TY - JOUR A1 - Fritz, Michael Andre A1 - Rosa, Stefanie A1 - Sicard, Adrien T1 - Mechanisms Underlying the Environmentally Induced Plasticity of Leaf Morphology JF - Frontiers in genetics N2 - The primary function of leaves is to provide an interface between plants and their environment for gas exchange, light exposure and thermoregulation. Leaves have, therefore a central contribution to plant fitness by allowing an efficient absorption of sunlight energy through photosynthesis to ensure an optimal growth. Their final geometry will result from a balance between the need to maximize energy uptake while minimizing the damage caused by environmental stresses. This intimate relationship between leaf and its surroundings has led to an enormous diversification in leaf forms. Leaf shape varies between species, populations, individuals or even within identical genotypes when those are subjected to different environmental conditions. For instance, the extent of leaf margin dissection has, for long, been found to inversely correlate with the mean annual temperature, such that Paleobotanists have used models based on leaf shape to predict the paleoclimate from fossil flora. Leaf growth is not only dependent on temperature but is also regulated by many other environmental factors such as light quality and intensity or ambient humidity. This raises the question of how the different signals can be integrated at the molecular level and converted into clear developmental decisions. Several recent studies have started to shed the light on the molecular mechanisms that connect the environmental sensing with organ-growth and patterning. In this review, we discuss the current knowledge on the influence of different environmental signals on leaf size and shape, their integration as well as their importance for plant adaptation. KW - plants KW - leaf morphology KW - environment KW - developmental plasticity KW - gene regulatory networks KW - sensory system KW - gene responsiveness Y1 - 2018 U6 - https://doi.org/10.3389/fgene.2018.00478 SN - 1664-8021 VL - 9 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Berry, Scott A1 - Rosa, Stefanie A1 - Howard, Martin A1 - Buhler, Marc A1 - Dean, Caroline T1 - Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression JF - Genes & Development N2 - Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNAbinding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression. KW - chromatin KW - epigenetics KW - plant biology KW - Polycomb KW - RNA Y1 - 2017 U6 - https://doi.org/10.1101/gad.305227.117 SN - 0890-9369 SN - 1549-5477 VL - 31 SP - 2115 EP - 2120 PB - Cold Spring Harbor Laboratory Press CY - Cold Spring Harbor, NY ER - TY - JOUR A1 - Mendling, Jan A1 - Weber, Ingo A1 - van der Aalst, Wil A1 - Brocke, Jan Vom A1 - Cabanillas, Cristina A1 - Daniel, Florian A1 - Debois, Soren A1 - Di Ciccio, Claudio A1 - Dumas, Marlon A1 - Dustdar, Schahram A1 - Gal, Avigdor A1 - Garcia-Banuelos, Luciano A1 - Governatori, Guido A1 - Hull, Richard A1 - La Rosa, Marcello A1 - Leopold, Henrik A1 - Leymann, Frank A1 - Recker, Jan A1 - Reichert, Manfred A1 - Reijers, Hajo A. A1 - Rinderle-Ma, Stefanie A1 - Solti, Andreas A1 - Rosemann, Michael A1 - Schulte, Stefan A1 - Singh, Munindar P. A1 - Slaats, Tijs A1 - Staples, Mark A1 - Weber, Barbara A1 - Weidlich, Matthias A1 - Weske, Mathias A1 - Xu, Xiwei A1 - Zhu, Liming T1 - Blockchains for Business Process Management BT - Challenges and Opportunities JF - ACM Transactions on Management Information Systems N2 - Blockchain technology offers a sizable promise to rethink the way interorganizational business processes are managed because of its potential to realize execution without a central party serving as a single point of trust (and failure). To stimulate research on this promise and the limits thereof, in this article, we outline the challenges and opportunities of blockchain for business process management (BPM). We first reflect how blockchains could be used in the context of the established BPM lifecycle and second how they might become relevant beyond. We conclude our discourse with a summary of seven research directions for investigating the application of blockchain technology in the context of BPM. KW - Blockchain KW - business process management KW - research challenges Y1 - 2018 U6 - https://doi.org/10.1145/3183367 SN - 2158-656X SN - 2158-6578 VL - 9 IS - 1 SP - 1 EP - 16 PB - Association for Computing Machinery CY - New York ER - TY - JOUR A1 - Ietswaart, Robert A1 - Rosa, Stefanie A1 - Wu, Zhe A1 - Dean, Caroline A1 - Howard, Martin T1 - Cell-Size-Dependent Transcription of FLC and Its Antisense Long Non-coding RNA COOLAIR Explain Cell-to-Cell Expression Variation JF - Cell systems N2 - Single-cell quantification of transcription kinetics and variability promotes a mechanistic understanding of gene regulation. Here, using single-molecule RNA fluorescence in situ hybridization and mathematical modeling, we dissect cellular RNA dynamics for Arabidopsis FLOWERING LOCUS C (FLC). FLC expression quantitatively determines flowering time and is regulated by antisense (COOLAIR) transcription. In cells without observable COOLAIR expression, we quantify FLC transcription initiation, elongation, intron processing, and lariat degradation, as well as mRNA release from the locus and degradation. In these heterogeneously sized cells, FLC mRNA number increases linearly with cell size, resulting in a large cell-to-cell variability in transcript level. This variation is accounted for by cell-sizedependent, Poissonian FLC mRNA production, but not by large transcriptional bursts. In COOLAIRexpressing cells, however, antisense transcription increases with cell size and contributes to FLC transcription decreasing with cell size. Our analysis therefore reveals an unexpected role for antisense transcription in modulating the scaling of transcription with cell size. Y1 - 2017 U6 - https://doi.org/10.1016/j.cels.2017.05.010 SN - 2405-4712 SN - 2405-4720 VL - 4 SP - 622 EP - 635 PB - Cell Press CY - Cambridge ER - TY - JOUR A1 - Duncan, Susan A1 - Rosa, Stefanie Nunes T1 - Gaining insight into plant gene transcription using smFISH JF - Transcription N2 - Single molecule RNA fluorescent in situ hybridization (smFISH) enables gene transcription to be assessed at the cellular level. In this point of view article, we describe our recent smFISH research in the model plant Arabidopsis thaliana and discuss how this technique could further knowledge of plant gene transcription in the future. KW - Arabidopsis KW - lncRNA KW - mRNA Quantification KW - RNA Imaging KW - smFISH Y1 - 2017 U6 - https://doi.org/10.1080/21541264.2017.1372043 SN - 2154-1264 SN - 2154-1272 VL - 9 IS - 3 SP - 166 EP - 170 PB - Taylor & Francis Group CY - Philadelphia ER -