TY - JOUR A1 - Risse, Sarah A1 - Seelig, Stefan T1 - Stable preview difficulty effects in reading with an improved variant of the boundary paradigm JF - The quarterly journal of experimental psychology N2 - Using gaze-contingent display changes in the boundary paradigm during sentence reading, it has recently been shown that parafoveal word-processing difficulties affect fixations on words to the right of the boundary. Current interpretations of this post-boundary preview difficulty effect range from delayed parafoveal-on-foveal effects in parallel word-processing models to forced fixations in serial word-processing models. However, these findings are based on an experimental design that, while allowing to isolate preview difficulty effects, might have established a bias with respect to asymmetries in parafoveal preview benefit for high-frequent and low-frequent target words. Here, we present a revision of this paradigm varying the preview’s lexical frequency and keeping the target word constant. We found substantial effects of the preview difficulty in fixation durations after the boundary confirming that preview processing affects the oculomotor decisions not only via trans-saccadic integration of preview and target word information. An additional time-course analysis showed that the preview difficulty effect was significant across the full fixation duration distribution on the target word without any evidence on the pretarget word before the boundary. We discuss implications of the accumulating evidence of post-boundary preview difficulty effects for models of eye movement control during reading. KW - Eye tracking KW - reading KW - parafoveal preview benefit KW - delayed parafoveal-on-foveal effects KW - forced fixations Y1 - 2019 U6 - https://doi.org/10.1177/1747021818819990 SN - 1747-0218 SN - 1747-0226 VL - 72 IS - 7 SP - 1632 EP - 1645 PB - Sage Publ. CY - London ER - TY - GEN A1 - Seelig, Stefan A1 - Risse, Sarah A1 - Engbert, Ralf T1 - Predictive modeling of parafoveal information processing during reading T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Skilled reading requires information processing of the fixated and the not-yet-fixated words to generate precise control of gaze. Over the last 30 years, experimental research provided evidence that word processing is distributed across the perceptual span, which permits recognition of the fixated (foveal) word as well as preview of parafoveal words to the right of fixation. However, theoretical models have been unable to differentiate the specific influences of foveal and parafoveal information on saccade control. Here we show how parafoveal word difficulty modulates spatial and temporal control of gaze in a computational model to reproduce experimental results. In a fully Bayesian framework, we estimated model parameters for different models of parafoveal processing and carried out large-scale predictive simulations and model comparisons for a gaze-contingent reading experiment. We conclude that mathematical modeling of data from gaze-contingent experiments permits the precise identification of pathways from parafoveal information processing to gaze control, uncovering potential mechanisms underlying the parafoveal contribution to eye-movement control. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 728 Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-526665 SN - 1866-8364 ER - TY - JOUR A1 - Seelig, Stefan A1 - Risse, Sarah A1 - Engbert, Ralf T1 - Predictive modeling of parafoveal information processing during reading JF - Scientific Reports N2 - Skilled reading requires information processing of the fixated and the not-yet-fixated words to generate precise control of gaze. Over the last 30 years, experimental research provided evidence that word processing is distributed across the perceptual span, which permits recognition of the fixated (foveal) word as well as preview of parafoveal words to the right of fixation. However, theoretical models have been unable to differentiate the specific influences of foveal and parafoveal information on saccade control. Here we show how parafoveal word difficulty modulates spatial and temporal control of gaze in a computational model to reproduce experimental results. In a fully Bayesian framework, we estimated model parameters for different models of parafoveal processing and carried out large-scale predictive simulations and model comparisons for a gaze-contingent reading experiment. We conclude that mathematical modeling of data from gaze-contingent experiments permits the precise identification of pathways from parafoveal information processing to gaze control, uncovering potential mechanisms underlying the parafoveal contribution to eye-movement control. Y1 - 2021 SN - 2045-2322 VL - 11 PB - Springer Nature CY - Berlin ER - TY - JOUR A1 - Seelig, Stefan A1 - Risse, Sarah A1 - Engbert, Ralf T1 - Predictive modeling of parafoveal information processing during reading JF - Scientific reports N2 - Skilled reading requires information processing of the fixated and the not-yet-fixated words to generate precise control of gaze. Over the last 30 years, experimental research provided evidence that word processing is distributed across the perceptual span, which permits recognition of the fixated (foveal) word as well as preview of parafoveal words to the right of fixation. However, theoretical models have been unable to differentiate the specific influences of foveal and parafoveal information on saccade control. Here we show how parafoveal word difficulty modulates spatial and temporal control of gaze in a computational model to reproduce experimental results. In a fully Bayesian framework, we estimated model parameters for different models of parafoveal processing and carried out large-scale predictive simulations and model comparisons for a gaze-contingent reading experiment. We conclude that mathematical modeling of data from gaze-contingent experiments permits the precise identification of pathways from parafoveal information processing to gaze control, uncovering potential mechanisms underlying the parafoveal contribution to eye-movement control. Y1 - 2021 U6 - https://doi.org/10.1038/s41598-021-92140-z SN - 2045-2322 VL - 11 IS - 1 PB - Nature Portfolio CY - Berlin ER - TY - JOUR A1 - Mc Carthy, Denis N. A1 - Risse, Stefan A1 - Katekomol, Phisan A1 - Kofod, Guggi T1 - The effect of dispersion on the increased relative permittivity of TiO2/SEBS composites N2 - Polymer composites are currently suggested for use as improved dielectric materials in many applications. Here, the effect of particle size and dispersion on the electrical properties of composites of rutile TiO2 and poly(styrene- ethylene-butadiene-styrene) (SEBS) are investigated. Both 15 and 300 nm particles are mixed with SEBS, with amounts of sorbitan monopalmitate surfactant from 0 to 3.3 vol%, and their dielectric and mechanical properties are measured. Composites with the 300 nm TiO2 particles result in increases of 170% in relative permittivity over the pure polymer, far above those predicted by standard theories, such as Bruggeman (140%) and Yamada (114%), and improving dispersion with surfactant has little effect. The composites with 15 nm particles showed surprisingly large relative permittivity increases (350%), but improving the dispersion by the addition of any surfactant causes the relative permittivity to decrease to 240% of the pure polymer value. We suggest that the increase is due to the formation of a highly conductive layer in the polymer around the TiO2 particles. Y1 - 2009 UR - http://iopscience.iop.org/0022-3727/ U6 - https://doi.org/10.1088/0022-3727/42/14/145406 SN - 0022-3727 ER - TY - JOUR A1 - Hahn, Aaron A1 - Reschke, Stefan A1 - Leimkühler, Silke A1 - Risse, Thomas T1 - Ketoxime coupling of p-Acetylphenylalanine at neutral pH for site-directed spin labeling of human sulfite oxidase JF - The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces & biophysical chemistry N2 - Site-directed spin labeling of the unnatural amino acid p-acetylphenylalanine (p-AcPhe) using oxime based coupling chemistry is successfully applied to investigate human sulfite oxidase (hSO), a protein containing an essential cysteine residue, which impedes the use of thiol based coupling chemistry. The protein was found to be sensitive toward typical reaction conditions of oxime coupling, namely, acidic reaction conditions and elevated temperatures. Thus, coupling at neutral pH and room temperature is mandatory. Three catalysts described in the literature to accelerate the reaction rate have been tested. Best spin labeling efficiencies were observed for p-methoxyaniline, while the other catalysts described in the literature to have even better performance for oxime coupling at neutral pH were substantially less active or led to precipitation of the protein. A clear correlation of spin labeling efficiency with the local environment of the residue is found, shedding some light on the importance of the sterically demanding reaction complex between p-AcPhe, the aniline catalyst, and the spin label for the reaction rate. The analysis of the line shape has shown that its interpretation in terms of local environment is more challenging as compared to the well-established spin labels based on cysteine chemistry. To this end the results presented here indicate that the larger steric demand of the spin labeled p-AcPhe can induce structural effects instead of reporting on them. Y1 - 2014 U6 - https://doi.org/10.1021/jp503471j SN - 1520-6106 VL - 118 IS - 25 SP - 7077 EP - 7084 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Hahn, Aaron A1 - Engelhard, Christopher A1 - Reschke, Stefan A1 - Teutloff, Christian A1 - Bittl, Robert A1 - Leimkühler, Silke A1 - Risse, Thomas T1 - Structural Insights into the Incorporation of the Mo Cofactor into Sulfite Oxidase from Site-Directed Spin Labeling JF - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition N2 - Mononuclear molybdoenzymes catalyze a broad range of redox reactions and are highly conserved in all kingdoms of life. This study addresses the question of how the Mo cofactor (Moco) is incorporated into the apo form of human sulfite oxidase (hSO) by using site-directed spin labeling to determine intramolecular distances in the nanometer range. Comparative measurements of the holo and apo forms of hSO enabled the localization of the corresponding structural changes, which are localized to a short loop (residues 263-273) of the Moco-containing domain. A flap-like movement of the loop provides access to the Moco binding-pocket in the apo form of the protein and explains the earlier studies on the in vitro reconstitution of apo-hSO with Moco. Remarkably, the loop motif can be found in a variety of structurally similar molybdoenzymes among various organisms, thus suggesting a common mechanism of Moco incorporation. KW - biocatalysis KW - cofactors KW - enzymes KW - EPR spectroscopy KW - protein structures Y1 - 2015 U6 - https://doi.org/10.1002/anie.201504772 SN - 1433-7851 SN - 1521-3773 VL - 54 IS - 40 SP - 11865 EP - 11869 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Seelig, Stefan A. A1 - Rabe, Maximilian Michael A1 - Malem-Shinitski, Noa A1 - Risse, Sarah A1 - Reich, Sebastian A1 - Engbert, Ralf T1 - Bayesian parameter estimation for the SWIFT model of eye-movement control during reading JF - Journal of mathematical psychology N2 - Process-oriented theories of cognition must be evaluated against time-ordered observations. Here we present a representative example for data assimilation of the SWIFT model, a dynamical model of the control of fixation positions and fixation durations during natural reading of single sentences. First, we develop and test an approximate likelihood function of the model, which is a combination of a spatial, pseudo-marginal likelihood and a temporal likelihood obtained by probability density approximation Second, we implement a Bayesian approach to parameter inference using an adaptive Markov chain Monte Carlo procedure. Our results indicate that model parameters can be estimated reliably for individual subjects. We conclude that approximative Bayesian inference represents a considerable step forward for computational models of eye-movement control, where modeling of individual data on the basis of process-based dynamic models has not been possible so far. KW - dynamical models KW - reading KW - eye movements KW - saccades KW - likelihood function KW - Bayesian inference KW - MCMC KW - interindividual differences Y1 - 2020 U6 - https://doi.org/10.1016/j.jmp.2019.102313 SN - 0022-2496 SN - 1096-0880 VL - 95 PB - Elsevier CY - San Diego ER -