TY  - JOUR
A1  - Dunsing, Valentin
A1  - Irmscher, Tobias
A1  - Barbirz, Stefanie
A1  - Chiantia, Salvatore
T1  - Purely Polysaccharide-Based Biofilm Matrix Provides Size-Selective Diffusion Barriers for Nanoparticles and Bacteriophages
JF  - Biomacromolecules : an interdisciplinary journal focused at the interface of polymer science and the biological sciences
N2  - Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle. However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization. To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii. Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules. Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion. We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.
Y1  - 2019
U6  - https://doi.org/10.1021/acs.biomac.9b00938
SN  - 1525-7797
SN  - 1526-4602
VL  - 20
IS  - 10
SP  - 3842
EP  - 3854
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - GEN
A1  - Dunsing, Valentin
A1  - Irmscher, Tobias
A1  - Barbirz, Stefanie
A1  - Chiantia, Salvatore
T1  - Microviscosity of bacterial biofilm matrix characterized by fluorescence correlation spectroscopy and single particle tracking
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2019
U6  - https://doi.org/https://doi.org/10.1007/s00249-019-01373-4
SN  - 0175-7571
SN  - 1432-1017
VL  - 48
SP  - S115
EP  - S115
PB  - Springer
CY  - New York
ER  -