TY  - JOUR
A1  - Holý, Václav
A1  - Darhuber, A.
A1  - Stangl, Jochen
A1  - Zerlauth, S.
A1  - Schäffler, F.
A1  - Bauer, Günther
A1  - Darowski, Nora
A1  - Lübbert, Daniel
A1  - Pietsch, Ullrich
A1  - Vavra, I.
T1  - HRXRD and GID investigations of a self-organized SiGe quantum dot multilayer
Y1  - 1999
ER  - 
TY  - GEN
A1  - Lang, Judith
A1  - Bohn, Patrick
A1  - Bhat, Hilal
A1  - Jastrow, Holger
A1  - Walkenfort, Bernd
A1  - Cansiz, Feyza
A1  - Fink, Julian
A1  - Bauer, Michael
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Lang, Karl S.
T1  - Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1400 
KW  - immunology
KW  - infection
KW  - membrane fusion
KW  - phagocytosis
KW  - sphingolipids
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-515661
SN  - 1866-8372
IS  - 1
ER  - 
TY  - JOUR
A1  - Lang, Judith
A1  - Bohn, Patrick
A1  - Bhat, Hilal
A1  - Jastrow, Holger
A1  - Walkenfort, Bernd
A1  - Cansiz, Feyza
A1  - Fink, Julian
A1  - Bauer, Michael
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Lang, Karl S.
T1  - Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease
JF  - Nature Communications
N2  - Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol.
KW  - immunology
KW  - infection
KW  - membrane fusion
KW  - phagocytosis
KW  - sphingolipids
Y1  - 2020
U6  - https://doi.org/10.1038/s41467-020-15072-8
SN  - 2041-1723
VL  - 11
IS  - 1
SP  - 1
EP  - 15
PB  - Nature Publishing Group UK
CY  - London
ER  - 
TY  - JOUR
A1  - Zen, Achmad
A1  - Neher, Dieter
A1  - Bauer, C.
A1  - Asawapirom, Udom
A1  - Scherf, Ullrich
A1  - Hagen, R.
A1  - Kostromine, S.
A1  - Mahrt, R. F.
T1  - Polarization-sensitive photoconductivity in aligned polyfluorene layers
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Bauer, C.
A1  - Böhmer, Roland
A1  - Moreno-Flores, S.
A1  - Richert, R.
A1  - Sillescu, H.
A1  - Neher, Dieter
T1  - Capacitive scanning dilatometry and frequency dependent thermal expansion of polymer films
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Ratzloff, Jeffrey K.
A1  - Barlow, Brad N.
A1  - Kupfer, Thomas
A1  - Corcoran, Kyle A.
A1  - Geier, Stephan
A1  - Bauer, Evan
A1  - Corbett, Henry T.
A1  - Howard, Ward S.
A1  - Glazier, Amy
A1  - Law, Nicholas M.
T1  - EVR-CB-001: An Evolving, Progenitor, White Dwarf Compact Binary Discovered with the Evryscope
JF  - The astrophysical journal : an international review of spectroscopy and astronomical physics
N2  - We present EVR-CB-001, the discovery of a compact binary with an extremely low-mass (0.21 +/- 0.05M(circle dot)) helium core white dwarf progenitor (pre-He WD) and an unseen low-mass (0.32 +/- 0.06M(circle dot)) helium white dwarf (He WD) companion. He WDs are thought to evolve from the remnant helium-rich core of a main-sequence star stripped during the giant phase by a close companion. Low-mass He WDs are exotic objects (only about 0.2% of WDs are thought to be less than 0.3 M-circle dot), and are expected to be found in compact binaries. Pre-He WDs are even rarer, and occupy the intermediate phase after the core is stripped, but before the star becomes a fully degenerate WD and with a larger radius (approximate to 0.2R(circle dot)) than a typical WD. The primary component of EVR-CB-001 (the pre-He WD) was originally thought to be a hot subdwarf (sdB) star from its blue color and under-luminous magnitude, characteristic of sdBs. The mass, temperature (T-eff = 18,500 +/- 500 K), and surface gravity (log(g) = 4.96 +/- 0.04) solutions from this work are lower than values for typical hot subdwarfs. The primary is likely to be a post-red-giant branch, pre-He WD contracting into a He WD, and at a stage that places it nearest to sdBs on color-magnitude and T-eff-log(g) diagrams. EVR-CB-001 is expected to evolve into a fully double degenerate, compact system that should spin down and potentially evolve into a single hot subdwarf star. Single hot subdwarfs are observed, but progenitor systems have been elusive.
Y1  - 2019
U6  - https://doi.org/10.3847/1538-4357/ab3727
SN  - 0004-637X
SN  - 1538-4357
VL  - 883
IS  - 1
PB  - IOP Publ. Ltd.
CY  - Bristol
ER  -