TY  - JOUR
A1  - Chakraborty, Sudipta
A1  - Chen, Pan
A1  - Bornhorst, Julia
A1  - Schwerdtle, Tanja
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Bowman, Aaron B.
A1  - Aschner, Michael A.
T1  - Loss of pdr-1/parkin influences Mn homeostasis through altered ferroportin expression in C-elegans
JF  - Metallomics : integrated biometal science
Y1  - 2015
U6  - https://doi.org/10.1039/c5mt00052a
SN  - 1756-5901
SN  - 1756-591X
VL  - 7
IS  - 5
SP  - 847
EP  - 856
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Bzymek, Robert
A1  - Horsthemke, Markus
A1  - Isfort, Katrin
A1  - Mohr, Simon
A1  - Tjaden, Kerstin
A1  - Mueller-Tidow, Carsten
A1  - Thomann, Marlies
A1  - Schwerdtle, Tanja
A1  - Baehler, Martin
A1  - Schwab, Albrecht
A1  - Hanley, Peter J.
T1  - Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho
JF  - Scientific reports
N2  - We recently found that macrophages from RhoA/RhoB double knockout mice had increased motility of the cell body, but severely impaired retraction of the tail and membrane extensions, whereas RhoA-or RhoB-deficient cells exhibited mild phenotypes. Here we extended this work and investigated the roles of Rho signaling in primary human blood monocytes migrating in chemotactic gradients and in various settings. Monocyte velocity, but not chemotactic navigation, was modestly dependent on Rho-ROCK-myosin II signaling on a 2D substrate or in a loose collagen type I matrix. Viewed by time-lapse epi-fluorescence microscopy, monocytes appeared to flutter rather than crawl, such that the 3D surface topology of individual cells was difficult to predict. Spinning disk confocal microscopy and 3D reconstruction revealed that cells move on planar surfaces and in a loose collagen matrix using prominent, curved planar protrusions, which are rapidly remodeled and reoriented, as well as resorbed. In a dense collagen type I matrix, there is insufficient space for this mode and cells adopt a highly Rho-dependent, lobular mode of motility. Thus, in addition to its role in tail retraction on 2D surfaces, Rho is critical for movement in confined spaces, but is largely redundant for motility and chemotaxis in loose matrices.
Y1  - 2016
U6  - https://doi.org/10.1038/srep25016
SN  - 2045-2322
VL  - 6
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Bornhorst, Julia
A1  - Ebert, Franziska
A1  - Meyer, Sören
A1  - Ziemann, Vanessa
A1  - Xiong, Chan
A1  - Guttenberger, Nikolaus
A1  - Raab, Andrea
A1  - Baesler, Jessica
A1  - Aschner, Michael
A1  - Feldmann, Jörg
A1  - Francesconi, Kevin
A1  - Raber, Georg
A1  - Schwerdtle, Tanja
T1  - Toxicity of three types of arsenolipids
BT  - species-specific effects in Caenorhabditis elegans
JF  - Metallomics
N2  - Although fish and seafood are well known for their nutritional benefits, they contain contaminants that might affect human health. Organic lipid-soluble arsenic species, so called arsenolipids, belong to the emerging contaminants in these food items; their toxicity has yet to be systematically studied. Here, we apply the in vivo model Caenorhabditis elegans to assess the effects of two arsenic-containing hydrocarbons (AsHC), a saturated arsenic-containing fatty acid (AsFA), and an arsenic-containing triacylglyceride (AsTAG) in a whole organism. Although all arsenolipids were highly bioavailable in Caenorhabditis elegans, only the AsHCs were substantially metabolized to thioxylated or shortened metabolic products and induced significant toxicity, affecting both survival and development. Furthermore, the AsHCs were several fold more potent as compared to the toxic reference arsenite. This study clearly indicates the need for a full hazard identification of subclasses of arsenolipids to assess whether they pose a risk to human health.
Y1  - 2020
U6  - https://doi.org/https://doi.org/10.1039/d0mt00039f
SN  - 1756-591X
SN  - 1756-5901
VL  - 12
IS  - 5
SP  - 794
EP  - 798
PB  - Oxford University Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Başaran, Nurşen
A1  - Duydu, Yalçın
A1  - Üstündağ, Aylin
A1  - Taner, Gökçe
A1  - Aydin Dilsiz, Sevtap
A1  - Anlar, Hatice Gül
A1  - Yalçin, Can Özgür
A1  - Bacanli, Merve
A1  - Golka, Klaus
A1  - Schwerdtle, Tanja
A1  - Bolt, Hermann M.
T1  - Environmental boron exposure does not induce DNA damage in lymphocytes and buccal cells of females DNA damage in lymphocytes and buccal cells of boron exposed females
JF  - Journal of trace elements in medicine and biology
N2  - Boron (B) compounds are essential for plants and animals and beneficial for humans in nutritional amounts. I animals and humans increasing evidence have shown beneficial effects on B compounds on nutrition and on antioxidant status. The genotoxic effects of environmental B exposure in women living in boron-rich and boronpoor areas was examined in this study. For this purpose, the DNA damage in the lymphocytes and buccal cells of females were assessed by Comet and micronucleus (MN) assays respectively. No significant difference was observed in the DNA damage of the lymphocytes of B exposed groups of female volunteers in Comet assay. Even buccal micronucleus (MN) frequency observed in the high exposure group was significantly lower than the low exposure group (p < 0.05). The results of this study came to the same conclusions of the previous studies that boron does not induce DNA damage even under extreme exposure conditions.
KW  - Boric acid
KW  - Boron exposure
KW  - DNA damage
Y1  - 2019
U6  - https://doi.org/10.1016/j.jtemb.2019.03.004
SN  - 0946-672X
VL  - 53
SP  - 150
EP  - 153
PB  - Elsevier B.V.
CY  - München
ER  - 
TY  - JOUR
A1  - Basaran, Nursen
A1  - Duydu, Yalcin
A1  - Ustundag, Aylin
A1  - Taner, Gokce
A1  - Aydin, Sevtap
A1  - Anlar, Hatice Gul
A1  - Yalcin, Can Özgür
A1  - Bacanli, Merve
A1  - Aydos, Kaan
A1  - Atabekoglu, Cem Somer
A1  - Golka, Klaus
A1  - Ickstadt, Katja
A1  - Schwerdtle, Tanja
A1  - Werner, Matthias
A1  - Meyer, Sören
A1  - Bolt, Hermann M.
T1  - Evaluation of the DNA damage in lymphocytes, sperm and buccal cells of workers under environmental and occupational boron exposure conditions
JF  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
N2  - Industrial production and use of boron compounds have increased during the last decades, especially for the manufacture of borosilicate glass, fiberglass, metal alloys and flame retardants. This study was conducted in two districts of Balikesir; Bandirma and Bigadic, which geographically belong to the Marmara Region of Turkey. Bandirma is the production and exportation zone for the produced boric acid and some borates and Bigadic has the largest B deposits in Turkey. 102 male workers who were occupationally exposed to boron from Bandirma and 110 workers who were occupationally and environmentally exposed to boron from Bigadic participated to our study. In this study the DNA damage in the sperm, blood and buccal cells of 212 males was evaluated by comet and micronucleus assays. No significant increase in the DNA damage in blood, sperm and buccal cells was observed in the residents exposed to boron both occupationally and environmentally (p = 0.861) for Comet test in the sperm samples, p = 0.116 for Comet test in the lymphocyte samples, p = 0.042 for micronucleus (MN) test, p = 0.955 for binucleated cells (BN), p = 1.486 for condensed chromatin (CC), p = 0.455 for karyorrhectic cells (KHC), p = 0.541 for karyolitic cells (KLY), p = 1.057 for pyknotic cells (PHC), p = 0.331 for nuclear bud (NBUD)). No correlations were seen between blood boron levels and tail intensity values of the sperm samples, lymphocyte samples, frequencies of MN, BN, KHC, KYL, PHC and NBUD. The results of this study came to the same conclusions of the previous studies that boron does not induce DNA damage even under extreme exposure conditions.
KW  - Boric acid
KW  - Boron exposure
KW  - DNA damage
KW  - Comet assay
Y1  - 2019
U6  - https://doi.org/10.1016/j.mrgentox.2018.12.013
SN  - 1383-5718
SN  - 1879-3592
VL  - 843
SP  - 33
EP  - 39
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Baesler, Jessica
A1  - Michaelis, Vivien
A1  - Stiboller, Michael
A1  - Haase, Hajo
A1  - Aschner, Michael
A1  - Schwerdtle, Tanja
A1  - Sturzenbaum, Stephen R.
A1  - Bornhorst, Julia
T1  - Nutritive manganese and zinc overdosing in aging c. elegans result in a metallothionein-mediated alteration in metal homeostasis
JF  - Molecular Nutrition and Food Research
N2  - Manganese (Mn) and zinc (Zn) are not only essential trace elements, but also potential exogenous risk factors for various diseases. Since the disturbed homeostasis of single metals can result in detrimental health effects, concerns have emerged regarding the consequences of excessive exposures to multiple metals, either via nutritional supplementation or parenteral nutrition. This study focuses on Mn-Zn-interactions in the nematode Caenorhabditis elegans (C. elegans) model, taking into account aspects related to aging and age-dependent neurodegeneration.
KW  - aging
KW  - C. elegans
KW  - homeostasis
KW  - manganese
KW  - zinc
Y1  - 2021
U6  - https://doi.org/10.1002/mnfr.202001176
SN  - 1613-4133
SN  - 1613-4125
VL  - 65
IS  - 8
SP  - 1
EP  - 11
PB  - Wiley-VCH GmbH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Baesler, Jessica
A1  - Kopp, Johannes Florian
A1  - Pohl, Gabriele
A1  - Aschner, Michael
A1  - Haase, Hajo
A1  - Schwerdtle, Tanja
A1  - Bornhorst, Julia
T1  - Zn homeostasis in genetic models of Parkinson’s disease in Caenorhabditis elegans
JF  - Journal of Trace Elements in Medicine and Biology
N2  - While the underlying mechanisms of Parkinson’s disease (PD) are still insufficiently studied, a complex interaction between genetic and environmental factors is emphasized. Nevertheless, the role of the essential trace element zinc (Zn) in this regard remains controversial. In this study we altered Zn balance within PD models of the versatile model organism Caenorhabditis elegans (C. elegans) in order to examine whether a genetic predisposition in selected genes with relevance for PD affects Zn homeostasis. Protein-bound and labile Zn species act in various areas, such as enzymatic catalysis, protein stabilization pathways and cell signaling. Therefore, total Zn and labile Zn were quantitatively determined in living nematodes as individual biomarkers of Zn uptake and bioavailability with inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) or a multi-well method using the fluorescent probe ZinPyr-1. Young and middle-aged deletion mutants of catp-6 and pdr-1, which are orthologues of mammalian ATP13A2 (PARK9) and parkin (PARK2), showed altered Zn homeostasis following Zn exposure compared to wildtype worms. Furthermore, age-specific differences in Zn uptake were observed in wildtype worms for total as well as labile Zn species. These data emphasize the importance of differentiation between Zn species as meaningful biomarkers of Zn uptake as well as the need for further studies investigating the role of dysregulated Zn homeostasis in the etiology of PD.
KW  - Caenorhabditis elegans
KW  - Zinc
KW  - Zinc homeostasis
KW  - Parkinson disease
KW  - Labile zinc
Y1  - 2019
U6  - https://doi.org/10.1016/j.jtemb.2019.05.005
VL  - 55
SP  - 44
EP  - 49
PB  - Elsevier
CY  - München
ER  - 
TY  - JOUR
A1  - Baesler, Jessica
A1  - Kopp, Johannes F.
A1  - Pohl, Gabriele
A1  - Aschner, Michael
A1  - Haase, Hajo
A1  - Schwerdtle, Tanja
A1  - Bornhorst, Julia
T1  - Zn homeostasis in genetic models of Parkinson’s disease in Caenorhabditis elegans
JF  - Journal of trace elements in medicine and biology
KW  - Caenorhabditis elegans
KW  - Zinc
KW  - Zinc homeostasis
KW  - Parkinson disease
KW  - Labile zinc
Y1  - 2019
U6  - https://doi.org/10.1016/j.jtemb.2019.05.005
SN  - 0946-672X
VL  - 55
SP  - 44
EP  - 49
PB  - Elsevier GMBH
CY  - München
ER  - 
TY  - JOUR
A1  - Alker, Wiebke
A1  - Schwerdtle, Tanja
A1  - Schomburg, Lutz
A1  - Haase, Hajo
T1  - A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum
JF  - International journal of molecular sciences
N2  - Zinc is an essential trace element, making it crucial to have a reliable biomarker for evaluating an individual’s zinc status. The total serum zinc concentration, which is presently the most commonly used biomarker, is not ideal for this purpose, but a superior alternative is still missing. The free zinc concentration, which describes the fraction of zinc that is only loosely bound and easily exchangeable, has been proposed for this purpose, as it reflects the highly bioavailable part of serum zinc. This report presents a fluorescence-based method for determining the free zinc concentration in human serum samples, using the fluorescent probe Zinpyr-1. The assay has been applied on 154 commercially obtained human serum samples. Measured free zinc concentrations ranged from 0.09 to 0.42 nM with a mean of 0.22 ± 0.05 nM. It did not correlate with age or the total serum concentrations of zinc, manganese, iron or selenium. A negative correlation between the concentration of free zinc and total copper has been seen for sera from females. In addition, the free zinc concentration in sera from females (0.21 ± 0.05 nM) was significantly lower than in males (0.23 ± 0.06 nM). The assay uses a sample volume of less than 10 µL, is rapid and cost-effective and allows us to address questions regarding factors influencing the free serum zinc concentration, its connection with the body’s zinc status, and its suitability as a future biomarker for an individual’s zinc status.
KW  - zinc
KW  - free zinc
KW  - serum
KW  - biomarker
KW  - fluorescent probe
KW  - Zinypr-1
Y1  - 2019
U6  - https://doi.org/10.3390/ijms20164006
SN  - 1661-6596
SN  - 1422-0067
VL  - 20
IS  - 16
PB  - MDPI
CY  - Basel
ER  -