TY - JOUR A1 - Reyes, Anibal M. A1 - Vazquez, Diego S. A1 - Zeida, Ari A1 - Hugo, Martin A1 - Dolores Pineyro, M. A1 - Ines De Armas, Maria A1 - Estrin, Dario A1 - Radi, Rafael A1 - Santos, Javier A1 - Trujillo, Madia T1 - PrxQ B from Mycobacterium tuberculosis is a monomeric, thioredoxin-dependent and highly efficient fatty acid hydroperoxide reductase JF - Free radical biology and medicine : the official journal of the Oxygen Society, a constituent member of the International Society for Free Radical Research N2 - Mycobacterium tuberculosis (M. tuberculosis) is the intracellular bacterium responsible for tuberculosis disease (TD). Inside the phagosomes of activated macrophages, M. tuberculosis is exposed to cytotoxic hydroperoxides such as hydrogen peroxide, fatty acid hydroperoxides and peroxynitrite. Thus, the characterization of the bacterial antioxidant systems could facilitate novel drug developments. In this work, we characterized the product of the gene Rv1608c from M. tuberculosis, which according to sequence homology had been annotated as a putative peroxiredoxin of the peroxiredoxin Q subfamily (PrxQ B from M. tuberculosis or MtPrxQ B). The protein has been reported to be essential for M. tuberculosis growth in cholesterol-rich medium. We demonstrated the M. tuberculosis thioredoxin B/C-dependent peroxidase activity of MtPrxQ B, which acted as a two-cysteine peroxiredoxin that could function, although less efficiently, using a one-cysteine mechanism. Through steady-state and competition kinetic analysis, we proved that the net forward rate constant of MtPrxQ B reaction was 3 orders of magnitude faster for fatty acid hydroperoxides than for hydrogen peroxide (3x10(6) vs 6x10(3) M-1 s(-1), respectively), while the rate constant of peroxynitrite reduction was (0.6-1.4) x10(6) M-1 s(-1) at pH 7.4. The enzyme lacked activity towards cholesterol hydroperoxides solubilized in sodium deoxycholate. Both thioredoxin B and C rapidly reduced the oxidized form of MtPrxQ B, with rates constants of 0.5x10(6) and 1x10(6) M-1 s(-1), respectively. Our data indicated that MtPrxQ B is monomeric in solution both under reduced and oxidized states. In spite of the similar hydrodynamic behavior the reduced and oxidized forms of the protein showed important structural differences that were reflected in the protein circular dichroism spectra. KW - Mycobacterium tuberculosis KW - Peroxiredoxin KW - Thioredoxin KW - Peroxynitrite KW - Fatty acid hydroperoxides KW - Thiol-dependent peroxidase KW - Peroxidatic and resolving cysteine Y1 - 2016 U6 - https://doi.org/10.1016/j.freeradbiomed.2016.10.005 SN - 0891-5849 SN - 1873-4596 VL - 101 SP - 249 EP - 260 PB - Elsevier CY - New York ER - TY - CHAP A1 - Jacqmin, Julien A1 - Özdemir, Paker Doğu A1 - Fell Kurban, Caroline A1 - Tunç Pekkan, Zelha A1 - Koskinen, Johanna A1 - Suonpää, Maija A1 - Seng, Cheyvuth A1 - Carlon, May Kristine Jonson A1 - Gayed, John Maurice A1 - Cross, Jeffrey S. A1 - Langseth, Inger A1 - Jacobsen, Dan Yngve A1 - Haugsbakken, Halvdan A1 - Bethge, Joseph A1 - Serth, Sebastian A1 - Staubitz, Thomas A1 - Wuttke, Tobias A1 - Nordemann, Oliver A1 - Das, Partha-Pratim A1 - Meinel, Christoph A1 - Ponce, Eva A1 - Srinath, Sindhu A1 - Allegue, Laura A1 - Perach, Shai A1 - Alexandron, Giora A1 - Corti, Paola A1 - Baudo, Valeria A1 - Turró, Carlos A1 - Moura Santos, Ana A1 - Nilsson, Charlotta A1 - Maldonado-Mahauad, Jorge A1 - Valdiviezo, Javier A1 - Carvallo, Juan Pablo A1 - Samaniego-Erazo, Nicolay A1 - Poce, Antonella A1 - Re, Maria Rosaria A1 - Valente, Mara A1 - Karp Gershon, Sa’ar A1 - Ruipérez-Valiente, José A. A1 - Despujol, Ignacio A1 - Busquets, Jaime A1 - Kerr, John A1 - Lorenz, Anja A1 - Schön, Sandra A1 - Ebner, Martin A1 - Wittke, Andreas A1 - Beirne, Elaine A1 - Nic Giolla Mhichíl, Mairéad A1 - Brown, Mark A1 - Mac Lochlainn, Conchúr A1 - Topali, Paraskevi A1 - Chounta, Irene-Angelica A1 - Ortega-Arranz, Alejandro A1 - Villagrá-Sobrino, Sara L. A1 - Martínez-Monés, Alejandra A1 - Blackwell, Virginia Katherine A1 - Wiltrout, Mary Ellen A1 - Rami Gaddem, Mohamed A1 - Hernández Reyes, César Augusto A1 - Nagahama, Toru A1 - Buchem, Ilona A1 - Okatan, Ebru A1 - Khalil, Mohammad A1 - Casiraghi, Daniela A1 - Sancassani, Susanna A1 - Brambilla, Federica A1 - Mihaescu, Vlad A1 - Andone, Diana A1 - Vasiu, Radu A1 - Şahin, Muhittin A1 - Egloffstein, Marc A1 - Bothe, Max A1 - Rohloff, Tobias A1 - Schenk, Nathanael A1 - Schwerer, Florian A1 - Ifenthaler, Dirk A1 - Hense, Julia A1 - Bernd, Mike ED - Meinel, Christoph ED - Staubitz, Thomas ED - Schweiger, Stefanie ED - Friedl, Christian ED - Kiers, Janine ED - Ebner, Martin ED - Lorenz, Anja ED - Ubachs, George ED - Mongenet, Catherine ED - Ruipérez-Valiente, José A. ED - Cortes Mendez, Manoel T1 - EMOOCs 2021 N2 - From June 22 to June 24, 2021, Hasso Plattner Institute, Potsdam, hosted the seventh European MOOC Stakeholder Summit (EMOOCs 2021) together with the eighth ACM Learning@Scale Conference. Due to the COVID-19 situation, the conference was held fully online. The boost in digital education worldwide as a result of the pandemic was also one of the main topics of this year’s EMOOCs. All institutions of learning have been forced to transform and redesign their educational methods, moving from traditional models to hybrid or completely online models at scale. The learnings, derived from practical experience and research, have been explored in EMOOCs 2021 in six tracks and additional workshops, covering various aspects of this field. In this publication, we present papers from the conference’s Experience Track, the Policy Track, the Business Track, the International Track, and the Workshops. KW - e-learning KW - microcredential KW - MOOC KW - digital education KW - experience KW - online course design KW - online course creation KW - higher education Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-510300 SN - 978-3-86956-512-5 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Fernandez-Fernandez, Jaime A1 - Moya-Ramon, Manuel A1 - Santos-Rosa, Francisco Javier A1 - Gantois, Petrus A1 - Nakamura, Fabio Yuzo A1 - Sanz-Rivas, David A1 - Granacher, Urs T1 - Within-session sequence of the tennis serve training in youth elite players JF - International journal of environmental research and public health N2 - The influence of muscular fatigue on tennis serve performance within regular training sessions is unclear. Therefore, the aim of the present study was to examine the within-session sequence of the tennis serve in youth tennis. Twenty-five young male (14.9 +/- 0.9 years) and female (14.5 +/- 0.9 years) players participated in this within-subject crossover study, and they were randomly but sex-matched assigned to different training sequences (serve exercise before tennis training (BTS) or after tennis training (ATS)). Pre- and post-tests included serve velocity performance and accuracy, shoulder strength, and range-of-motion (ROM) performance (internal/external rotation). Results showed that after one week of serve training conducted following the ATS sequence, significant decreases were found in serve performance (e.g., speed and accuracy), with standardized differences ranging from d = 0.29 to 1.13, as well as the shoulder function (strength [d = 0.20 to 1.0] and ROM [d = 0.17 to 0.31]) in both female and male players, compared to the BTS sequence. Based on the present findings, it appears more effective to implement serve training before the regular tennis training in youth players. If applied after training, excessive levels of fatigue may cause shoulder imbalances that could be related to an increased injury risk. KW - athletes KW - athletic performance KW - fatigue KW - fitness KW - shoulder strength KW - range of motion Y1 - 2020 U6 - https://doi.org/10.3390/ijerph18010244 SN - 1660-4601 VL - 18 IS - 1 PB - MDPI CY - Basel ER -