TY  - JOUR
A1  - Blumenstein, Oswald
A1  - Schubert, Rudolf
A1  - Bechmann, Wolfgang
A1  - Bukowsky, Heinz
A1  - Dautz, J.
A1  - Kopp, Ingo
A1  - Knösche, Rüdiger
A1  - Portmann, H. - D.
A1  - Schachtzabel, Hartmut
A1  - Schneider, I.
A1  - Tessmann, J.
T1  - Erste Ergebnisse einer Fallstudie zur Stoffdynamik in naturschutzrelevanten Böden der Döberitzer Heide
JF  - Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam
Y1  - 1997
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-2690
SN  - 1434-2375
SN  - 1611-9339
VL  - 1
SP  - 65
EP  - 77
ER  - 
TY  - JOUR
A1  - Schneider, Rudolf
A1  - Weigert, F.
A1  - Lesnyak, V.
A1  - Leubner, S.
A1  - Lorenz, T.
A1  - Behnke, Thomas
A1  - Dubavik, A.
A1  - Joswig, J. -O.
A1  - Resch-Genger, U.
A1  - Gaponik, N.
A1  - Eychmueller, A.
T1  - pH and concentration dependence of the optical properties of thiol-capped CdTe nanocrystals in water and D2O
JF  - Physical chemistry, chemical physics : a journal of European Chemical Societies
N2  - The optical properties of semiconductor nanocrystals (SC NCs) are largely controlled by their size and surface chemistry, i.e., the chemical composition and thickness of inorganic passivation shells and the chemical nature and number of surface ligands as well as the strength of their bonds to surface atoms. The latter is particularly important for CdTe NCs, which – together with alloyed CdxHg1−xTe – are the only SC NCs that can be prepared in water in high quality without the need for an additional inorganic passivation shell. Aiming at a better understanding of the role of stabilizing ligands for the control of the application-relevant fluorescence features of SC NCs, we assessed the influence of two of the most commonly used monodentate thiol ligands, thioglycolic acid (TGA) and mercaptopropionic acid (MPA), on the colloidal stability, photoluminescence (PL) quantum yield (QY), and PL decay behavior of a set of CdTe NC colloids. As an indirect measure for the strength of the coordinative bond of the ligands to SC NC surface atoms, the influence of the pH (pD) and the concentration on the PL properties of these colloids was examined in water and D2O and compared to the results from previous dilution studies with a set of thiol-capped Cd1−xHgxTe SC NCs in D2O. As a prerequisite for these studies, the number of surface ligands was determined photometrically at different steps of purification after SC NC synthesis with Ellman's test. Our results demonstrate ligand control of the pH-dependent PL of these SC NCs, with MPA-stabilized CdTe NCs being less prone to luminescence quenching than TGA-capped ones. For both types of CdTe colloids, ligand desorption is more pronounced in H2O compared to D2O, underlining also the role of hydrogen bonding and solvent molecules.
Y1  - 2016
U6  - https://doi.org/10.1039/c6cp03123d
SN  - 1463-9076
SN  - 1463-9084
VL  - 18
SP  - 19083
EP  - 19092
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Kuhne, Maren
A1  - Dippong, Martin
A1  - Flemig, Sabine
A1  - Hoffmann, Katrin
A1  - Petsch, Kristin
A1  - Schenk, Jörg A.
A1  - Kunte, Hans-Jörg
A1  - Schneider, Rudolf J.
T1  - Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA
JF  - Journal of immunological methods
N2  - A novel method that optimizes the screening for antibody-secreting hapten-specific hybridoma cells by using flow cytometry is described. Cell clones specific for five different haptens were analyzed. We selectively double stained and analyzed fixed hybridoma cells with fluorophore-labeled haptens to demonstrate the target-selectivity, and with a fluorophore-labeled anti-mouse IgG antibody to characterize the level of surface expression of membrane-bound IgGs. ELISA measurements with the supernatants of the individual hybridoma clones revealed that antibodies from those cells, which showed the highest fluorescence intensities in the flow cytometric analysis, also displayed the highest affinities for the target antigens. The fluorescence intensity of antibody-producing cells corresponded well with the produced antibodies' affinities toward their respective antigens. Immunohistochemical staining verified the successful double labeling of the cells. Our method makes it possible to perform a high-throughput screening for hybridoma cells, which have both an adequate IgG production rate and a high target affinity. (C) 2014 Elsevier B.V. All rights reserved.
KW  - Immunization
KW  - Hapten
KW  - Monoclonal antibodies
KW  - Hybridoma
KW  - Flow cytometry
KW  - ELISA
Y1  - 2014
U6  - https://doi.org/10.1016/j.jim.2014.07.004
SN  - 0022-1759
SN  - 1872-7905
VL  - 413
SP  - 45
EP  - 56
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Dippong, Martin
A1  - Carl, Peter
A1  - Lenz, Christine
A1  - Schenk, Jörg A.
A1  - Hoffmann, Katrin
A1  - Schwaar, Timm
A1  - Schneider, Rudolf J.
A1  - Kuhne, Maren
T1  - Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies
JF  - Analytical chemistry
Y1  - 2017
U6  - https://doi.org/10.1021/acs.analchem.6b04569
SN  - 0003-2700
SN  - 1520-6882
VL  - 89
SP  - 4007
EP  - 4012
PB  - American Chemical Society
CY  - Washington
ER  -