration and N content. Our results demonstrate that N deposition on the petals enhances bioaccumulation of N in the seeds of A.nemorosa. Enhanced atmospheric inputs of N can thus not only affect growth and population dynamics via root or canopy uptake, but can also influence seed quality and reproduction via intake through the inflorescences.
KW  - Latitudinal gradient
KW  - nitrogen deposition
KW  - nutrient stoichiometry
KW  - seed provisioning
KW  - seed quality
KW  - sexual reproduction
KW  - wood anemone
Y1  - 2018
U6  - https://doi.org/10.1111/plb.12688
SN  - 1435-8603
SN  - 1438-8677
VL  - 20
IS  - 3
SP  - 619
EP  - 626
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Liang, Wei
A1  - Heinrich, Ingo
A1  - Helle, Gerhard
A1  - Linan, Isabel Dorado
A1  - Heinken, Thilo
T1  - Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy
JF  - Dendrochronologia : an interdisciplinary journal of tree-ring science
N2  - A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.
KW  - Wood anatomy
KW  - Cell structures
KW  - Confocal laser scanning microscopy
KW  - CLSM
KW  - Dendrochronology
KW  - Surface preparation
Y1  - 2013
U6  - https://doi.org/10.1016/j.dendro.2012.09.002
SN  - 1125-7865
VL  - 31
IS  - 2
SP  - 140
EP  - 145
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - De Frenne, Pieter
A1  - Graae, Bente J.
A1  - Kolb, Annette
A1  - Shevtsova, Anna
A1  - Baeten, Lander
A1  - Brunet, Jörg
A1  - Chabrerie, Olivier
A1  - Cousins, Sara A. O.
A1  - Decocq, Guillaume
A1  - Dhondt, Rob
A1  - Diekmann, Martin
A1  - Gruwez, Robert
A1  - Heinken, Thilo
A1  - Hermy, Martin
A1  - Oster, Mathias
A1  - Saguez, Robert
A1  - Stanton, Sharon
A1  - Tack, Wesley
A1  - Vanhellemont, Margot
A1  - Verheyen, Kris
T1  - An intraspecific application of the leaf-height-seed ecology strategy scheme to forest herbs along a latitudinal gradient
JF  - Ecography : pattern and diversity in ecology ; research papers forum
N2  - We measured LHS traits in 41 Anemone nemorosa and 44 Milium effusum populations along a 1900-2300 km latitudinal gradient from N France to N Sweden. We then applied multilevel models to identify the effects of regional (temperature, latitude) and local (soil fertility and acidity, overstorey canopy cover) environmental factors on LHS traits.
 Both species displayed a significant 4% increase in plant height with every degree northward shift (almost a two-fold plant height difference between the southernmost and northernmost populations). Neither seed mass nor SLA showed a significant latitudinal cline. Temperature had a large effect on the three LHS traits of Anemone. Latitude, canopy cover and soil nutrients were related to the SLA and plant height of Milium. None of the investigated variables appeared to be related to the seed mass of Milium.
 The variation in LHS traits indicates that the ecological strategy determined by the position of each population in this three-factor triangle is not constant along the latitudinal gradient. The significant increase in plant height suggests greater competitive abilities for both species in the northernmost populations. We also found that the studied environmental factors affected the LHS traits of the two species on various scales: spring-flowering Anemone was affected more by temperature, whereas early-summer flowering Milium was affected more by local and other latitude-related factors. Finally, previously reported cross-species correlations between LHS traits and latitude were generally unsupported by our within-species approach.
Y1  - 2011
U6  - https://doi.org/10.1111/j.1600-0587.2010.06399.x
SN  - 0906-7590
VL  - 34
IS  - 1
SP  - 132
EP  - 140
PB  - Wiley-Blackwell
CY  - Malden
ER  - 
TY  - JOUR
A1  - Reinecke, J.
A1  - Wulf, M.
A1  - Baeten, Lander
A1  - Brunet, J.
A1  - Decocq, G.
A1  - De Frenne, G.
A1  - Diekmann, M.
A1  - Graae, B. J.
A1  - Heinken, Thilo
A1  - Hermy, M.
A1  - Jamoneau, A.
A1  - Lenoir, J.
A1  - Plue, J.
A1  - Orczewska, A.
A1  - Van Calster, H.
A1  - Verheyen, Kris
A1  - Naaf, T.
T1  - Acido- and neutrophilic temperate forest plants display distinct shifts in ecological pH niche across north-western Europe
JF  - Ecography : pattern and diversity in ecology ; research papers forum
N2  - Ecological niches of organisms vary across geographical space, but niche shift patterns between regions and the underlying mechanisms remain largely unexplored. We studied shifts in the pH niche of 42 temperate forest plant species across a latitudinal gradient from northern France to boreo-nemoral Sweden. We asked 1) whether species restrict their niches with increasing latitude as they reach their northern range margin (environmental constraints); 2) whether species expand their niches with increasing latitude as regional plant species richness decreases (competitive release); and 3) whether species shift their niche position toward more acidic sites with increasing latitude as the relative proportion of acidic soils increases (local adaptation). Based on 1458 vegetation plots and corresponding soil pH values, we modelled species response curves using Huisman-Olff-Fresco models. Four niche measures (width, position, left and right border) were compared among regions by randomization tests. We found that with increasing latitude, neutrophilic species tended to retreat from acidic sites, indicating that these species retreat to more favorable sites when approaching their range margin. Alternatively, these species might benefit from enhanced nitrogen deposition on formerly nutrient-poor, acidic sites in southern regions or lag behind in post-glacial recolonization of potential habitats in northern regions. Most acidophilic species extended their niche toward more base-rich sites with increasing latitude, indicating competitive release from neutrophilic species. Alternatively, acidophilic species might benefit from optimal climatic conditions in the north where some have their core distribution area. Shifts in the niche position suggested that local adaptation is of minor importance. We conclude that shifts in the pH niche of temperate forest plants are the rule, but the directions of the niche shifts and possible explanations vary. Our study demonstrates that differentiating between acidophilic and neutrophilic species is crucial to identify general patterns and underlying mechanisms.
Y1  - 2016
U6  - https://doi.org/10.1111/ecog.02051
SN  - 0906-7590
SN  - 1600-0587
VL  - 39
SP  - 1164
EP  - 1175
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Baeten, Lander
A1  - Warton, David I.
A1  - Van Calster, Hans
A1  - De Frenne, Pieter
A1  - Verstraeten, Gorik
A1  - Bonte, Dries
A1  - Bernhardt-Römermann, Markus
A1  - Cornelis, Johnny
A1  - Decocq, Guillaume
A1  - Eriksson, Ove
A1  - Hedl, Radim
A1  - Heinken, Thilo
A1  - Hermy, Martin
A1  - Hommel, Patrick
A1  - Kirby, Keith J.
A1  - Naaf, Tobias
A1  - Petrik, Petr
A1  - Walther, Gian-Reto
A1  - Wulf, Monica
A1  - Verheyen, Kris
T1  - A model-based approach to studying changes in compositional heterogeneity
JF  - Methods in ecology and evolution : an official journal of the British Ecological Society
Y1  - 2014
SN  - 2041-210X
SN  - 2041-2096
VL  - 5
IS  - 2
SP  - 156
EP  - 164
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - De Frenne, P.
A1  - Kolb, Annette
A1  - Graae, Benete Jessen
A1  - Decocq, Guillaume
A1  - Baltora, S.
A1  - De Schrijver, A.
A1  - Brunet, J.
A1  - Chabrerie, Olivier
A1  - Cousins, Sara A. O.
A1  - Dhondt, Rob
A1  - Diekmann, Martin
A1  - Gruwez, R.
A1  - Heinken, Thilo
A1  - Hermy, Martin
A1  - Liira, J.
A1  - Saguez, R.
A1  - Shevtsova, Anna
A1  - Baskin, Carol C.
A1  - Verheyen, Kris
T1  - A latitudinal gradient in seed nutrients of the forest herb Anemone nemorosa
JF  - Plant biology
N2  - The nutrient concentration in seeds determines many aspects of potential success of the sexual reproductive phase of plants, including the seed predation probability, efficiency of seed dispersal and seedling performance. Despite considerable research interest in latitudinal gradients of foliar nutrients, a similar gradient for seeds remains unexplored. We investigated a potential latitudinal gradient in seed nutrient concentrations within the widespread European understorey forest herb Anemone nemorosa L. We sampled seeds of A. nemorosa in 15 populations along a 1900-km long latitudinal gradient at three to seven seed collection dates post-anthesis and investigated the relative effects of growing degree-hours > 5 degrees C, soil characteristics and latitude on seed nutrient concentrations. Seed nitrogen, nitrogen:phosphorus ratio and calcium concentration decreased towards northern latitudes, while carbon:nitrogen ratios increased. When taking differences in growing degree-hours and measured soil characteristics into account and only considering the most mature seeds, the latitudinal decline remained particularly significant for seed nitrogen concentration. We argue that the decline in seed nitrogen concentration can be attributed to northward decreasing seed provisioning due to lower soil nitrogen availability or greater investment in clonal reproduction. This pattern may have large implications for the reproductive performance of this forest herb as the degree of seed provisioning ultimately co-determines seedling survival and reproductive success.
KW  - Collection date
KW  - latitude
KW  - nutrient stoichiometry
KW  - seed nitrogen
KW  - seed predation
KW  - seed provisioning
KW  - sexual reproduction
KW  - wood anemone
Y1  - 2011
U6  - https://doi.org/10.1111/j.1438-8677.2010.00404.x
SN  - 1435-8603
VL  - 13
IS  - 3
SP  - 493
EP  - 501
PB  - Wiley-Blackwell
CY  - Malden
ER  -