TY - JOUR A1 - Schlör, Anja A1 - Hirschberg, Stefan A1 - Ben Amor, Ghada A1 - Meister, Toni Luise A1 - Arora, Prerna A1 - Pöhlmann, Stefan A1 - Hoffmann, Markus A1 - Pfänder, Stephanie A1 - Eddin, Omar Kamal A1 - Kamhieh-Milz, Julian A1 - Hanack, Katja T1 - SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications JF - Frontiers in Immunology N2 - Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications. Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity. Methods: Five antibody candidates were selected out of a naïve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron). Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50). Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy. KW - camelid heavy-chain-only antibodies KW - single domain antibodies KW - nanobodies KW - SARS-CoV-2 KW - neutralization KW - Omicron Y1 - 2022 U6 - https://doi.org/10.3389/fimmu.2022.930975 SN - 1664-3224 SP - 1 EP - 14 PB - Frontiers Media SA CY - Lausanne, Schweiz ER - TY - GEN A1 - Schlör, Anja A1 - Hirschberg, Stefan A1 - Ben Amor, Ghada A1 - Meister, Toni Luise A1 - Arora, Prerna A1 - Pöhlmann, Stefan A1 - Hoffmann, Markus A1 - Pfänder, Stephanie A1 - Eddin, Omar Kamal A1 - Kamhieh-Milz, Julian A1 - Hanack, Katja T1 - SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications. Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity. Methods: Five antibody candidates were selected out of a naïve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron). Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50). Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1280 KW - camelid heavy-chain-only antibodies KW - single domain antibodies KW - nanobodies KW - SARS-CoV-2 KW - neutralization KW - Omicron Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-570124 SN - 1866-8372 IS - 1280 ER - TY - JOUR A1 - Wilson, Peter A1 - Huhtamies, Mikko A1 - Huck, Stephan A1 - Gahlen, Gundula A1 - Herrmann, Michael A1 - Reimer, Torsten F. A1 - Winnige, Norbert A1 - Pöhlmann, Markus A1 - Pauser, Josef A1 - Fuchs, Antje A1 - Möbius, Sascha A1 - Heil, Wolfgang A1 - Jacobs, Rainer A1 - Kaiser, Michael A1 - Kroll, Stefan A1 - Blank, Ralf A1 - Gründel, Olaf A1 - Schlürmann, Jan A1 - Geffarth, Renko A1 - Bavendamm, Gundula T1 - Militär und Gesellschaft in der Frühen Neuzeit N2 - Aus dem Inhalt dieser Ausgabe: BEITRÄGE: Peter H. Wilson: British and american perspectives on early modern warfare Mikko Huhtamies: Kriegswesen und Gesellschaft in der frühen Neuzeit in der finnisch-schwedischen Geschichtsforschung INTERNET: Stephan Huck: Nutzungsmöglichkeiten des Internets Gundula Gahlen , Michael Herrmann, Torsten F. Reimer und Norbert Winnige: Militärgeschichte der Frühen Neuzeit im Internet Die Online-Angebote des AMG und sfn Markus Pöhlmann: Die Internetpräsenz des Arbeitskreises Militärgeschichte e.V. Josef Pauser: Das Online-Angebot des Arbeitskreises .Policey/Polizei im vormodernen Europa. Torsten F. Reimer: Eine Studie in Kriegführung. Eine Vorstellung des H-War Military History Network PROJEKTE: Antje Fuchs: Ein neuer Konfessionskrieg? Erfahrungen von Krieg und Okkupation im Kurfürstentum Hannover und benachbarten geistlichen Fürstentümern zur Zeit des Siebenjährigen Krieges 1756-1763 Sascha Möbius: Ergebnisse der Magisterarbeit .Psychologische Aspekte friederizianischer Taktik im Siebenjährigen Krieg. Wolfgang Heil: Die Gemeinen Soldaten. Das Sozialleben der militärischen Unterschicht im altpreußischen Heer und seine Stellung in der altständischen Gesellschaft. Rainer Jacobs: Militärische Dienstpflichten in der Frühen Neuzeit Michael Kaiser und Stefan Kroll: Forschungsprojekt Militär und Religiosität in der Frühen Neuzeit Ralf Blank: Die Schlosskanonen von Hohenlimburg Artilleriegeschütze mit einer wechselvollen Geschichte Gundula Gahlen und Olaf Gründel: Kataster zur Schlacht bei Wittstock von 1636 Jan Schlürmann: Die Schleswig-Holstein-Gottorfischen Auxiliairtruppen im Spanischen Erbfolgekrieg . Ergebnisse einer Untersuchung BERICHTE: Renko Geffarth: Die besetzte res publica. Zum Verhältnis von ziviler Obrigkeit und militärischer Herrschaft in besetzten Gebieten vom Spätmittelalter bis zum 18. Jahrhundert. Gundula Bavendamm: Operationsgeschichte und moderne Historiographie. Ein Widerspruch? T3 - Militär und Gesellschaft in der frühen Neuzeit - 5, Heft 2 KW - Militär / Geschichte Y1 - 2001 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-28126 SN - 1617-9722 SN - 1861-910X VL - 5 IS - 2 ER -