TY  - JOUR
A1  - Rieck, Christoph Paul Kurt
A1  - Geiger, Daniel
A1  - Munkert, Jennifer
A1  - Messerschmidt, Katrin
A1  - Petersen, Jan
A1  - Strasser, Juliane
A1  - Meitinger, Nadine
A1  - Kreis, Wolfgang
T1  - Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae
JF  - Microbiologyopen
N2  - A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5‐isomerase gene from Comamonas testosteronii, (c) a mutated steroid‐5β‐reductase gene from Arabidopsis thaliana, and (d) a steroid 21‐hydroxylase gene from Mus musculus. A second plasmid bearing an ADR/ADX fusion gene from Bos taurus was also constructed. A Saccharomyces cerevisiae strain bearing these two plasmids was generated. This strain, termed “CARD II yeast”, was capable of producing 5β‐pregnane‐3β,21‐diol‐20‐one, a central intermediate in 5β‐cardenolide biosynthesis, starting from pregnenolone which was added to the culture medium. Using this approach, five consecutive steps in cardenolide biosynthesis were realized in baker's yeast.
Y1  - 2019
U6  - https://doi.org/10.1002/mbo3.925
SN  - 2045-8827
VL  - 8
IS  - 12
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Wang, Wei-shi
A1  - Oswald, Sascha
A1  - Gräff, Thomas
A1  - Lensing, Hermann Josef
A1  - Liu, Tie
A1  - Strasser, Daniel
A1  - Munz, Matthias
T1  - Impact of river reconstruction on groundwater flow during bank filtration assessed by transient three-dimensional modelling of flow and heat transport
JF  - Hydrogeology journal : official journal of the International Association of Hydrogeologists
N2  - Bank filtration (BF) is an established indirect water-treatment technology. The quality of water gained via BF depends on the subsurface capture zone, the mixing ratio (river water versus ambient groundwater), spatial and temporal distribution of subsurface travel times, and subsurface temperature patterns. Surface-water infiltration into the adjacent aquifer is determined by the local hydraulic gradient and riverbed permeability, which could be altered by natural clogging, scouring and artificial decolmation processes. The seasonal behaviour of a BF system in Germany, and its development during and about 6 months after decolmation (canal reconstruction), was observed with a long-term monitoring programme. To quantify the spatial and temporal variation in the BF system, a transient flow and heat transport model was implemented and two model scenarios, 'with' and 'without' canal reconstruction, were generated. Overall, the simulated water heads and temperatures matched those observed. Increased hydraulic connection between the canal and aquifer caused by the canal reconstruction led to an increase of similar to 23% in the already high share of BF water abstracted by the nearby waterworks. Subsurface travel-time distribution substantially shifted towards shorter travel times. Flow paths with travel times <200 days increased by similar to 10% and those with <300 days by 15%. Generally, the periodic temperature signal, and the summer and winter temperature extrema, increased and penetrated deeper into the aquifer. The joint hydrological and thermal effects caused by the canal reconstruction might increase the potential of biodegradable compounds to further penetrate into the aquifer, also by potentially affecting the redox zonation in the aquifer.
KW  - bank filtration
KW  - groundwater
KW  - surface-water relations
KW  - groundwater modelling
Y1  - 2019
U6  - https://doi.org/10.1007/s10040-019-02063-3
SN  - 1431-2174
SN  - 1435-0157
VL  - 28
IS  - 2
SP  - 723
EP  - 743
PB  - Springer
CY  - Berlin ; Heidelberg [u.a.]
ER  - 
TY  - GEN
A1  - Wang, Wei-shi
A1  - Oswald, Sascha
A1  - Gräff, Thomas
A1  - Lensing, Hermann-Josef
A1  - Liu, Tie
A1  - Strasser, Daniel
A1  - Munz, Matthias
T1  - Correction: Impact of river reconstruction on groundwater flow during bank filtration assessed by transient three-dimensional modelling of flow and heat transport. - Hydrogeology Journal. - Berlin: Springer. -  28 (2020) , S. 723. - https://doi.org/10.1007/s10040-019-02063-3
T2  - Hydrogeology journal : official journal of the International Association of Hydrogeologists
T2  - Erratum: Impact de la reconstruction d’une rivière sur l’écoulement des eaux souterraines via la filtration sur berge évalué par un modèle tridimensionnel en régime transitoire de l’écoulement et du transport de chaleur. - Berlin: Springer. -  28 (2020) , S. 723. - https://doi.org/10.1007/s10040-019-02063-3
T2  - Erratum: Impacto de la restauración de un río en el flujo de agua subterránea durante la filtración en las márgenes, evaluado mediante la modelización tridimensional transitoria del flujo y el transporte de calor. - Berlin: Springer. -  28 (2020) , S. 723. - https://doi.org/10.1007/s10040-019-02063-3
Y1  - 2020
U6  - https://doi.org/10.1007/s10040-020-02221-y
SN  - 1431-2174
SN  - 1435-0157
VL  - 28
IS  - 7
SP  - 2633
EP  - 2634
PB  - Springer
CY  - Berlin ; Heidelberg ; New York, NY
ER  -